SubtiWiki - User contributions [en]

PtkA

2009-03-15T11:44:08Z

Ivanmijakovic: /* The protein */

* '''Description:''' write here 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ptkA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywqD ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' ||
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 25 kDa, 9.628
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 711 bp, 237 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ptpZ]]'', ''[[tkmA]]''
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ywqD_nucleotide.txt Gene sequence (+200bp) ]'''
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ywqD_protein.txt Protein sequence]'''
|-
|colspan="2" | '''Genetic context''' [[Image:ywqD_context.gif]]
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:'''

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ywqCD.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12508]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' autophosphorylation, phosphorylation of Ugd, TuaD, SsbA, SsbB

* '''Protein family:''' BY-kinase

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:''' single BY-kinase domain

* '''Modification:''' autophosphorylation at residues Y225, Y227 and Y228 (primary site)

* '''Cofactor(s):''' ATP

* '''Effectors of protein activity:''' TkmA - transmembrane modulator, activates PtkA autophosphorylation and substrate phosphorylation

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:'''

* '''KEGG entry:'''

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[tkmA]]-[[ptkA]]-[[ptpZ]]-[[ywqF]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/12970183 PubMed]

* '''Sigma factor:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' KO strain created with pMUTIN-2, available from [[Ivan Mijakovic]]

* '''Expression vector:''' pQE-30, N-terminally 6xHis-tagged, available from [[Ivan Mijakovic]]

* '''lacZ fusion:''' in a KO strain created with pMUTIN-2, available from [[Ivan Mijakovic]]

* '''GFP fusion:''' CFP-fusion, available from [[Ivan Mijakovic]]

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=
[[Ivan Mijakovic]], Thiverval-Grignon, France

=Your additional remarks=

=References=

# Mijakovic I, Poncet S, Boël G, Mazé A, Gillet S, Decottignies P, Grangeasse C, Jamet E, Doublet P, Le Maréchal P, Deutscher J (2003) Transmembrane modulator-dependent bacterial tyrosine kinase activates UDP-glucose dehydrogenases. EMBO J 22:4709-4718. [http://www.ncbi.nlm.nih.gov/sites/entrez/12970183 PubMed]
# Mijakovic I, Petranovic D, Deutscher J (2004) How tyrosine phosphorylation affects the UDP-glucose dehydrogenase activity of Bacillus subtilis YwqF. J Mol Microbiol Biotechnol 8:19-25. [http://www.ncbi.nlm.nih.gov/sites/entrez/15741737 PubMed]
# Mijakovic I, Musumeci L, Tautz L, Petranovic D, Edwards RA, Jensen PR, Mustelin T, Deutscher J, Bottini N (2005) In vitro characterization of the Bacillus subtilis protein tyrosine phosphatase YwqE. J Bacteriol 187(10):3384-3390 [http://www.ncbi.nlm.nih.gov/sites/entrez/15866923 PubMed]
# Mijakovic I, Petranovic D, Macek B, Cepo T, Mann M, Davies J, Jensen PR, Vujaklija D (2006) Bacterial single-stranded DNA-binding proteins are phosphorylated on tyrosine. Nucl Acids Res 34:1588-1596. [http://www.ncbi.nlm.nih.gov/sites/entrez/16549871 PubMed]
# Petranovic D, Michelsen O, Zahradka K, Silva C, Petranovic M, Jensen PR, Mijakovic I (2007) Bacillus subtilis strain deficient for the protein-tyrosine kinase PtkA exhibits impaired DNA replication. Mol Microbiol 63:1797-1805. [http://www.ncbi.nlm.nih.gov/sites/entrez/17367396 PubMed]

PtkA

2009-03-15T11:43:21Z

Ivanmijakovic: /* Extended information on the protein */

* '''Description:''' write here 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ptkA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywqD ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' ||
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 25 kDa, 9.628
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 711 bp, 237 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ptpZ]]'', ''[[tkmA]]''
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ywqD_nucleotide.txt Gene sequence (+200bp) ]'''
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ywqD_protein.txt Protein sequence]'''
|-
|colspan="2" | '''Genetic context''' [[Image:ywqD_context.gif]]
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:'''

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ywqCD.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12508]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' BY-kinase

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:''' single BY-kinase domain

* '''Modification:''' autophosphorylation at residues Y225, Y227 and Y228 (primary site)

* '''Cofactor(s):''' ATP

* '''Effectors of protein activity:''' TkmA - transmembrane modulator, activates PtkA autophosphorylation and substrate phosphorylation

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:'''

* '''KEGG entry:'''

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[tkmA]]-[[ptkA]]-[[ptpZ]]-[[ywqF]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/12970183 PubMed]

* '''Sigma factor:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' KO strain created with pMUTIN-2, available from [[Ivan Mijakovic]]

* '''Expression vector:''' pQE-30, N-terminally 6xHis-tagged, available from [[Ivan Mijakovic]]

* '''lacZ fusion:''' in a KO strain created with pMUTIN-2, available from [[Ivan Mijakovic]]

* '''GFP fusion:''' CFP-fusion, available from [[Ivan Mijakovic]]

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=
[[Ivan Mijakovic]], Thiverval-Grignon, France

=Your additional remarks=

=References=

# Mijakovic I, Poncet S, Boël G, Mazé A, Gillet S, Decottignies P, Grangeasse C, Jamet E, Doublet P, Le Maréchal P, Deutscher J (2003) Transmembrane modulator-dependent bacterial tyrosine kinase activates UDP-glucose dehydrogenases. EMBO J 22:4709-4718. [http://www.ncbi.nlm.nih.gov/sites/entrez/12970183 PubMed]
# Mijakovic I, Petranovic D, Deutscher J (2004) How tyrosine phosphorylation affects the UDP-glucose dehydrogenase activity of Bacillus subtilis YwqF. J Mol Microbiol Biotechnol 8:19-25. [http://www.ncbi.nlm.nih.gov/sites/entrez/15741737 PubMed]
# Mijakovic I, Musumeci L, Tautz L, Petranovic D, Edwards RA, Jensen PR, Mustelin T, Deutscher J, Bottini N (2005) In vitro characterization of the Bacillus subtilis protein tyrosine phosphatase YwqE. J Bacteriol 187(10):3384-3390 [http://www.ncbi.nlm.nih.gov/sites/entrez/15866923 PubMed]
# Mijakovic I, Petranovic D, Macek B, Cepo T, Mann M, Davies J, Jensen PR, Vujaklija D (2006) Bacterial single-stranded DNA-binding proteins are phosphorylated on tyrosine. Nucl Acids Res 34:1588-1596. [http://www.ncbi.nlm.nih.gov/sites/entrez/16549871 PubMed]
# Petranovic D, Michelsen O, Zahradka K, Silva C, Petranovic M, Jensen PR, Mijakovic I (2007) Bacillus subtilis strain deficient for the protein-tyrosine kinase PtkA exhibits impaired DNA replication. Mol Microbiol 63:1797-1805. [http://www.ncbi.nlm.nih.gov/sites/entrez/17367396 PubMed]

PtkA

2009-03-15T11:42:09Z

Ivanmijakovic: /* Biological materials */

* '''Description:''' write here 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ptkA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywqD ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' ||
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 25 kDa, 9.628
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 711 bp, 237 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ptpZ]]'', ''[[tkmA]]''
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ywqD_nucleotide.txt Gene sequence (+200bp) ]'''
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ywqD_protein.txt Protein sequence]'''
|-
|colspan="2" | '''Genetic context''' [[Image:ywqD_context.gif]]
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:'''

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ywqCD.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12508]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' BY-kinase

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:''' autophosphorylation at residues Y225, Y227 and Y228 (primary site)

* '''Cofactor(s):''' ATP

* '''Effectors of protein activity:''' TkmA - transmembrane modulator, activates PtkA autophosphorylation and substrate phosphorylation

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:'''

* '''KEGG entry:'''

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[tkmA]]-[[ptkA]]-[[ptpZ]]-[[ywqF]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/12970183 PubMed]

* '''Sigma factor:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' KO strain created with pMUTIN-2, available from [[Ivan Mijakovic]]

* '''Expression vector:''' pQE-30, N-terminally 6xHis-tagged, available from [[Ivan Mijakovic]]

* '''lacZ fusion:''' in a KO strain created with pMUTIN-2, available from [[Ivan Mijakovic]]

* '''GFP fusion:''' CFP-fusion, available from [[Ivan Mijakovic]]

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=
[[Ivan Mijakovic]], Thiverval-Grignon, France

=Your additional remarks=

=References=

# Mijakovic I, Poncet S, Boël G, Mazé A, Gillet S, Decottignies P, Grangeasse C, Jamet E, Doublet P, Le Maréchal P, Deutscher J (2003) Transmembrane modulator-dependent bacterial tyrosine kinase activates UDP-glucose dehydrogenases. EMBO J 22:4709-4718. [http://www.ncbi.nlm.nih.gov/sites/entrez/12970183 PubMed]
# Mijakovic I, Petranovic D, Deutscher J (2004) How tyrosine phosphorylation affects the UDP-glucose dehydrogenase activity of Bacillus subtilis YwqF. J Mol Microbiol Biotechnol 8:19-25. [http://www.ncbi.nlm.nih.gov/sites/entrez/15741737 PubMed]
# Mijakovic I, Musumeci L, Tautz L, Petranovic D, Edwards RA, Jensen PR, Mustelin T, Deutscher J, Bottini N (2005) In vitro characterization of the Bacillus subtilis protein tyrosine phosphatase YwqE. J Bacteriol 187(10):3384-3390 [http://www.ncbi.nlm.nih.gov/sites/entrez/15866923 PubMed]
# Mijakovic I, Petranovic D, Macek B, Cepo T, Mann M, Davies J, Jensen PR, Vujaklija D (2006) Bacterial single-stranded DNA-binding proteins are phosphorylated on tyrosine. Nucl Acids Res 34:1588-1596. [http://www.ncbi.nlm.nih.gov/sites/entrez/16549871 PubMed]
# Petranovic D, Michelsen O, Zahradka K, Silva C, Petranovic M, Jensen PR, Mijakovic I (2007) Bacillus subtilis strain deficient for the protein-tyrosine kinase PtkA exhibits impaired DNA replication. Mol Microbiol 63:1797-1805. [http://www.ncbi.nlm.nih.gov/sites/entrez/17367396 PubMed]

PtkA

2009-03-15T11:40:43Z

Ivanmijakovic: /* References */

* '''Description:''' write here 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ptkA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywqD ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' ||
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 25 kDa, 9.628
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 711 bp, 237 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ptpZ]]'', ''[[tkmA]]''
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ywqD_nucleotide.txt Gene sequence (+200bp) ]'''
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ywqD_protein.txt Protein sequence]'''
|-
|colspan="2" | '''Genetic context''' [[Image:ywqD_context.gif]]
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:'''

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ywqCD.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12508]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' BY-kinase

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:''' autophosphorylation at residues Y225, Y227 and Y228 (primary site)

* '''Cofactor(s):''' ATP

* '''Effectors of protein activity:''' TkmA - transmembrane modulator, activates PtkA autophosphorylation and substrate phosphorylation

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:'''

* '''KEGG entry:'''

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[tkmA]]-[[ptkA]]-[[ptpZ]]-[[ywqF]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/12970183 PubMed]

* '''Sigma factor:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' KO strain created with pMUTIN-2, available from [[Ivan Mijakovic]]

* '''Expression vector:''' pQE-30, N-terminally 6xHis-tagged, available from [[Ivan Mijakovic]]

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=
[[Ivan Mijakovic]], Thiverval-Grignon, France

=Your additional remarks=

=References=

# Mijakovic I, Poncet S, Boël G, Mazé A, Gillet S, Decottignies P, Grangeasse C, Jamet E, Doublet P, Le Maréchal P, Deutscher J (2003) Transmembrane modulator-dependent bacterial tyrosine kinase activates UDP-glucose dehydrogenases. EMBO J 22:4709-4718. [http://www.ncbi.nlm.nih.gov/sites/entrez/12970183 PubMed]
# Mijakovic I, Petranovic D, Deutscher J (2004) How tyrosine phosphorylation affects the UDP-glucose dehydrogenase activity of Bacillus subtilis YwqF. J Mol Microbiol Biotechnol 8:19-25. [http://www.ncbi.nlm.nih.gov/sites/entrez/15741737 PubMed]
# Mijakovic I, Musumeci L, Tautz L, Petranovic D, Edwards RA, Jensen PR, Mustelin T, Deutscher J, Bottini N (2005) In vitro characterization of the Bacillus subtilis protein tyrosine phosphatase YwqE. J Bacteriol 187(10):3384-3390 [http://www.ncbi.nlm.nih.gov/sites/entrez/15866923 PubMed]
# Mijakovic I, Petranovic D, Macek B, Cepo T, Mann M, Davies J, Jensen PR, Vujaklija D (2006) Bacterial single-stranded DNA-binding proteins are phosphorylated on tyrosine. Nucl Acids Res 34:1588-1596. [http://www.ncbi.nlm.nih.gov/sites/entrez/16549871 PubMed]
# Petranovic D, Michelsen O, Zahradka K, Silva C, Petranovic M, Jensen PR, Mijakovic I (2007) Bacillus subtilis strain deficient for the protein-tyrosine kinase PtkA exhibits impaired DNA replication. Mol Microbiol 63:1797-1805. [http://www.ncbi.nlm.nih.gov/sites/entrez/17367396 PubMed]

PtkA

2009-03-15T11:26:29Z

Ivanmijakovic: /* Biological materials */

* '''Description:''' write here 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ptkA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywqD ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' ||
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 25 kDa, 9.628
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 711 bp, 237 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ptpZ]]'', ''[[tkmA]]''
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ywqD_nucleotide.txt Gene sequence (+200bp) ]'''
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ywqD_protein.txt Protein sequence]'''
|-
|colspan="2" | '''Genetic context''' [[Image:ywqD_context.gif]]
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:'''

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ywqCD.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12508]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' BY-kinase

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:''' autophosphorylation at residues Y225, Y227 and Y228 (primary site)

* '''Cofactor(s):''' ATP

* '''Effectors of protein activity:''' TkmA - transmembrane modulator, activates PtkA autophosphorylation and substrate phosphorylation

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:'''

* '''KEGG entry:'''

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[tkmA]]-[[ptkA]]-[[ptpZ]]-[[ywqF]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/12970183 PubMed]

* '''Sigma factor:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' KO strain created with pMUTIN-2, available from [[Ivan Mijakovic]]

* '''Expression vector:''' pQE-30, N-terminally 6xHis-tagged, available from [[Ivan Mijakovic]]

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=
[[Ivan Mijakovic]], Thiverval-Grignon, France

=Your additional remarks=

=References=

# Mijakovic I, Poncet S, Boël G, Mazé A, Gillet S, Decottignies P, Grangeasse C, Jamet E, Doublet P, Le Maréchal P, Deutscher J (2003) Transmembrane modulator-dependent bacterial tyrosine kinase activates UDP-glucose dehydrogenases. EMBO J 22:4709-4718. [http://www.ncbi.nlm.nih.gov/sites/entrez/12970183 PubMed]
# Mijakovic I, Petranovic D, Deutscher J (2004) How tyrosine phosphorylation affects the UDP-glucose dehydrogenase activity of Bacillus subtilis YwqF. J Mol Microbiol Biotechnol 8:19-25. [http://www.ncbi.nlm.nih.gov/sites/entrez/15741737 PubMed]
# Mijakovic I, Petranovic D, Macek B, Cepo T, Mann M, Davies J, Jensen PR, Vujaklija D (2006) Bacterial single-stranded DNA-binding proteins are phosphorylated on tyrosine. Nucl Acids Res 34:1588-1596. [http://www.ncbi.nlm.nih.gov/sites/entrez/16549871 PubMed]
# Petranovic D, Michelsen O, Zahradka K, Silva C, Petranovic M, Jensen PR, Mijakovic I (2007) Bacillus subtilis strain deficient for the protein-tyrosine kinase PtkA exhibits impaired DNA replication. Mol Microbiol 63:1797-1805. [http://www.ncbi.nlm.nih.gov/sites/entrez/17367396 PubMed]

Ivan Mijakovic

2009-03-15T11:25:10Z

Ivanmijakovic:

= Lab head: Ivan Mijakovic=
'''Additional information'''
* [mailto:Ivan.Mijakovic@grignon.inra.fr send mail]
* location: Thiverval-Grignon, France
* Homepage: not available

= Publications =
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed&term=%22Mijakovic%2BI%22%5Bau%5D&dispmax=50 Pubmed]

= Main research interests =

protein phosphorylation, phosphoproteomics, tyrosine kinases

= Open positions =

PtkA

2009-03-15T11:24:11Z

Ivanmijakovic: /* Labs working on this gene/protein */

* '''Description:''' write here 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ptkA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywqD ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' ||
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 25 kDa, 9.628
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 711 bp, 237 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ptpZ]]'', ''[[tkmA]]''
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ywqD_nucleotide.txt Gene sequence (+200bp) ]'''
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ywqD_protein.txt Protein sequence]'''
|-
|colspan="2" | '''Genetic context''' [[Image:ywqD_context.gif]]
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:'''

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ywqCD.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12508]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' BY-kinase

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:''' autophosphorylation at residues Y225, Y227 and Y228 (primary site)

* '''Cofactor(s):''' ATP

* '''Effectors of protein activity:''' TkmA - transmembrane modulator, activates PtkA autophosphorylation and substrate phosphorylation

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:'''

* '''KEGG entry:'''

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[tkmA]]-[[ptkA]]-[[ptpZ]]-[[ywqF]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/12970183 PubMed]

* '''Sigma factor:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' KO strain created with pMUTIN-2

* '''Expression vector:''' pQE-30, N-terminally 6xHis-tagged

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=
[[Ivan Mijakovic]], Thiverval-Grignon, France

=Your additional remarks=

=References=

# Mijakovic I, Poncet S, Boël G, Mazé A, Gillet S, Decottignies P, Grangeasse C, Jamet E, Doublet P, Le Maréchal P, Deutscher J (2003) Transmembrane modulator-dependent bacterial tyrosine kinase activates UDP-glucose dehydrogenases. EMBO J 22:4709-4718. [http://www.ncbi.nlm.nih.gov/sites/entrez/12970183 PubMed]
# Mijakovic I, Petranovic D, Deutscher J (2004) How tyrosine phosphorylation affects the UDP-glucose dehydrogenase activity of Bacillus subtilis YwqF. J Mol Microbiol Biotechnol 8:19-25. [http://www.ncbi.nlm.nih.gov/sites/entrez/15741737 PubMed]
# Mijakovic I, Petranovic D, Macek B, Cepo T, Mann M, Davies J, Jensen PR, Vujaklija D (2006) Bacterial single-stranded DNA-binding proteins are phosphorylated on tyrosine. Nucl Acids Res 34:1588-1596. [http://www.ncbi.nlm.nih.gov/sites/entrez/16549871 PubMed]
# Petranovic D, Michelsen O, Zahradka K, Silva C, Petranovic M, Jensen PR, Mijakovic I (2007) Bacillus subtilis strain deficient for the protein-tyrosine kinase PtkA exhibits impaired DNA replication. Mol Microbiol 63:1797-1805. [http://www.ncbi.nlm.nih.gov/sites/entrez/17367396 PubMed]

PtkA

2009-03-15T11:22:22Z

Ivanmijakovic:

* '''Description:''' write here 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ptkA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywqD ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' ||
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 25 kDa, 9.628
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 711 bp, 237 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ptpZ]]'', ''[[tkmA]]''
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ywqD_nucleotide.txt Gene sequence (+200bp) ]'''
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ywqD_protein.txt Protein sequence]'''
|-
|colspan="2" | '''Genetic context''' [[Image:ywqD_context.gif]]
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:'''

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ywqCD.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12508]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' BY-kinase

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:''' autophosphorylation at residues Y225, Y227 and Y228 (primary site)

* '''Cofactor(s):''' ATP

* '''Effectors of protein activity:''' TkmA - transmembrane modulator, activates PtkA autophosphorylation and substrate phosphorylation

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:'''

* '''KEGG entry:'''

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[tkmA]]-[[ptkA]]-[[ptpZ]]-[[ywqF]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/12970183 PubMed]

* '''Sigma factor:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' KO strain created with pMUTIN-2

* '''Expression vector:''' pQE-30, N-terminally 6xHis-tagged

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

# Mijakovic I, Poncet S, Boël G, Mazé A, Gillet S, Decottignies P, Grangeasse C, Jamet E, Doublet P, Le Maréchal P, Deutscher J (2003) Transmembrane modulator-dependent bacterial tyrosine kinase activates UDP-glucose dehydrogenases. EMBO J 22:4709-4718. [http://www.ncbi.nlm.nih.gov/sites/entrez/12970183 PubMed]
# Mijakovic I, Petranovic D, Deutscher J (2004) How tyrosine phosphorylation affects the UDP-glucose dehydrogenase activity of Bacillus subtilis YwqF. J Mol Microbiol Biotechnol 8:19-25. [http://www.ncbi.nlm.nih.gov/sites/entrez/15741737 PubMed]
# Mijakovic I, Petranovic D, Macek B, Cepo T, Mann M, Davies J, Jensen PR, Vujaklija D (2006) Bacterial single-stranded DNA-binding proteins are phosphorylated on tyrosine. Nucl Acids Res 34:1588-1596. [http://www.ncbi.nlm.nih.gov/sites/entrez/16549871 PubMed]
# Petranovic D, Michelsen O, Zahradka K, Silva C, Petranovic M, Jensen PR, Mijakovic I (2007) Bacillus subtilis strain deficient for the protein-tyrosine kinase PtkA exhibits impaired DNA replication. Mol Microbiol 63:1797-1805. [http://www.ncbi.nlm.nih.gov/sites/entrez/17367396 PubMed]

PtkA

2009-03-15T11:20:23Z

Ivanmijakovic:

* '''Description:''' write here 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ptkA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywqD ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' ||
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 25 kDa, 9.628
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 711 bp, 237 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ptpZ]]'', ''[[tkmA]]''
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ywqD_nucleotide.txt Gene sequence (+200bp) ]'''
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ywqD_protein.txt Protein sequence]'''
|-
|colspan="2" | '''Genetic context''' [[Image:ywqD_context.gif]]
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Coordinates:'''

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ywqCD.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12508]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' BY-kinase

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:''' autophosphorylation at residues Y225, Y227 and Y228 (primary site)

* '''Cofactor(s):''' ATP

* '''Effectors of protein activity:''' TkmA - transmembrane modulator, activates PtkA autophosphorylation and substrate phosphorylation

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:'''

* '''KEGG entry:'''

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[tkmA]]-[[ptkA]]-[[ptpZ]]-[[ywqF]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/12970183 PubMed]

* '''Sigma factor:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' KO strain created with pMUTIN-2

* '''Expression vector:''' pQE-30, N-terminally 6xHis-tagged

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein= Ivan Mijakovic

=Your additional remarks=

=References=

# Mijakovic I, Poncet S, Boël G, Mazé A, Gillet S, Decottignies P, Grangeasse C, Jamet E, Doublet P, Le Maréchal P, Deutscher J (2003) Transmembrane modulator-dependent bacterial tyrosine kinase activates UDP-glucose dehydrogenases. EMBO J 22:4709-4718. [http://www.ncbi.nlm.nih.gov/sites/entrez/12970183 PubMed]
# Mijakovic I, Petranovic D, Deutscher J (2004) How tyrosine phosphorylation affects the UDP-glucose dehydrogenase activity of Bacillus subtilis YwqF. J Mol Microbiol Biotechnol 8:19-25. [http://www.ncbi.nlm.nih.gov/sites/entrez/15741737 PubMed]
# Mijakovic I, Petranovic D, Macek B, Cepo T, Mann M, Davies J, Jensen PR, Vujaklija D (2006) Bacterial single-stranded DNA-binding proteins are phosphorylated on tyrosine. Nucl Acids Res 34:1588-1596. [http://www.ncbi.nlm.nih.gov/sites/entrez/16549871 PubMed]
# Petranovic D, Michelsen O, Zahradka K, Silva C, Petranovic M, Jensen PR, Mijakovic I (2007) Bacillus subtilis strain deficient for the protein-tyrosine kinase PtkA exhibits impaired DNA replication. Mol Microbiol 63:1797-1805. [http://www.ncbi.nlm.nih.gov/sites/entrez/17367396 PubMed]

# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]