pksE

pksE
168

involved in polyketide synthesis

Locus
BSU_17120
Molecular weight
85.61 kDa
Isoelectric point
7.38
Protein length
767 aaSequenceBlast
Gene length
2304 bpSequenceBlast
Function
polyketide synthesis
Product
unknown
Essential
no
Synonyms
pksE
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2070 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,786,104 → 1,788,407
The protein
Catalyzed reaction/ biological activity
holo-[ACP] + malonyl-CoA --> CoA + malonyl-[ACP] (according to UniProt)
Protein family
fabD family (with PksC and FabD, according to UniProt)
Structure
5DZ7 (PDB) (N-terminal domain, aa 1 ... 288)
4CW5 (PDB) (C-terminal domain, from B. amyloliquefaciens, corresponds to aa 315 ... 753, 59% identity) PubMed
O34787 (AlphaFold)
Expression and Regulation
Operons
Genes
pksC-pksD-pksE-acpK-pksF-pksG-pksH-pksI-pksJ-pksL-pksM-pksN-pksR
Description
PubMed
Regulation
expressed during the transition from growth to stationary phase (AbrB, CodY) PubMed
heterogeneous expression, percentage of expressing cells increases during colonization of plant roots PubMed
Regulatory mechanism
AbrB: repression, PubMed, in abrB regulon
CodY: activation, PubMed, in codY regulon
Additional information
this is a very large operon comprising about 75 kb
Open in new tab

pksC→pksR

2025-03-17 18:36:51

Jstuelk

162

74BF817D9000509D225BBBCAC6C8A0A9EABEB843

1475C94AAA2C10C6BE3CA0873EFBD171EC2E2513

Genes
pksB-pksC-pksD-pksE-acpK-pksF-pksG-pksH-pksI-pksJ-pksL-pksM-pksN-pksR
Description
PubMed
Open in new tab

pksB→pksR

2025-03-17 03:33:23

Jstuelk

136

c851e07b7201353cf026d8929ea66cff6bbb92e2

D95CF77A7673AA84CC653095A1FB017D490049D6

Biological materials
Mutant
BKE17120 (ΔpksE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTGCCCTGGAAAGACATATG,  downstream forward: _UP4_TGAATATTATGAAAATATAC
BKK17120 (ΔpksE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTGCCCTGGAAAGACATATG,  downstream forward: _UP4_TGAATATTATGAAAATATAC
References
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CB0B856B22984DD5B10F01DD07C3961FAA742923

Page visits: 4149

Time of last update: 2025-04-01 20:26:39

Author of last update: Melvin.boenninger