yhcV

yhcV
168

forespore-specific sporulation protein

Locus
BSU_09230
Molecular weight
14.77 kDa
Isoelectric point
5.01
Protein length
140 aaSequenceBlast
Gene length
423 bpSequenceBlast
Function
unknown
Product
unknown
Essential
no
Synonyms
yhcV
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG0517 (Galperin et al., 2021)

This gene is a member of the following regulons

SigG regulon

Gene
Coordinates
997,175 → 997,597
The protein
Domains
2 CBS domains (aa 8-64, aa 72-127) (according to UniProt)
Structure
4FRY (PDB) (protein from Burkholderia ambifaria, 30% identity) PubMed
P54606 (AlphaFold)
Paralogous protein(s)
YlbB, (40%)
Additional information
The gene is annotated in KEGG as an ortholog of IMP dehydrogenase EC 1.1.1.205. No EC annotation is available in Swiss-Prot/MetaSwiss-Prot/MetaCyc.literature/experimental evidence supporting the annotation is available. PubMed
Expression and Regulation
Operons
Genes
yhcV
Description
PubMed
Regulation
expressed during sporulation in the forespore (SigG) PubMed
Sigma factors
SigG: sigma factor, PubMed, in sigG regulon
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yhcV

2025-03-25 17:19:02

ghost

94

d05189296f00c818e951c88570579fec963c65f9

75F33A2A8BFFD48DEBCD4E4C948ADB2879084747

Additional information
the mRNA belongs to the 46 most abundant mRNA species in dormant spores PubMed
Biological materials
Mutant
MGNA-B474 (yhcV::erm), available at the NBRP B. subtilis, Japan
BKE09230 (ΔyhcV::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTCAGCACCCCTTTCA,  downstream forward: _UP4_TAATAAGAAAGAGCTTGCAG
BKK09230 (ΔyhcV::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTCAGCACCCCTTTCA,  downstream forward: _UP4_TAATAAGAAAGAGCTTGCAG
Expression vectors
pGP2930 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
References
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Page visits: 5735

Time of last update: 2025-04-03 16:52:50

Author of last update: Jstuelk