htrB

htrB
168

membrane-anchored protein quality control protease, serine protease, response to secretion and heat stresses

Locus
BSU_33000
Molecular weight
48.55 kDa
Isoelectric point
4.63
Protein length
458 aaSequenceBlast
Gene length
1377 bpSequenceBlast
Function
protein quality control
Product
serine protease
Essential
no
E.C.
3.4.21.107
Synonyms
htrB, yvtA, yvtB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0265 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,384,070  3,385,446
The protein
Catalyzed reaction/ biological activity
Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val (according to UniProt)
Protein family
Peptidase S1C family (with HtrA and HtrC, according to UniProt)
Domains
cytoplasmic domain (aa 1 - 71) (according to UniProt)
transmembrane helix (aa 72 - 92) (according to UniProt)
extracellular domain (aa 93 - 458) (according to UniProt)
PDZ domain (aa 350- 449) (according to UniProt)
Structure
3QO6 (PDB) (from Arabidopsis thaliana, 38% identity) PubMed
Q9R9I1 (AlphaFold)
Localization
cell membrane (according to UniProt)
randomly distributed in foci throughout the cell surface PubMed
Expression and Regulation
Operons
Genes
htrB
Description
PubMed
Regulation
expressed under conditions of secretion stress (CssR) PubMed
Regulatory mechanism
CssR: activation, PubMed, in cssR regulon
Additional information
HtrB is subject to degradation by WprA and other extracellular proteases PubMed
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htrB

2025-03-14 23:39:21

Jstuelk

88

1bdf6477a0252008ca17ec508e821d917ca520de

A76E2442C553874A294760AC665F8984888782DE

Biological materials
Mutant
MGNA-B207 (yvtA::erm), available at the NBRP B. subtilis, Japan
BKE33000 (htrB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTCTTACACTCCTTTA,  downstream forward: _UP4_TAAGAAAAAACAAAAAGCTG
BKK33000 (htrB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTCTTACACTCCTTTA,  downstream forward: _UP4_TAAGAAAAAACAAAAAGCTG
References
Reviews
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Original Publications
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796216BE9CD6DADFEADC29497253C81BF496A2ED

Page visits: 4535

Time of last update: 2025-04-02 08:29:25

Author of last update: Jstuelk