CggR

2010-05-06T08:42:20Z

Paola mybhaby:

* '''Description:''' repressor of the glycolytic ''[[gapA]]'' operon, [[DeoR family]] 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''cggR''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yvbQ ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || central glycolytic genes regulator
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || transcriptional regulator
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37,2 kDa,5.68
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1020 bp, 340 amino acids
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gapA]]'', ''[[araE]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15400]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:cggR_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU33950

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cggR-gapA-pgk-tpiA-pgm-eno.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG14085]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' transcription repression of the glycolytic ''[[gapA]]'' operon

* '''Protein family:''' sorC transcriptional regulatory family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''
** DNA binding domain (H-T-H motif) (37–56)

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:''' fructose 1.6-bisphosphate [http://www.ncbi.nlm.nih.gov/sites/entrez/12622823 PubMed] and dihydroxyacetone phosphate, glucose-6-phosphate and fructose-6-phosphate [http://www.ncbi.nlm.nih.gov/sites/entrez/18554327 PubMed] act as inducer and result in release of CggR from the DNA

* '''Interactions:'''
** active as dimer (according to {{PubMed|20408793}})

* '''Localization:'''

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2OKG 2OKG] ( effector binding domain), [http://www.rcsb.org/pdb/explore.do?structureId=3BXH 3BXH] (in complex with fructose-6-phosphate), complex with Fructose-6-Phosphate [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=65242 NCBI], effector binding domain [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=44226 NCBI]

* '''UniProt:''' [http://www.uniprot.org/uniprot/O32253 O32253]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU33950]

=== Additional information===

=Expression and regulation=

* '''Operon:'''
** ''[[cggR]]-[[gapA]]-[[pgk]]-[[tpi]]-[[pgm]]-[[eno]]'' {{PubMed|11489127}}
** ''[[cggR]]-[[gapA]]'' {{PubMed|11489127}}

* '''Sigma factor:''' [[SigA]] {{PubMed|11489127}}

* '''Regulation:'''
** expression activated by glucose (77-fold) ([[CggR]]) {{PubMed|12850135,12622823}}

* '''Regulatory mechanism:'''
** ''[[cggR]]'': transcription repression {{PubMed|11489127}}

* '''Database entries:''' [http://dbtbs.hgc.jp/COG/prom/cggR-gapA-pgk-tpiA-pgm-eno.html DBTBS]

* '''Additional information:'''
** The primary mRNAs of the operon are highly unstable. The primary mRNA is subject to processing at the very end of the ''[[cggR]]'' open reading frame. This results in stable mature ''[[gapA]]'' and ''[[gapA]]-[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]'' mRNAs. {{PubMed|11489127}} The processing event requires the [[Rny|RNase Y]] {{PubMed|19193632}}.
** The intracellular concentration of CggR is about 230 nM (according to {{PubMed|20408793}}).

=Biological materials =

* '''Mutant:'''
** GP311 (in frame deletion), available in [[Stülke]] lab
** SM-NB7 (cggR-spc), available in [[Anne Galinier]]'s and [[Boris Görke]]'s labs

* '''Expression vector:''' pGP705 (N-terminal His-tag, in [[pWH844]]), available in [[Stülke]] lab

* '''lacZ fusion:''' pGP504 (in [[pAC7]]), pGP509 (in [[pAC6]]), available in [[Stülke]] lab

* '''GFP fusion:'''

* '''Antibody:''' available in [[Stülke]] lab

=Labs working on this gene/protein=

[[Stephane Aymerich |Stephane Aymerich]], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

=Your additional remarks=

==Related Links:==

[http://www.bodybuildingrevealed.com/'''body building''']

=References=
==Reviews==
<pubmed> 20408793 </pubmed>

==Original Publications==
<pubmed>19193632,12850135,12622823,18186488,10799476,11489127,12123463,12634343, 18554327, 18052209 , 17293407 20361740 </pubmed>

[[Category:Protein-coding genes]]

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CggR