ktrD

ktrD
168

glutamate-controlled potassium channel KtrC-KtrD, integral membrane subunit

Locus
BSU_13500
Molecular weight
49.26 kDa
Isoelectric point
9.22
Protein length
449 aaSequenceBlast
Gene length
1350 bpSequenceBlast
Function
potassium uptake
Product
glutamate-controlled potassium channel KtrC-KtrD, integral membrane subunit
Essential
no
Synonyms
ktrD, ykrM
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0168 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,416,067  1,417,416
The protein
Catalyzed reaction/ biological activity
uptake of potassium
Protein family
TrkH potassium transport family (with KtrB, according to UniProt)
Structure
4J7C (PDB) (the KtrA-KtrB complex, 36% identity) PubMed
O31658 (AlphaFold)
Effectors of protein activity
the affinity of KtrC-KtrD for potassium is strongly increased in the presence of glutamate PubMed
Kinetic information
the KtrC-KtrD channel has a low affinity for potassium,this is determined by KtrD PubMed
the affinity of KtrC-KtrD for potassium is strongly increased in the presence of glutamate (the KD for potassium is decreased from 0.278 mM to 0.017 mM) PubMed
Paralogous protein(s)
KtrB
Localization
integral membrane protein PubMed
Expression and Regulation
Operons
Genes
ktrD
Description
PubMed
Regulation
constitutively expressed
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ktrD

2025-03-29 10:52:09

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Biological materials
Mutant
MGNA-B317 (ykrM::erm), available at the NBRP B. subtilis, Japan
GHB12 (ktrD::tet), PubMed, available in Erhard Bremer's and Jörg Stülke's labs
GP2030 (ktrD::tet), available in  Jörg Stülke's lab PubMed
GP3646 (ktrD::kan), available in  Jörg Stülke's lab
GP4184 (ktrD::spec), available in  Jörg Stülke's lab
1A956 (ktrD::tet), PubMed, available at BGSC
BKE13500 (ktrD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTAAAATCCTTCTGCCT,  downstream forward: _UP4_TAGGCAAAACACCGCATATT
BKK13500 (ktrD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTAAAATCCTTCTGCCT,  downstream forward: _UP4_TAGGCAAAACACCGCATATT
Expression vectors
pGP2996 (N-terminal 6xHis-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
FLAG-tag construct
GP3465 ktrD-3xFLAG spc (based on pGP1331), available in Jörg Stülke's lab
Labs working on this gene/protein
Erhard Bremer, University of Marburg, Germany Homepage
Inga Hänelt, Frankfurt, Germany Homepage
João H Morais-Cabral, University of Porto, Portugal Homepage
Jörg Stülke, University of Göttingen, Germany Homepage
References
Reviews
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Original Publications
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Page visits: 4757

Time of last update: 2025-04-02 00:33:07

Author of last update: Jstuelk