rppH
168

decapping of NAD-capped RNAs, part of the error prevention oxidized guanine system (with MutM and MutY), confers protection against oxidative stress to vegetative cells, moreover the protein has RNA pyrophosphohydrolase activity,

Locus

BSU_30630

Molecular weight

18.37 kDa

Isoelectric point

5.95

Protein length

158 aaSequenceBlast

Gene length

477 bpSequenceBlast

Function

DNA repair, RNA degradation

Product

8-oxo-dGTPase (antimutator), RNA pyrophosphohydrolase

Essential

Synonyms

rppH, mutTA, ytkD

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1051 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

3,134,983 3,135,459

The protein

Catalyzed reaction/ biological activity

removal of pyrophosphate from the 5' end of mRNAs to make RNA accessible for degradation by RNases PubMed

RppH requires at least two unpaired nucleotides at the 5' end of its RNA substrates and prefers three or more. The second of these 5'-terminal nucleotides must be G, whereas a less strict preference for a purine is evident at the third position, and A is slightly favored over G at the first position PubMed

decapping of NAD-capped RNAs PubMed

8-oxo-dGTP + H₂O --> 8-oxo-dGMP + diphosphate + H⁺ (according to UniProt)

Protein family

Nudix hydrolase (according to UniProt)

Domains

Nudix hydrolase domain (aa 6-145) (according to UniProt)

Structure

4JZV (PDB) PubMed

O35013 (AlphaFold)

Expression and Regulation

Operons

Genes