miaA

miaA
168

tRNA isopentenylpyrophosphate transferase

Locus
BSU_17330
Molecular weight
35.52 kDa
Isoelectric point
6
Protein length
314 aaSequenceBlast
Gene length
945 bpSequenceBlast
Function
tRNA modification
Product
tRNA isopentenylpyrophosphate transferase
Essential
no
E.C.
2.5.1.8
Synonyms
miaA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0324 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,866,389  1,867,333
Phenotypes of a mutant
inactivation of ''miaA'' reduces sporulation efficiency to 16% that of wild type cells PubMed
The protein
Catalyzed reaction/ biological activity
adenosine37 in tRNA + dimethylallyl diphosphate --> diphosphate + N6-dimethylallyladenosine37 in tRNA (according to UniProt)
Protein family
IPP transferase family (single member, according to UniProt)
Structure
2QGN (PDB) (from Bacillus halodurans, 57% identity)
O31795 (AlphaFold)
Expression and Regulation
Operons
Genes
miaA-hfq
Description
PubMed
Open in new tab

miaA→hfq

2025-03-16 08:15:34

Jstuelk

113

618ff8dc028666b7102a8ac70fd2e0e7e7cee80a

30921149A989DB9C49A175051521F780FA3F893A

Genes
gerY-miaA-hfq
Description
PubMed
Regulation
expressed during sporulation in the mother cell (SigE) PubMed
Sigma factors
SigE: sigma factor, PubMed, in sigE regulon
Open in new tab

gerY→hfq

2025-03-16 01:24:45

Jstuelk

122

4C22FBFE04A5390FCBAE1A84C6631B5B7D9CC3D4

414C28CC98280BCBBDFD5DED7555018FF98C974C

Biological materials
Mutant
BKE17330 (miaA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATATGTATCACTCTCTTT,  downstream forward: _UP4_TAATCGAAACTGTATGATAT
BKK17330 (miaA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATATGTATCACTCTCTTT,  downstream forward: _UP4_TAATCGAAACTGTATGATAT
References
The corresponding gene/ protein in other bacteria
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09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D

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Time of last update: 2025-04-03 08:13:23

Author of last update: Jstuelk