hisA

hisA
168

phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase, homologue to HisF

Locus
BSU_34880
Molecular weight
26.38 kDa
Isoelectric point
4.84
Protein length
245 aaSequenceBlast
Gene length
738 bpSequenceBlast
Function
biosynthesis of histidine
Product
phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase, homologue to HisF
Essential
no
E.C.
5.3.1.16
Synonyms
hisA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG0106 (Galperin et al., 2021)

This gene is a member of the following regulons

RnpM regulon

Gene
Coordinates
3,584,317  3,585,054
The protein
Catalyzed reaction/ biological activity
1-(5-phospho-β-D-ribosyl)-5-[(5-phospho-β-D-ribosylamino)methylideneamino]imidazole-4-carboxamide --> 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamide (according to UniProt)
Protein family
HisA/HisF family (with HisF, according to UniProt)
Structure
2VEP (PDB) (from Streptomyces coelicolor, 41% identity, 58% similarity) PubMed
O35006 (AlphaFold)
Paralogous protein(s)
HisF
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
hisZ-hisG-hisD-hisB-hisH-hisA-hisF-hisI
Description
PubMed
Regulation
expression during spore germination is strongly reduced under conditions of osmotic stress PubMed
expression depends on functional RnpM PubMed
Regulatory mechanism
RnpM: unknown, PubMed, in rnpM regulon
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hisZ→hisI

2025-04-03 14:03:13

Jstuelk

133

896e17f5309df81d9427f38fec175515ad428723

B6AAF7601668444EDDC2FE084A87885740B98ACD

Biological materials
Mutant
1A626 ( hisA::erm), PubMed, available at BGSC
BKE34880 (hisA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGCCGGATATAATGTAAAAG,  downstream forward: _UP4_GCGCTTGAAAGGGTGAAGCG
BKK34880 (hisA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGCCGGATATAATGTAAAAG,  downstream forward: _UP4_GCGCTTGAAAGGGTGAAGCG
References
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Page visits: 2854

Time of last update: 2025-04-03 16:18:02

Author of last update: Melvin.boenninger