bkdB
168

2-oxoisovalerate dehydrogenase (E2 subunit, lipoamide acyltransferase)

Locus

BSU_24030

Molecular weight

45.67 kDa

Isoelectric point

5.3

Protein length

424 aaSequenceBlast

Gene length

1275 bpSequenceBlast

Function

utilization of branched-chain keto acids

Product

2-oxoisovalerate dehydrogenase (E2 subunit, lipoamide acyltransferase)

Essential

E.C.

2.3.1.168

Synonyms

bkdB, bfmBB, bfmB2, bkd

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0508 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

2,496,796 2,498,070

Phenotypes of a mutant

a strain lacking the ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB operon and the des gene exhibits strongly reduced membrane fluidity and eventual growth arrest PubMed

The protein

Catalyzed reaction/ biological activity

(R)-N⁶-dihydrolipoyl-L-lysyl-[protein] + 2-methylpropanoyl-CoA --> (R)-N⁶-(S⁸-2-methylpropanoyldihydrolipoyl)-L-lysyl-[protein] + CoA (according to UniProt)

Protein family

2-oxoacid dehydrogenase family (according to UniProt)

Domains

Lipoyl-binding domain (aa 3-78) (according to UniProt)

Peripheral subunit-binding domain (PSBD) (aa 116-153) (according to UniProt)

Cofactors

lipoic acid (on Lys-44), can probably be removed by SrtN PubMed

Structure

3DUF (PDB) (Geobacillus stearothermophilus PdhC, 36% identity) PubMed

P37942 (AlphaFold)

Paralogous protein(s)

PdhC, AcoC, OdhB