cspR

cspR
168

tRNA (Um34/Cm34) methyltransferase

Locus
BSU_08930
Molecular weight
18.44 kDa
Isoelectric point
6.05
Protein length
160 aaSequenceBlast
Gene length
483 bpSequenceBlast
Function
translation
Product
tRNA (Um34/Cm34) methyltransferase
Essential
no
E.C.
2.1.1.-
Synonyms
cspR, ygaR, trmL
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0219 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
970,135  970,617
Phenotypes of a mutant
essential PubMed, non-essential according to PubMed
The protein
Catalyzed reaction/ biological activity
catalyzes the methyl transfer from S-adenosyl-L-methionine to tRNA(Leu)(CAA) and tRNA(Leu)(UAA) isoacceptors PubMed
cytidine34 in tRNA + S-adenosyl-L-methionine --> 2'-O-methylcytidine34 in tRNA + H+ + S-adenosyl-L-homocysteine (according to UniProt)
5-carboxymethylaminomethyluridine34 in tRNALeu + S-adenosyl-L-methionine --> 5-carboxymethylaminomethyl-2'-O-methyluridine34 in tRNALeu + H+ + S-adenosyl-L-homocysteine (according to UniProt)
Protein family
Methyltransferase superfamily (according to UniProt)
class IV-like SAM-binding methyltransferase superfamily (with YacO and RlmP, according to UniProt)
Structure
4JAK (PDB) (from E. coli, 45% identity, 72% similarity) PubMed
O31590 (AlphaFold)
Expression and Regulation
Operons
Genes
yhbB-cspR
Description
PubMed
Regulation
''yhbB-cspR'': expressed in the mother cell early during sporulation (SigE) PubMed
Sigma factors
SigE: sigma factor, PubMed, in sigE regulon
Open in new tab

yhbB→cspR

2025-03-25 17:58:59

ghost

99

29b96ca9161089a03a179025bb3ceee0647ec726

5B631CC6ACD9F6DCA4410FD54B73BD6CAF63882A

Genes
cspR
Description
PubMed
Regulation
''yhbB-cspR'': expressed in the mother cell early during sporulation (SigE) PubMed
Open in new tab

cspR

2025-03-10 02:53:00

ghost

109

0d8447cdc61b9de5b89e45a9fd00a5d7ee8731ec

9FF31973E2083617EC131EB97918554671B0A792

Biological materials
Mutant
BKE08930 (cspR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACCTCTGTTTTTCTTATCC,  downstream forward: _UP4_TAGTGAAAAACCCGCTCATC
BKK08930 (cspR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACCTCTGTTTTTCTTATCC,  downstream forward: _UP4_TAGTGAAAAACCCGCTCATC
References
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03BA7AEE17739689D632A35D9C3329EA0982C98A

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Time of last update: 2025-04-02 06:13:42

Author of last update: Jstuelk