FapR

2009-07-02T20:51:41Z

Schujman: /* Expression and regulation */

* '''Description:''' repressor of fatty acid synthetic genes <br/><br/>

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''fapR ''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylpC''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional repressor
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of fatty acid biosynthesis
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 21 kDa, 5.393
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 564 bp, 188 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[recG]]'', ''[[plsX]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13461]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:ylpC_context.gif]]
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
|-
|}

__TOC__

<br/><br/>

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU15880

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fapR-plsX-fabDG.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13400]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' fapR family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:''' malonyl-CoA, acts as the molecular inducer of the FapR regulon

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2F3X 2F3X] (complex with effector), [http://www.rcsb.org/pdb/explore.do?structureId=2F41 2F41]

* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O34835 O34835]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15880]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=
FapR regulates the expression of at least 10 genes (the ''fap'' regulon). It autoregulates its own expression. Malonyl-CoA, a precursor of fatty acid biosynthesis, binds to FapR changing its conformation to a non-DNA binding state. Hence, conditions that cause malonyl-CoA accumulation, like fatty acid biosynthesis inhibition, derepress the ''fap'' regulon.
* '''Operon:'''

* '''[[Sigma factor]]:'''
fapR operon is under the control of SigA and FapR
* '''Regulation:'''
** repressed unless the cells experience an accumulation of malonyl-CoA ([[FapR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12737802 PubMed]

* '''Regulatory mechanism:'''
** [[FapR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12737802 PubMed]

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>12737802, 18941141, 18820022, 18384517, 17919287, 16932747,12737802 </pubmed>

SubtiWiki - User contributions [en]

FapR