cwlQ

cwlQ
168

putative cell wall hydrolase (N-acetylglucosamidase), required for swarming motility

Locus
BSU_11570
Molecular weight
18.85 kDa
Isoelectric point
7.01
Protein length
181 aaSequenceBlast
Gene length
546 bpSequenceBlast
Function
cell wall turnover
Product
lytic transglycosylase
Essential
no
Synonyms
cwlQ, yjbJ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0741 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,235,163 → 1,235,708
Phenotypes of a mutant
severe defect in flagellar-dependent Swarming motility PubMed
The protein
Catalyzed reaction/ biological activity
bifunctional cell wall hydrolase with muramidase and soluble-lytic transglycosylase activities PubMed
Protein family
transglycosylase Slt family (together with CwlP) (according to UniProt)
Domains
unkown N-terminal domain PubMed
C-terminal lytic transglycosidase domain (aa 63 - 244) PubMed
Structure
5OHU (PDB) (from Pseudomonas aeruginosa, corresponds to aa 62 ... 177, 37% identity) PubMed
O31608 (AlphaFold)
Localization
secreted by the flagellar type III secretion system involving FliF PubMed
Expression and Regulation
Operons
Genes
cwlQ
Description
PubMed
Sigma factors
SigD: sigma factor, PubMed, in sigD regulon
Open in new tab

cwlQ

2025-02-27 19:37:23

ghost

74

060c03d2b9927ca3ba189d1bdd9200c81d731ae1

B18DD478E6CE4A342E98F86FF593B00945146C25

Genes
yjbK-cwlQ
Description
PubMed
Open in new tab

yjbK→cwlQ

2025-03-14 19:19:25

Jstuelk

75

fc6bbba29265345014e99da944ae0e1800e79f98

F686CB4F40746272B66DB966F83FD64C002C491E

Biological materials
Mutant
MGNA-B156 (yjbJ::erm), available at the NBRP B. subtilis, Japan
BKE11570 (ΔcwlQ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTGAGCGGGCTTGGCG,  downstream forward: _UP4_TAACCAGAGGTGCTCTTTCC
BKK11570 (ΔcwlQ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTGAGCGGGCTTGGCG,  downstream forward: _UP4_TAACCAGAGGTGCTCTTTCC
References
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BD3C36B63C5D88FDBACFB18849F3DA863F38383F

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Time of last update: 2025-04-02 13:36:13

Author of last update: Jstuelk