SubtiWiki - User contributions [en]

BglP

2012-10-01T12:06:57Z

Frothe1:

* '''Description:''' [[trigger enzymes|trigger enzyme]]: beta-glucoside-specific [[PTS|phosphotransferase system]], EIIBCA of the [[PTS]] 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''bglP''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''sytA ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || [[trigger enzymes|trigger enzyme]]: beta-glucoside-specific [[PTS|phosphotransferase system]], EIIBCA
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || beta-glucoside uptake and phosphorylation, control of [[LicT]] activity
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU39270 bglP]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/BglP BglP]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 64 kDa, 6.809
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1827 bp, 609 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[bglH]]'', ''[[yxxE]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15963]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:bglP_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bglP_4033778_4035607_-1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:bglP_expression.png|500px]]
|-
|}

__TOC__
 
 
 
 
 

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[phosphotransferase systems]]}},
{{SubtiWiki category|[[utilization of specific carbon sources]]}},
{{SubtiWiki category|[[transcription factors and their control]]}},
{{SubtiWiki category|[[trigger enzyme]]}},
{{SubtiWiki category|[[membrane proteins]]}},
{{SubtiWiki category|[[phosphoproteins]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[CcpA regulon]]}},
{{SubtiWiki regulon|[[LicT regulon]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU39270

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/bglPH-yxiE.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10934]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)

* '''Protein family:''' [[PTS]] permease, sucrose permease (Scr) family [http://www.ncbi.nlm.nih.gov/pubmed/10627040 PubMed]

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''
** [[BglP]]-[[PtsH|HPr]], [[BglP]]-[[LicT]]

* '''[[Localization]]:''' cell membrane (according to Swiss-Prot)

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P40739 P40739]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU39270]

* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.1.69 2.7.1.69] 9

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[bglP]]-[[bglH]]-[[yxiE]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/7883710 PubMed]

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bglP_4033778_4035607_-1 bglP] {{PubMed|22383849}}

* '''Sigma factor:''' [[SigA]] {{PubMed|7559347}}

* '''Regulation:'''
** repressed by glucose ([[CcpA]], [[LicT]]) {{PubMed|7559347}}
** induced by salicin ([[LicT]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/7883710 PubMed]

* '''Regulatory mechanism:'''
** [[CcpA]]: transcription repression, ([[CcpA]] binding site overlaps -35 region) {{PubMed|7559347}}
** [[LicT]]-dependent [[RNA switch]] (antitermination, lack of [[LicT]]-dependent antitermination in the presence of gucose due to the requirement of [[LicT]] to be phosphorylated by [[PtsH| HPr]] {{PubMed|8626332}}

* '''Additional information:'''
** An [[ncRNA|antisense RNA]] is predicted for ''[[bglP]]'' {{PubMed|20525796}}

=Biological materials =

* '''Mutant:''' GP475 (erm), available in the [[Stülke]] lab

* '''Expression vector:'''
** pGP1290 (C-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP382]]), available in [[Stülke]] lab,
** pGP1300 (expression of ''[[bglP]]'' in ''B. subtilis'', in [[pBQ200]]), available in [[Stülke]] lab

* '''lacZ fusion:''' pGP600 (in [[pAC6]]), available in [[Stülke]] lab

* '''GFP fusion:'''

* '''CFP fusion:''' B. subtilis GP1266 bglP-cfp ermC- without terminator, available in [[Jörg Stülke]]'s lab

* '''YFP fusion:''' B. subtilis GP1274 bglP-yfp ermC- without terminator, available in [[Jörg Stülke]]'s lab

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>16672620,20525796,10627040 7883710 8626332 7559347, 22900538 </pubmed>

[[Category:Protein-coding genes]]

PycA

2012-10-01T12:02:27Z

Frothe1:

* '''Description:''' pyruvate carboxylase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''pycA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylaP ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || pyruvate carboxylase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || replenishment of the oxaloacetate pool
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU14860 pycA]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism], [http://subtiwiki.uni-goettingen.de/pathways/biotin/index.html Biotin]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 127 kDa, 5.407
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 3444 bp, 1148 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ftsW]]'', ''[[ctaA]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13359]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:pycA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pycA_1554185_1557631_1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:pycA_expression.png|500px]]
|-
|}

__TOC__
 
 
 
 
 

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[carbon core metabolism]]}},
{{SubtiWiki category|[[membrane proteins]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[stringent response]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU14860

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ftsW-pycA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12660]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):''' biotin

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''

* '''[[Localization]]:''' membrane associated [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3BG5 3BG5] (''S. aureus'')

* '''UniProt:''' [http://www.uniprot.org/uniprot/Q9KWU4 Q9KWU4]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU14860]

* '''E.C. number:''' 6.4.1.1

=== Additional information===
PycA binds to StrepTactin, and may be co-purified when purifying Strep-tagged proteins by [[SPINE]].

PycA is subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]

=Expression and regulation=

* '''Operon:''' ''[[pycA]]'' {{PubMed|20081037}}

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pycA_1554185_1557631_1 pycA] {{PubMed|22383849}}

* '''Sigma factor:''' [[SigA]] {{PubMed|20081037}}

* '''Regulation:'''
** subject to positive stringent control upon lysine starvation {{PubMed|20081037}}

* '''Regulatory mechanism:'''
** stringent response: due to presence of adenines at +1 and +2 positions of the transcript {{PubMed|20081037}}

* '''Additional information:''' subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''
** pGP1289 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
==Reviews==
<pubmed>18613815 12769720 10229653 9597748 7780827 </pubmed>
==Original publications==
<pubmed>18763711, 20081037</pubmed>

[[Category:Protein-coding genes]]

PtsI

2012-10-01T11:54:56Z

Frothe1:

* '''Description:''' Enzyme I, general (non sugar-specific) component of the [[PTS]]. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein ([[PtsH |HPr]]) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ptsI''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || phosphotransferase system ([[PTS]]) enzyme I
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || [[PTS]]-dependent sugar transport
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU13910 ptsI]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PtsI PtsI]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 62,9 kDa, 4.59
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1710 bp, 570 amino acids
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ptsH]]'', ''[[splA]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13264]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:ptsI_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ptsI_1459650_1461362_1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:ptsI_expression.png|500px]]
|-
|}

__TOC__
 
 
 
 
 

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[phosphotransferase systems]]}},
{{SubtiWiki category|[[phosphoproteins]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[GlcT regulon]]}},
{{SubtiWiki regulon|[[stringent response]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU13910

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ptsGHI.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10201]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine (according to Swiss-Prot) PEP-dependent autophosphorylation on His-189, transfer of the phosphoryl group to [[PtsH |HPr]] (His-15)

* '''Protein family:''' PEP-utilizing enzyme family (according to Swiss-Prot) PEP-utilizing enzyme family

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''
**HPr binding site (N-Terminal Domain)
**pyruvate binding site (C-Terminal Domain)
**pyrophosphate/phosphate carrier histidine (central Domain)

* '''Modification:'''
** transient autophosphorylation on His-189
** ''in vivo'' also phosphorylated on Ser-34 or Ser-36 [http://www.ncbi.nlm.nih.gov/pubmed/17218307 PubMed]

* '''Cofactor(s):''' Magnesium

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''

* '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)

=== Database entries ===

* '''Structure:''' [http://www.proteopedia.org/wiki/index.php/2wqd 2WQD] (Enzyme I from ''Staphylococcus aureus'') {{PubMed|19801641}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/P08838 P08838]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU13910]

* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.3.9 2.7.3.9] 2.7.3.9]

=== Additional information===

=Expression and regulation=

* '''Operon:'''
** ''[[ptsG]]-[[ptsH]]-[[ptsI]]'' {{PubMed|11902727}}
**''[[ptsH]]-[[ptsI]]'' {{PubMed|11902727}}

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ptsI_1459650_1461362_1 ptsI] {{PubMed|22383849}}

* '''Sigma factor:'''
** ''[[ptsG]]'': [[SigA]] {{PubMed|11902727}}
** ''[[ptsH]]'': [[SigA]] {{PubMed|11902727}}

* '''Regulation:'''
** expression activated by glucose (2 fold) ([[GlcT]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
** the ''[[ptsH]]'' promoter is constitutive {{PubMed|11902727}}
** subject to negative stringent control upon amino acid limitation (due to control of'' [[ptsG]]'' transcription initiation) {{PubMed|20081037}}

* '''Regulatory mechanism:'''
** ''[[ptsG]]'': transcriptional antitermination via the [[GlcT]]-dependent [[RNA switch]] {{PubMed|9765562}}

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' available in [[Stülke]] lab
** GP864 (ermC)
** GP778 (replacement of ''[[glcT]]'' and the ''[[ptsG]]-[[ptsH]]-[[ptsI]]'' operon by a spc cassette)

* '''Expression vector:'''
** pAG3 (His-tag), available in [[Galinier]] lab
** for expression, purification in ''E. coli'' (His-tag), in [[pWH844]]: pGP813 available in [[Stülke]] lab

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''YFP fusion:''' B. subtilis GP1276 ptsI-yfp ermC, available in [[Jörg Stülke]]'s lab

* '''Antibody:'''

=Labs working on this gene/protein=

[[Josef Deutscher]], Paris-Grignon, France

[[Stülke|Jörg Stülke]], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

=Your additional remarks=

=References=

<pubmed>12850135 17218307, 9721285 20081037 19801641 </pubmed>

[[Category:Protein-coding genes]]

BglP

2012-10-01T11:53:21Z

Frothe1:

* '''Description:''' [[trigger enzymes|trigger enzyme]]: beta-glucoside-specific [[PTS|phosphotransferase system]], EIIBCA of the [[PTS]] 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''bglP''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''sytA ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || [[trigger enzymes|trigger enzyme]]: beta-glucoside-specific [[PTS|phosphotransferase system]], EIIBCA
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || beta-glucoside uptake and phosphorylation, control of [[LicT]] activity
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU39270 bglP]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/BglP BglP]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 64 kDa, 6.809
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1827 bp, 609 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[bglH]]'', ''[[yxxE]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15963]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:bglP_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bglP_4033778_4035607_-1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:bglP_expression.png|500px]]
|-
|}

__TOC__
 
 
 
 
 

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[phosphotransferase systems]]}},
{{SubtiWiki category|[[utilization of specific carbon sources]]}},
{{SubtiWiki category|[[transcription factors and their control]]}},
{{SubtiWiki category|[[trigger enzyme]]}},
{{SubtiWiki category|[[membrane proteins]]}},
{{SubtiWiki category|[[phosphoproteins]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[CcpA regulon]]}},
{{SubtiWiki regulon|[[LicT regulon]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU39270

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/bglPH-yxiE.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10934]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)

* '''Protein family:''' [[PTS]] permease, sucrose permease (Scr) family [http://www.ncbi.nlm.nih.gov/pubmed/10627040 PubMed]

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''
** [[BglP]]-[[PtsH|HPr]], [[BglP]]-[[LicT]]

* '''[[Localization]]:''' cell membrane (according to Swiss-Prot)

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P40739 P40739]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU39270]

* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.1.69 2.7.1.69] 9

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[bglP]]-[[bglH]]-[[yxiE]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/7883710 PubMed]

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bglP_4033778_4035607_-1 bglP] {{PubMed|22383849}}

* '''Sigma factor:''' [[SigA]] {{PubMed|7559347}}

* '''Regulation:'''
** repressed by glucose ([[CcpA]], [[LicT]]) {{PubMed|7559347}}
** induced by salicin ([[LicT]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/7883710 PubMed]

* '''Regulatory mechanism:'''
** [[CcpA]]: transcription repression, ([[CcpA]] binding site overlaps -35 region) {{PubMed|7559347}}
** [[LicT]]-dependent [[RNA switch]] (antitermination, lack of [[LicT]]-dependent antitermination in the presence of gucose due to the requirement of [[LicT]] to be phosphorylated by [[PtsH| HPr]] {{PubMed|8626332}}

* '''Additional information:'''
** An [[ncRNA|antisense RNA]] is predicted for ''[[bglP]]'' {{PubMed|20525796}}

=Biological materials =

* '''Mutant:''' GP475 (erm), available in the [[Stülke]] lab

* '''Expression vector:'''

* '''lacZ fusion:''' pGP600 (in [[pAC6]]), available in [[Stülke]] lab

* '''GFP fusion:'''

* '''CFP fusion:''' B. subtilis GP1266 bglP-cfp ermC- without terminator, available in [[Jörg Stülke]]'s lab

* '''YFP fusion:''' B. subtilis GP1274 bglP-yfp ermC- without terminator, available in [[Jörg Stülke]]'s lab

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>16672620,20525796,10627040 7883710 8626332 7559347, 22900538 </pubmed>

[[Category:Protein-coding genes]]

PtsH

2012-10-01T11:52:24Z

Frothe1:

* '''Description:''' HPr, General component of the sugar phosphotransferase system ([[PTS]]). 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ptsH''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || histidine-containing phosphocarrier protein HPr of the [[PTS]]
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || [[PTS]]-dependent sugar transport and carbon catabolite repression
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU13900 ptsH]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PtsH PtsH]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism], [http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 9,1 kDa, 4.58
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 264 bp, 88 amino acids
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ptsG]]'', ''[[ptsI]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13263]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:ptsH_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ptsH_1459384_1459650_1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:ptsH_expression.png|500px]]
|-
|}

__TOC__
 
 
 
 
 

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[phosphotransferase systems]]}},
{{SubtiWiki category|[[transcription factors and their control]]}},
{{SubtiWiki category|[[phosphoproteins]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[GlcT regulon]]}},
{{SubtiWiki regulon|[[stringent response]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU13900

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ptsGHI.html]

* '''SubtiList entry:'''[http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10200]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' Protein HPr N(pi)-phospho-L-histidine + protein EIIA = protein HPr + protein EIIA N(tau)-phospho-L-histidine (according to Swiss-Prot) Protein HPr N(pi)-phospho-L-histidine + protein EIIA = protein [[PtsH|HPr]] + protein EIIA N(tau)-phospho-L-histidine

* '''Protein family:''' HPr domain (according to Swiss-Prot) HPr family

* '''Paralogous protein(s):''' [[Crh]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:''' HPr Domain (2–88)

* '''Modification:'''
** transient phosphorylation by [[PtsI |Enzyme I]] of the PTS on His-15
** regulatory phosphorylation on Ser-46 by [[HprK]] [http://www.ncbi.nlm.nih.gov/sites/entrez/2507315 PubMed]
** an extensive study on ''in vivo'' HPr phosphorylation can be found in Singh et al. (2008) [http://www.ncbi.nlm.nih.gov/sites/entrez/18757537 PubMed]
** weak phosphorylation on Ser-12 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]
** ''in vitro'' phosphorylated by [[PrkC]] on Ser-12 {{PubMed|20389117}}

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''
** [[HprK]]-[[PtsH|HPr]] [http://www.ncbi.nlm.nih.gov/sites/entrez/12009882 PubMed]
** [[PtsI|Enzyme I]]-[[PtsH|HPr]]
** [[PtsH|HPr]]-[[PtsG]] {{PubMed|8418852}}, [[PtsH]]-[[MtlF]] {{PubMed|20444094}}, [[ManP]]-[[PtsH|HPr]], [[GmuA]]-[[PtsH|HPr]]
** [[GamP]]-[[PtsH|HPr]], [[BglP]]-[[PtsH|HPr]], [[LicA]]-[[PtsH|HPr]], [[LevD]]-[[PtsH|HPr]]
** [[FruA]]-[[PtsH|HPr]], [[YpqE]]-[[PtsH|HPr]]
** [[PtsH|HPr]]-[[LicT]], [[PtsH|HPr]]-[[SacY]], [[PtsH|HPr]]-[[SacT]], [[PtsH|HPr]]-[[GlcT]]
** [[PtsH|HPr]]-[[MtlR]] {{PubMed|20444094}}, [[PtsH|HPr]]-[[LicR]], [[PtsH|HPr]]-[[LevR]],[[PtsH|HPr]]-[[ManR]]
** [[PtsH|HPr]]-[[GlpK]]
** [[GapA]]-[[PtsH|HPr]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17142398 PubMed]
** [[PtsH|HPr(Ser-46)-P]]-[[CcpA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/12432959 PubMed], [[PtsH|HPr]]-[[RbsR]] [http://www.ncbi.nlm.nih.gov/sites/entrez/16519689 PubMed]

** [[PtsH|HPr(His)-P]]-[[YesS]] {{PubMed|19651770}}

* '''[[Localization]]:''' cytoplasm (according to Swiss-Prot), Cytoplasm [http://www.ncbi.nlm.nih.gov/sites/entrez/16395550 PubMed]

=== Database entries ===

* '''Structure:'''
** [http://www.rcsb.org/pdb/explore.do?structureId=1KKM 1KKM] (complex of ''L. casei'' [[HprK]] with ''B. subtilis'' HPr-Ser-P)
** [http://www.rcsb.org/pdb/explore.do?structureId=1KKL 1KKL] (complex of ''Lactobacillus casei'' [[HprK]] with ''B. subtilis'' HPr)
** [http://www.rcsb.org/pdb/explore.do?structureId=2HID 2HID] (NMR)
** [http://www.pdb.org/pdb/search/structidSearch.do?structureId=3OQM 3OQM] (complex of ''B. subtilis'' CcpA with P-Ser-[[PtsH |HPr]] and the ''[[ackA]]'' operator site)
** [http://www.pdb.org/pdb/search/structidSearch.do?structureId=3OQN 3OQN] (complex of ''B. subtilis'' CcpA with P-Ser-[[PtsH |HPr]] and the ''[[gntR]]'' operator site)
** [http://www.pdb.org/pdb/search/structidSearch.do?structureId=3OQO 3OQO] (complex of ''B. subtilis'' CcpA with P-Ser-[[PtsH |HPr]] and a optimal synthetic operator site)
* '''UniProt:''' [http://www.uniprot.org/uniprot/P08877 P08877]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU13900]

* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.11.- 2.7.11.-]

=== Additional information===

=Expression and regulation=

* '''Operon:'''
** ''[[ptsG]]-[[ptsH]]-[[ptsI]]'' {{PubMed|11902727}}
**''[[ptsH]]-[[ptsI]]'' {{PubMed|11902727}}

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ptsH_1459384_1459650_1 ptsH] {{PubMed|22383849}}

* '''Sigma factor:'''
** ''[[ptsG]]'': [[SigA]] {{PubMed|11902727}}
** ''[[ptsH]]'': [[SigA]] {{PubMed|11902727}}

* '''Regulation:'''
** expression activated by glucose (2 fold) ([[GlcT]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
** the ''[[ptsH]]'' promoter is constitutive {{PubMed|11902727}}
** subject to negative stringent control upon amino acid limitation (due to control of'' [[ptsG]]'' transcription initiation) {{PubMed|20081037}}

* '''Regulatory mechanism:'''
** ''[[ptsG]]'': transcriptional antitermination via the [[GlcT]]-dependent [[RNA switch]] {{PubMed|9765562}}

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' available in [[Stülke]] lab
** MZ303 (cat)
** GP507 ptsH1 (S46A)
** GP506 (ptsH-H15A)
** GP778 (replacement of ''[[glcT]]'' and the ''[[ptsG]]-[[ptsH]]-[[ptsI]]'' operon by a spc cassette)

* '''Expression vector:'''
** pGP438 (with N-terminal Strep-tag, in [[pGP172]]), available in [[Stülke]] lab
** pAG2 (His-tag), available in [[Anne Galinier]] lab
** pGP371(expression / purification of HPr-S46A, with His-tag from ''E. coli'', in [[pWH844]]), available in [[Stülke]] lab
** pGP1415 (HPr, expression in ''B. subtilis'', from [[pBQ200]]), available in [[Stülke]] lab
** pGP961 (HPr, expression in ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], available in [[Stülke]] lab
** pGP1416 (HPr-H15A, expression in ''B. subtilis'', from [[pBQ200]]), available in [[Stülke]] lab

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''CFP fusion:''' B. subtilis GP1267 ptsH-cfp ermC- without terminator, available in [[Jörg Stülke]]'s lab

* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Boris Görke| Görke]] lab

* '''Antibody:''' available in [[Stülke]] lab

=Labs working on this gene/protein=

[[Josef Deutscher]], Paris-Grignon, France

[[Stülke|Jörg Stülke]], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

[[Wolfgang Hillen]], Erlangen University, Germany [http://www.biologie.uni-erlangen.de/mibi/index2.html Homepage]

[[Richard Brennan]], Houston, Texas, USA [http://www.mdanderson.org/departments/biochem/display.cfm?id=556ef368-6c81-4043-b74f350d41dd06cb&method=displayfull&pn=a8427ebd-d0ff-11d4-80fd00508b603a14 Homepage]

[[Boris Görke]], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/goerke.html Homepage]

[[Anne Galinier]], University of Marseille, France

=Your additional remarks=

=References=

<pubmed>16519689,,12850135 17218307 16519689 17142398 12359875 1577686 9162046 11929549 9336834 7803390, 7623661, 2846556, 8169206, 9973552, 15126459, 10048041, 12169607, 9622354, 10217795, 17693724, 18757537 , 9202047 , 7592487, 15369672 , 14527945, 2507315 8580838 19651770 8418852 1303754 1549615 20081037 20389117 20444094 22001508 22722928</pubmed>

[[Category:Protein-coding genes]]

BglP

2012-10-01T11:49:11Z

Frothe1:

* '''Description:''' [[trigger enzymes|trigger enzyme]]: beta-glucoside-specific [[PTS|phosphotransferase system]], EIIBCA of the [[PTS]] 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''bglP''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''sytA ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || [[trigger enzymes|trigger enzyme]]: beta-glucoside-specific [[PTS|phosphotransferase system]], EIIBCA
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || beta-glucoside uptake and phosphorylation, control of [[LicT]] activity
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU39270 bglP]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/BglP BglP]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 64 kDa, 6.809
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1827 bp, 609 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[bglH]]'', ''[[yxxE]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15963]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:bglP_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bglP_4033778_4035607_-1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:bglP_expression.png|500px]]
|-
|}

__TOC__
 
 
 
 
 

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[phosphotransferase systems]]}},
{{SubtiWiki category|[[utilization of specific carbon sources]]}},
{{SubtiWiki category|[[transcription factors and their control]]}},
{{SubtiWiki category|[[trigger enzyme]]}},
{{SubtiWiki category|[[membrane proteins]]}},
{{SubtiWiki category|[[phosphoproteins]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[CcpA regulon]]}},
{{SubtiWiki regulon|[[LicT regulon]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU39270

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/bglPH-yxiE.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10934]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)

* '''Protein family:''' [[PTS]] permease, sucrose permease (Scr) family [http://www.ncbi.nlm.nih.gov/pubmed/10627040 PubMed]

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''
** [[BglP]]-[[PtsH|HPr]], [[BglP]]-[[LicT]]

* '''[[Localization]]:''' cell membrane (according to Swiss-Prot)

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P40739 P40739]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU39270]

* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.1.69 2.7.1.69] 9

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[bglP]]-[[bglH]]-[[yxiE]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/7883710 PubMed]

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bglP_4033778_4035607_-1 bglP] {{PubMed|22383849}}

* '''Sigma factor:''' [[SigA]] {{PubMed|7559347}}

* '''Regulation:'''
** repressed by glucose ([[CcpA]], [[LicT]]) {{PubMed|7559347}}
** induced by salicin ([[LicT]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/7883710 PubMed]

* '''Regulatory mechanism:'''
** [[CcpA]]: transcription repression, ([[CcpA]] binding site overlaps -35 region) {{PubMed|7559347}}
** [[LicT]]-dependent [[RNA switch]] (antitermination, lack of [[LicT]]-dependent antitermination in the presence of gucose due to the requirement of [[LicT]] to be phosphorylated by [[PtsH| HPr]] {{PubMed|8626332}}

* '''Additional information:'''
** An [[ncRNA|antisense RNA]] is predicted for ''[[bglP]]'' {{PubMed|20525796}}

=Biological materials =

* '''Mutant:''' GP475 (erm), available in the [[Stülke]] lab

* '''Expression vector:'''

* '''lacZ fusion:''' pGP600 (in [[pAC6]]), available in [[Stülke]] lab

* '''GFP fusion:'''

* '''CFP fusion:''' B. subtilis GP1266 bglP-cfp ermC- without terminator, available in [[Jörg Stülke]]'s lab

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>16672620,20525796,10627040 7883710 8626332 7559347, 22900538 </pubmed>

[[Category:Protein-coding genes]]

BglP

2012-10-01T11:47:23Z

Frothe1:

* '''Description:''' [[trigger enzymes|trigger enzyme]]: beta-glucoside-specific [[PTS|phosphotransferase system]], EIIBCA of the [[PTS]] 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''bglP''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''sytA ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || [[trigger enzymes|trigger enzyme]]: beta-glucoside-specific [[PTS|phosphotransferase system]], EIIBCA
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || beta-glucoside uptake and phosphorylation, control of [[LicT]] activity
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU39270 bglP]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/BglP BglP]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 64 kDa, 6.809
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1827 bp, 609 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[bglH]]'', ''[[yxxE]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15963]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:bglP_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bglP_4033778_4035607_-1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:bglP_expression.png|500px]]
|-
|}

__TOC__
 
 
 
 
 

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[phosphotransferase systems]]}},
{{SubtiWiki category|[[utilization of specific carbon sources]]}},
{{SubtiWiki category|[[transcription factors and their control]]}},
{{SubtiWiki category|[[trigger enzyme]]}},
{{SubtiWiki category|[[membrane proteins]]}},
{{SubtiWiki category|[[phosphoproteins]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[CcpA regulon]]}},
{{SubtiWiki regulon|[[LicT regulon]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU39270

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/bglPH-yxiE.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10934]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)

* '''Protein family:''' [[PTS]] permease, sucrose permease (Scr) family [http://www.ncbi.nlm.nih.gov/pubmed/10627040 PubMed]

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''
** [[BglP]]-[[PtsH|HPr]], [[BglP]]-[[LicT]]

* '''[[Localization]]:''' cell membrane (according to Swiss-Prot)

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P40739 P40739]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU39270]

* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.1.69 2.7.1.69] 9

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[bglP]]-[[bglH]]-[[yxiE]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/7883710 PubMed]

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=bglP_4033778_4035607_-1 bglP] {{PubMed|22383849}}

* '''Sigma factor:''' [[SigA]] {{PubMed|7559347}}

* '''Regulation:'''
** repressed by glucose ([[CcpA]], [[LicT]]) {{PubMed|7559347}}
** induced by salicin ([[LicT]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/7883710 PubMed]

* '''Regulatory mechanism:'''
** [[CcpA]]: transcription repression, ([[CcpA]] binding site overlaps -35 region) {{PubMed|7559347}}
** [[LicT]]-dependent [[RNA switch]] (antitermination, lack of [[LicT]]-dependent antitermination in the presence of gucose due to the requirement of [[LicT]] to be phosphorylated by [[PtsH| HPr]] {{PubMed|8626332}}

* '''Additional information:'''
** An [[ncRNA|antisense RNA]] is predicted for ''[[bglP]]'' {{PubMed|20525796}}

=Biological materials =

* '''Mutant:''' GP475 (erm), available in the [[Stülke]] lab

* '''Expression vector:'''

* '''lacZ fusion:''' pGP600 (in [[pAC6]]), available in [[Stülke]] lab

* '''GFP fusion:'''

* '''CFP fusion:'''
** ''B. subtilis'' GP1266 bglP-cfp ermC-[without terminator], available in [[Jörg Stülke]]'s lab,

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>16672620,20525796,10627040 7883710 8626332 7559347, 22900538 </pubmed>

[[Category:Protein-coding genes]]

GlcT

2012-09-28T13:36:43Z

Frothe1:

* '''Description:''' [[PRD-containing transcription factors|transcriptional antiterminator]] , controls expression of the ''[[ptsG]]-[[ptsH]]-[[ptsI]]'' operon 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''glcT''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ykwA''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || [[PRD-containing transcription factors|transcriptional antiterminator]] of the ''[[ptsG]]-[[ptsH]]-[[ptsI]]'' operon
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || control of glucose uptake
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU13880 glcT]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/GlcT GlcT]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33,0 kDa, 7.01
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 855 bp, 285 amino acids
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykvZ]]'', ''[[ptsG]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13261]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:glcT_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glcT_1456092_1456958_1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:glcT_expression.png|500px]]
|-
|}

__TOC__
 
 
 
 
 

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[carbon core metabolism]]}},
{{SubtiWiki category|[[transcription factors and their control]]}},
{{SubtiWiki category|[[RNA binding regulators]]}},
{{SubtiWiki category|[[phosphoproteins]]}}

= This gene is a member of the following [[regulons]] =
=The [[GlcT regulon]]: ''[[ptsG]]-[[ptsH]]-[[ptsI]]''=

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU13880

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12593]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' [[PRD-containing transcription factors|transcription antiterminator]] , RNA-binding protein, binds the ''[[ptsG]]'' RAT sequence

* '''Protein family:''' [[PRD-containing transcription factors|transcription antiterminator]] of the BglG/ [[SacY]] family

* '''Paralogous protein(s):''' [[LicT]], [[SacT]], [[SacY]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''
** RNA-binding domain (N-terminal, constitutive antiterminator)
** 2x [[PTS]] regulation domains (PRDs) (C-terminal, neg. regulated by [[PtsG]])

* '''Modification:''' phosphorylation (His104)

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''
** [[PtsH]]-[[GlcT]]

* '''[[Localization]]:'''

=== Database entries ===

* '''Structure:'''
** [http://www.rcsb.org/pdb/search/structidSearch.do?structureId=3GWH 3GWH] (PRD-II) {{PubMed|19684596}}
** [http://www.rcsb.org/pdb/explore.do?structureId=3rio 3RIO] (RBD-PRD-I) {{PubMed|22750856}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/O31691 O31691]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU13880]

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glcT_1456092_1456958_1 glcT] {{PubMed|22383849}}

* '''Sigma factor:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' available in [[Stülke]] lab:
** GP109 (in frame deletion)
** GP778 (replacement of ''[[glcT]]'' and the ''[[ptsG]]-[[ptsH]]-[[ptsI]]'' operon by a spc cassette)
** GP926 (substitution of ''[[glcT]]'' and ''[[ptsG]]'' by a tet cassette)

* '''Expression vector:'''
** pGP124 (full length, in [[pWH844]]), available in [[Stülke]] lab
** pGP114 (amino acids 1-60, RNA-binding domain, in [[pWH844]]), available in [[Stülke]] lab
** pGP230 (amino acids 1-60, RNA-binding domain with thrombin cleavage site, in [[pWH844]]), available in [[Stülke]] lab
** pGP164 (both PRDs, in [[pWH844]]), in addition diverse expression vectors for phosphorylation site mutants and for RBD mutants (all in [[pWH844]]), available in [[Stülke]] lab
** pGP424 (PRDI, in [[pWH844]]), available in [[Stülke]] lab
** pGP425 (PRDII, in [[pWH844]]), available in [[Stülke]] lab
** pGP442 (PRDI, in [[pGP570]], with thrombin cleavage site), available in [[Stülke]] lab
** pGP443 (PRDII, in [[pGP570]], with thrombin cleavage site), available in [[Stülke]] lab
** pGP575 (amino acids 1-60, RNA-binding domain with Strep-tag, in [[pGP574]]), available in [[Stülke]] lab

* '''lacZ fusion:'''

* '''GFP fusion:''' GP1224 (spc, based on [[pGP1870]]), available in the [[Stülke]] lab

* '''YFP fusion:''' GP1228 (spc, based on [[pGP1871]]), available in the [[Stülke]] lab

* '''FLAG-tag construct:''' GP1220 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab

* '''Antibody:'''

=Labs working on this gene/protein=

[[Stülke|Jörg Stülke]], University of Göttingen, Germany
[http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

=Your additional remarks=

=References=
==Reviews==
<pubmed> 9663674 18086213 </pubmed>
==Original publications==
'''Additional publications:''' {{PubMed|22750856}}
<pubmed>11902727 9765562 12437213 10543968 17074746 15155854 14527945 , 19684596 22722928 20939030 </pubmed>
[[Category:Protein-coding genes]]

SacT

2012-09-28T13:35:41Z

Frothe1:

* '''Description:''' [[PRD-containing transcription factors|transcriptional antiterminator]] for the ''[[sacP]]-[[sacA]]-[[ywdA]]'' operon 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''sacT''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipa-47d ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || [[PRD-containing transcription factors|transcriptional antiterminator]]
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of sucrose utilization
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU38070 sacT]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SacT SacT]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism], [http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 5.587
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 828 bp, 276 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywcJ]]'', ''[[ywcI]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15833]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:sacT_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sacT_3906142_3906972_-1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:sacT_expression.png|500px]]
|-
|}

__TOC__
 
 
 
 
 

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[utilization of specific carbon sources]]}},
{{SubtiWiki category|[[transcription factors and their control]]}},
{{SubtiWiki category|[[RNA binding regulators]]}},
{{SubtiWiki category|[[phosphoproteins]]}}

= This gene is a member of the following [[regulons]] =

=The [[SacT regulon]]: ''[[sacP]]-[[sacA]]-[[ywdA]]''=
=The gene=

=== Basic information ===

* '''Locus tag:''' BSU38070

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sacT.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10593]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' binding to the mRNA of the ''[[sacP]]-[[sacA]]'' operon, causes transcription antitermination (in presence of sucrose and absence of glucose)

* '''Protein family:''' [[PRD-containing transcription factors|transcriptional antiterminator]] bglG family (according to Swiss-Prot) BglG family of antiterminators

* '''Paralogous protein(s):''' [[LicT]], [[SacY]], [[GlcT]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''
** [[PtsH]]-[[SacT]]

* '''[[Localization]]:'''

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P26212 P26212]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU38070]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[sacT]]'' {{PubMed|9353933}}

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sacT_3906142_3906972_-1 sacT] {{PubMed|22383849}}

* '''Sigma factor:'''

* '''Regulation:'''
** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' GP429 (spc), available in [[Stülke]] lab

* '''Expression vector:'''
** for expression, purification of both PRDs in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP166, available in [[Stülke]] lab
** for expression, purification of PRD-1 in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP426, available in [[Stülke]] lab
** for expression, purification of PRD-2 in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP427, available in [[Stülke]] lab
** for expression, purification of PRD-1 in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [[pGP570]]: pGP439, available in [[Stülke]] lab
** for expression, purification of PRD-2 in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [[pGP570]]: pGP440, available in [[Stülke]] lab
** for expression, purification of the RNA-binding domain in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [[pGP570]]: pGP571, available in [[Stülke]] lab
** for expression of the RNA-binding domain in ''B. subtilis'', in [[pBQ200]]: pGP446, available in [[Stülke]] lab
** for expression, purification of [[sacT]]-full length in ''B. subtilis'' with C-terminal Strep-tag, in [[pGP382]]: pGP1064, available in [[Stülke]] lab
** for expression, purification of [[sacT]]-full length in ''B. subtilis'' with N-terminal Strep-tag, in [[pGP380]]: pGP1068, available in [[Stülke]] lab

* '''lacZ fusion:'''

* '''GFP fusion:''' GP1227 (spc, based on [[pGP1870]]), available in the [[Stülke]] lab

* '''YFP fusion:''' GP1231 (spc, based on [[pGP1871]]), available in the [[Stülke]] lab

* '''FLAG-tag construct:''' GP1223 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>12107147 2163394 1577686 8702561 1279678, 21278164 </pubmed>

[[Category:Protein-coding genes]]

SacY

2012-09-28T13:34:42Z

Frothe1:

* '''Description:''' [[PRD-containing transcription factors|transcriptional antiterminator]] for ''[[sacB]]'' and ''[[sacX]]-[[sacY]]'' (acts at high sucrose concentrations) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''sacY''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipa-13r, sacS ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || [[PRD-containing transcription factors|transcriptional antiterminator]]
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of sucrose utilization
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU38420 sacY]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SacY SacY]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism], [http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 5.883
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 840 bp, 280 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sacX]]'', ''[[gspA]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15868]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:sacY_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sacY_3943667_3944509_1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:sacY_expression.png|500px]]
|-
|}

__TOC__
 
 
 
 
 

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[utilization of specific carbon sources]]}},
{{SubtiWiki category|[[transcription factors and their control]]}},
{{SubtiWiki category|[[RNA binding regulators]]}},
{{SubtiWiki category|[[phosphoproteins]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[SacY regulon]]}}

=The [[SacY regulon]]: ''[[sacB]]-[[levB]]-[[yveA]]'', ''[[sacX]]-[[sacY]]''=

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU38420

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sacXY.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10559]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' binding to the mRNA of'' [[sacB]]'' and the ''[[sacX]]-[[sacY]]'' operon, causes transcription antitermination (in presence of sucrose)

* '''Protein family:''' [[PRD-containing transcription factors|transcriptional antiterminator]] bglG family (according to Swiss-Prot) BglG family of antiterminators

* '''Paralogous protein(s):''' [[LicT]], [[GlcT]], [[SacT]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''
** [[PtsH]]-[[SacY]]

* '''[[Localization]]:'''

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1AUU 1AUU] (RNA-binding domain)

* '''UniProt:''' [http://www.uniprot.org/uniprot/P15401 P15401]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU38420]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=
* '''Operon:''' ''[[sacX]]-[[sacY]]'' {{PubMed|2116367}}

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sacY_3943667_3944509_1 sacY] {{PubMed|22383849}}

* '''Sigma factor:''' [[SigA]] {{PubMed|1400159}}

* '''Regulation:''' induction by sucrose (at high concentration) {{PubMed|8535520}}

* '''Regulatory mechanism:'''
** induction by [[SacY]]-dependent [[RNA switch]] (transcriptional antitermination) {{PubMed|8535520}}
** [[DegU]]-P: transcription activation {{PubMed|1400159}}

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' GP425 (cat), available in [[Stülke]] lab

* '''Expression vector:'''
** for expression, purification of the RNA-binding domain in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP316, available in [[Stülke]] lab
** for expression, purification of the RNA-binding domain in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [[pGP570]]: pGP573, available in [[Stülke]] lab

* '''lacZ fusion:'''

* '''GFP fusion:''' GP1226 (spc, based on [[pGP1870]]), available in the [[Stülke]] lab

* '''YFP fusion:''' GP1230 (spc, based on [[pGP1871]]), available in the [[Stülke]] lab

* '''FLAG-tag construct:''' GP1222 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Stephane Aymerich |Stephane Aymerich]], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

=Your additional remarks=

=References=

<pubmed>2105292 8702561 11580842 1279678 9305643 9305644 9202047 10610766,2116367 1400159 8535520 21278164 </pubmed>

[[Category:Protein-coding genes]]

LicT

2012-09-28T13:33:17Z

Frothe1:

* '''Description:''' [[PRD-containing transcription factors|transcriptional antiterminator]] of the ''[[bglP]]-[[bglH]]-[[yxiE]]'' operon and the ''[[bglS]]'' gene 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''licT''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || [[PRD-containing transcription factors|transcriptional antiterminator]] (BglG family)
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || control of beta-glucan and beta-glucoside utilization
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU39080 licT]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/LicT LicT]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 5.944
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 831 bp, 277 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[bglS]]'', ''[[yxiP]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15944]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:licT_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=licT_4012866_4013699_-1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:licT_expression.png|500px]]
|-
|}

__TOC__
 
 
 
 
 

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[utilization of specific carbon sources]]}},
{{SubtiWiki category|[[transcription factors and their control]]}},
{{SubtiWiki category|[[RNA binding regulators]]}},
{{SubtiWiki category|[[phosphoproteins]]}}

= This gene is a member of the following [[regulons]] =

=The [[LicT regulon]]: ''[[bglP]]-[[bglH]]-[[yxiE]]'', ''[[bglS]]''=
=The gene=

=== Basic information ===

* '''Locus tag:''' BSU39080

===Phenotypes of a mutant ===

no expression of the ''[[bglP]]-[[bglH]]'' operon

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/licT-bglS.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10474]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' binding to the mRNAs of ''[[bglS]]'' and the ''[[bglP]]-[[bglH]]'' operon, causes transcription antitermination (in presence of salicin and absence of glucose)

* '''Protein family:''' [[PRD-containing transcription factors|transcriptional antiterminator]] BglG family of antiterminators (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[SacY]], [[GlcT]], [[SacT]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''
** N-terminal RNA binding domain [http://www.ncbi.nlm.nih.gov/pubmed/10610766?dopt=Abstract Pubmed]
** 2xPRD ([[PTS]] regulation domains) [http://www.ncbi.nlm.nih.gov/pubmed/11447120?dopt=Abstract PubMed]

* '''Modification:'''
** phosphorylation at His-100 in PRD-1 by phosphorylated [[BglP]], inhibits LicT antitermination activity
** phosphorylation at His-207 and/or His-269 in PRD-2 by His-P-[[ptsH|HPr]], stimulates LicT antitermination activity

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:''' [[PtsH]]-[[LicT]], [[BglP]]-[[LicT]]

* '''[[Localization]]:'''

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1L1C 1L1C] (complex with RAT), [http://www.rcsb.org/pdb/explore.do?structureId=1TLV 1TLV] (PRDs)

* '''UniProt:''' [http://www.uniprot.org/uniprot/P39805 P39805]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU39080]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[licT]]-[[bglS]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/8606172 PubMed]

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=licT_4012866_4013699_-1 licT] {{PubMed|22383849}}

* '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/8606172 PubMed]

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' GP427 (licTS, erm), available in the [[Stülke]] lab

* '''Expression vector:'''

** for expression, purification of both PRDs in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP165, available in [[Stülke]] lab
** for expression, purification of the RNA-binding domain in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP315, available in [[Stülke]] lab
** for expression, purification of the RNA-binding domain in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [[pGP570]]: pGP572, available in [[Stülke]] lab

* '''lacZ fusion:'''

* '''GFP fusion:''' GP1225 (spc, based on [[pGP1870]]), available in the [[Stülke]] lab

* '''YFP fusion:''' GP1229 (spc, based on [[pGP1871]]), available in the [[Stülke]] lab

* '''FLAG-tag construct:''' GP1221 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Stephane Aymerich |Stephane Aymerich]], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

[[Josef Deutscher]], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

[[Michael Hecker]], Greifswald, Germany [http://www.mikrobiologie.uni-greifswald.de/index.php?id=20 Homepage]

=Your additional remarks=

=References=
'''Additional publications:''' {{PubMed|21335451}}

'''Original description'''
<pubmed>8606172 </pubmed>

'''Control of LicT activity'''
<pubmed>10048041, 12169607,8626332, 11580842 </pubmed>

'''Structural analysis of LicT'''
<pubmed>11733988,15699035,11447120,18682383 </pubmed>

'''LicT-RNA interaction'''
<pubmed>11953318,1279678, 10610766,18682383 21278164 </pubmed>

[[Category:Protein-coding genes]]

SacT

2012-09-28T13:23:35Z

Frothe1:

* '''Description:''' [[PRD-containing transcription factors|transcriptional antiterminator]] for the ''[[sacP]]-[[sacA]]-[[ywdA]]'' operon 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''sacT''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipa-47d ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || [[PRD-containing transcription factors|transcriptional antiterminator]]
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of sucrose utilization
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU38070 sacT]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SacT SacT]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism], [http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 5.587
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 828 bp, 276 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywcJ]]'', ''[[ywcI]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15833]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:sacT_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sacT_3906142_3906972_-1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:sacT_expression.png|500px]]
|-
|}

__TOC__
 
 
 
 
 

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[utilization of specific carbon sources]]}},
{{SubtiWiki category|[[transcription factors and their control]]}},
{{SubtiWiki category|[[RNA binding regulators]]}},
{{SubtiWiki category|[[phosphoproteins]]}}

= This gene is a member of the following [[regulons]] =

=The [[SacT regulon]]: ''[[sacP]]-[[sacA]]-[[ywdA]]''=
=The gene=

=== Basic information ===

* '''Locus tag:''' BSU38070

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sacT.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10593]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' binding to the mRNA of the ''[[sacP]]-[[sacA]]'' operon, causes transcription antitermination (in presence of sucrose and absence of glucose)

* '''Protein family:''' [[PRD-containing transcription factors|transcriptional antiterminator]] bglG family (according to Swiss-Prot) BglG family of antiterminators

* '''Paralogous protein(s):''' [[LicT]], [[SacY]], [[GlcT]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''
** [[PtsH]]-[[SacT]]

* '''[[Localization]]:'''

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P26212 P26212]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU38070]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[sacT]]'' {{PubMed|9353933}}

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sacT_3906142_3906972_-1 sacT] {{PubMed|22383849}}

* '''Sigma factor:'''

* '''Regulation:'''
** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' GP429 (spc), available in [[Stülke]] lab

* '''Expression vector:'''
** for expression, purification of both PRDs in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP166, available in [[Stülke]] lab
** for expression, purification of PRD-1 in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP426, available in [[Stülke]] lab
** for expression, purification of PRD-2 in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP427, available in [[Stülke]] lab
** for expression, purification of PRD-1 in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [[pGP570]]: pGP439, available in [[Stülke]] lab
** for expression, purification of PRD-2 in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [[pGP570]]: pGP440, available in [[Stülke]] lab
** for expression, purification of the RNA-binding domain in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [[pGP570]]: pGP571, available in [[Stülke]] lab
** for expression of the RNA-binding domain in ''B. subtilis'', in [[pBQ200]]: pGP446, available in [[Stülke]] lab
** for expression, purification of [[sacT]]-full length in ''B. subtilis'' with C-terminal Strep-tag, in [[pGP382]]: pGP1064, available in [[Stülke]] lab
** for expression, purification of [[sacT]]-full length in ''B. subtilis'' with N-terminal Strep-tag, in [[pGP380]]: pGP1068, available in [[Stülke]] lab

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''FLAG-tag construct:''' GP1223 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>12107147 2163394 1577686 8702561 1279678, 21278164 </pubmed>

[[Category:Protein-coding genes]]

SacY

2012-09-28T13:22:53Z

Frothe1:

* '''Description:''' [[PRD-containing transcription factors|transcriptional antiterminator]] for ''[[sacB]]'' and ''[[sacX]]-[[sacY]]'' (acts at high sucrose concentrations) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''sacY''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipa-13r, sacS ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || [[PRD-containing transcription factors|transcriptional antiterminator]]
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of sucrose utilization
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU38420 sacY]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SacY SacY]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism], [http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 5.883
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 840 bp, 280 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sacX]]'', ''[[gspA]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15868]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:sacY_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sacY_3943667_3944509_1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:sacY_expression.png|500px]]
|-
|}

__TOC__
 
 
 
 
 

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[utilization of specific carbon sources]]}},
{{SubtiWiki category|[[transcription factors and their control]]}},
{{SubtiWiki category|[[RNA binding regulators]]}},
{{SubtiWiki category|[[phosphoproteins]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[SacY regulon]]}}

=The [[SacY regulon]]: ''[[sacB]]-[[levB]]-[[yveA]]'', ''[[sacX]]-[[sacY]]''=

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU38420

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sacXY.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10559]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' binding to the mRNA of'' [[sacB]]'' and the ''[[sacX]]-[[sacY]]'' operon, causes transcription antitermination (in presence of sucrose)

* '''Protein family:''' [[PRD-containing transcription factors|transcriptional antiterminator]] bglG family (according to Swiss-Prot) BglG family of antiterminators

* '''Paralogous protein(s):''' [[LicT]], [[GlcT]], [[SacT]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''
** [[PtsH]]-[[SacY]]

* '''[[Localization]]:'''

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1AUU 1AUU] (RNA-binding domain)

* '''UniProt:''' [http://www.uniprot.org/uniprot/P15401 P15401]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU38420]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=
* '''Operon:''' ''[[sacX]]-[[sacY]]'' {{PubMed|2116367}}

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sacY_3943667_3944509_1 sacY] {{PubMed|22383849}}

* '''Sigma factor:''' [[SigA]] {{PubMed|1400159}}

* '''Regulation:''' induction by sucrose (at high concentration) {{PubMed|8535520}}

* '''Regulatory mechanism:'''
** induction by [[SacY]]-dependent [[RNA switch]] (transcriptional antitermination) {{PubMed|8535520}}
** [[DegU]]-P: transcription activation {{PubMed|1400159}}

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' GP425 (cat), available in [[Stülke]] lab

* '''Expression vector:'''
** for expression, purification of the RNA-binding domain in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP316, available in [[Stülke]] lab
** for expression, purification of the RNA-binding domain in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [[pGP570]]: pGP573, available in [[Stülke]] lab

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''FLAG-tag construct:''' GP1222 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Stephane Aymerich |Stephane Aymerich]], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

=Your additional remarks=

=References=

<pubmed>2105292 8702561 11580842 1279678 9305643 9305644 9202047 10610766,2116367 1400159 8535520 21278164 </pubmed>

[[Category:Protein-coding genes]]

LicT

2012-09-28T13:22:44Z

Frothe1:

* '''Description:''' [[PRD-containing transcription factors|transcriptional antiterminator]] of the ''[[bglP]]-[[bglH]]-[[yxiE]]'' operon and the ''[[bglS]]'' gene 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''licT''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || [[PRD-containing transcription factors|transcriptional antiterminator]] (BglG family)
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || control of beta-glucan and beta-glucoside utilization
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU39080 licT]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/LicT LicT]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 5.944
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 831 bp, 277 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[bglS]]'', ''[[yxiP]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15944]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' [[Image:licT_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=licT_4012866_4013699_-1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:licT_expression.png|500px]]
|-
|}

__TOC__
 
 
 
 
 

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[utilization of specific carbon sources]]}},
{{SubtiWiki category|[[transcription factors and their control]]}},
{{SubtiWiki category|[[RNA binding regulators]]}},
{{SubtiWiki category|[[phosphoproteins]]}}

= This gene is a member of the following [[regulons]] =

=The [[LicT regulon]]: ''[[bglP]]-[[bglH]]-[[yxiE]]'', ''[[bglS]]''=
=The gene=

=== Basic information ===

* '''Locus tag:''' BSU39080

===Phenotypes of a mutant ===

no expression of the ''[[bglP]]-[[bglH]]'' operon

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/licT-bglS.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10474]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' binding to the mRNAs of ''[[bglS]]'' and the ''[[bglP]]-[[bglH]]'' operon, causes transcription antitermination (in presence of salicin and absence of glucose)

* '''Protein family:''' [[PRD-containing transcription factors|transcriptional antiterminator]] BglG family of antiterminators (according to Swiss-Prot)

* '''Paralogous protein(s):''' [[SacY]], [[GlcT]], [[SacT]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''
** N-terminal RNA binding domain [http://www.ncbi.nlm.nih.gov/pubmed/10610766?dopt=Abstract Pubmed]
** 2xPRD ([[PTS]] regulation domains) [http://www.ncbi.nlm.nih.gov/pubmed/11447120?dopt=Abstract PubMed]

* '''Modification:'''
** phosphorylation at His-100 in PRD-1 by phosphorylated [[BglP]], inhibits LicT antitermination activity
** phosphorylation at His-207 and/or His-269 in PRD-2 by His-P-[[ptsH|HPr]], stimulates LicT antitermination activity

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:''' [[PtsH]]-[[LicT]], [[BglP]]-[[LicT]]

* '''[[Localization]]:'''

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1L1C 1L1C] (complex with RAT), [http://www.rcsb.org/pdb/explore.do?structureId=1TLV 1TLV] (PRDs)

* '''UniProt:''' [http://www.uniprot.org/uniprot/P39805 P39805]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU39080]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[licT]]-[[bglS]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/8606172 PubMed]

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=licT_4012866_4013699_-1 licT] {{PubMed|22383849}}

* '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/8606172 PubMed]

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' GP427 (licTS, erm), available in the [[Stülke]] lab

* '''Expression vector:'''

** for expression, purification of both PRDs in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP165, available in [[Stülke]] lab
** for expression, purification of the RNA-binding domain in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP315, available in [[Stülke]] lab
** for expression, purification of the RNA-binding domain in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [[pGP570]]: pGP572, available in [[Stülke]] lab

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''FLAG-tag construct:''' GP1221 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Stephane Aymerich |Stephane Aymerich]], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

[[Josef Deutscher]], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

[[Michael Hecker]], Greifswald, Germany [http://www.mikrobiologie.uni-greifswald.de/index.php?id=20 Homepage]

=Your additional remarks=

=References=
'''Additional publications:''' {{PubMed|21335451}}

'''Original description'''
<pubmed>8606172 </pubmed>

'''Control of LicT activity'''
<pubmed>10048041, 12169607,8626332, 11580842 </pubmed>

'''Structural analysis of LicT'''
<pubmed>11733988,15699035,11447120,18682383 </pubmed>

'''LicT-RNA interaction'''
<pubmed>11953318,1279678, 10610766,18682383 21278164 </pubmed>

[[Category:Protein-coding genes]]

GlcT

2012-09-28T13:19:53Z

Frothe1:

* '''Description:''' [[PRD-containing transcription factors|transcriptional antiterminator]] , controls expression of the ''[[ptsG]]-[[ptsH]]-[[ptsI]]'' operon 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''glcT''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ykwA''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || [[PRD-containing transcription factors|transcriptional antiterminator]] of the ''[[ptsG]]-[[ptsH]]-[[ptsI]]'' operon
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || control of glucose uptake
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU13880 glcT]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/GlcT GlcT]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33,0 kDa, 7.01
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 855 bp, 285 amino acids
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykvZ]]'', ''[[ptsG]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13261]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:glcT_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glcT_1456092_1456958_1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:glcT_expression.png|500px]]
|-
|}

__TOC__
 
 
 
 
 

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[carbon core metabolism]]}},
{{SubtiWiki category|[[transcription factors and their control]]}},
{{SubtiWiki category|[[RNA binding regulators]]}},
{{SubtiWiki category|[[phosphoproteins]]}}

= This gene is a member of the following [[regulons]] =
=The [[GlcT regulon]]: ''[[ptsG]]-[[ptsH]]-[[ptsI]]''=

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU13880

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12593]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' [[PRD-containing transcription factors|transcription antiterminator]] , RNA-binding protein, binds the ''[[ptsG]]'' RAT sequence

* '''Protein family:''' [[PRD-containing transcription factors|transcription antiterminator]] of the BglG/ [[SacY]] family

* '''Paralogous protein(s):''' [[LicT]], [[SacT]], [[SacY]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''
** RNA-binding domain (N-terminal, constitutive antiterminator)
** 2x [[PTS]] regulation domains (PRDs) (C-terminal, neg. regulated by [[PtsG]])

* '''Modification:''' phosphorylation (His104)

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''[[SubtInteract|Interactions]]:'''
** [[PtsH]]-[[GlcT]]

* '''[[Localization]]:'''

=== Database entries ===

* '''Structure:'''
** [http://www.rcsb.org/pdb/search/structidSearch.do?structureId=3GWH 3GWH] (PRD-II) {{PubMed|19684596}}
** [http://www.rcsb.org/pdb/explore.do?structureId=3rio 3RIO] (RBD-PRD-I) {{PubMed|22750856}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/O31691 O31691]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU13880]

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glcT_1456092_1456958_1 glcT] {{PubMed|22383849}}

* '''Sigma factor:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' available in [[Stülke]] lab:
** GP109 (in frame deletion)
** GP778 (replacement of ''[[glcT]]'' and the ''[[ptsG]]-[[ptsH]]-[[ptsI]]'' operon by a spc cassette)
** GP926 (substitution of ''[[glcT]]'' and ''[[ptsG]]'' by a tet cassette)

* '''Expression vector:'''
** pGP124 (full length, in [[pWH844]]), available in [[Stülke]] lab
** pGP114 (amino acids 1-60, RNA-binding domain, in [[pWH844]]), available in [[Stülke]] lab
** pGP230 (amino acids 1-60, RNA-binding domain with thrombin cleavage site, in [[pWH844]]), available in [[Stülke]] lab
** pGP164 (both PRDs, in [[pWH844]]), in addition diverse expression vectors for phosphorylation site mutants and for RBD mutants (all in [[pWH844]]), available in [[Stülke]] lab
** pGP424 (PRDI, in [[pWH844]]), available in [[Stülke]] lab
** pGP425 (PRDII, in [[pWH844]]), available in [[Stülke]] lab
** pGP442 (PRDI, in [[pGP570]], with thrombin cleavage site), available in [[Stülke]] lab
** pGP443 (PRDII, in [[pGP570]], with thrombin cleavage site), available in [[Stülke]] lab
** pGP575 (amino acids 1-60, RNA-binding domain with Strep-tag, in [[pGP574]]), available in [[Stülke]] lab

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''FLAG-tag construct:''' GP1220 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab

* '''Antibody:'''

=Labs working on this gene/protein=

[[Stülke|Jörg Stülke]], University of Göttingen, Germany
[http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

=Your additional remarks=

=References=
==Reviews==
<pubmed> 9663674 18086213 </pubmed>
==Original publications==
'''Additional publications:''' {{PubMed|22750856}}
<pubmed>11902727 9765562 12437213 10543968 17074746 15155854 14527945 , 19684596 22722928 20939030 </pubmed>
[[Category:Protein-coding genes]]

Eno

2012-09-28T13:16:13Z

Frothe1:

* '''Description:''' enolase, glycolytic/ gluconeogenic enzyme, [[universally conserved protein]] 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''eno''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || enolase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || enzyme in glycolysis/ gluconeogenesis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU33900 eno]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/Eno Eno]
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 46,4 kDa, 4.49
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1290 bp, 430 amino acids
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yvbK]]'', ''[[pgm]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15395]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:eno_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=eno_3476555_3477847_-1 Expression at a glance]'''   {{PubMed|22383849}} [[Image:eno_expression.png|500px]]
|-
|}

__TOC__
 
 
 
 
 

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[carbon core metabolism]]}},
{{SubtiWiki category|[[membrane proteins]]}},
{{SubtiWiki category|[[phosphoproteins]]}},
{{SubtiWiki category|[[universally conserved proteins]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[CggR regulon]]}}

=The gene=

=== Basic information ===
* '''Locus tag:''' BSU33900

===Phenotypes of a mutant ===
* no growth on LB, requires glucose and malate
* essential according to Kobayashi et al. on LB [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cggR-gapA-pgk-tpiA-pgm-eno.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10899]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' 2-phospho-D-glycerate = phosphoenolpyruvate + H2O (according to Swiss-Prot) 2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O

* '''Protein family:''' enolase family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:''' reversible Michaelis-Menten [http://www.ncbi.nlm.nih.gov/sites/entrez/25885 PubMed]

* '''Domains:'''
** substrate binding domain (366–369)

* '''Modification:''' phosphorylation on Thr-141 AND Ser-259 AND Tyr-281 AND Ser-325 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/16493705 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed]

* '''Cofactor(s):''' Mg2+

* '''Effectors of protein activity:'''
** Inhibited by EDTA [http://www.ncbi.nlm.nih.gov/sites/entrez/25885 PubMed]

* '''[[SubtInteract|Interactions]]:'''
** forms octamers {{PubMed|22198292}}
** part of the [[RNA degradosome]] {{PubMed|19193632}}
** [[Eno]]-[[PfkA]] {{PubMed|19193632}}, K(D) of the interaction: 40 nM {{PubMed|22198292}}
** [[Eno]]-[[Rny]] {{PubMed|19193632,21803996}}, K(D) of the interaction: 100 nM {{PubMed|22198292}}
** [[Eno]]-[[CshA]] {{PubMed|20572937}}

* '''[[Localization]]:'''
** cytoplasm [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
** membrane associated [http://www.ncbi.nlm.nih.gov/sites/entrez/18763711 PubMed]
** exported, this requires a long, unbent α-helix (from A108 to L126) {{PubMed|15003462,21856851}}

=== Database entries ===

* '''Structure:'''
** [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=4A3R 4A3R] {{PubMed|22198292}}
** [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1W6T 1W6T] (from ''Streptococcus pneumoniae'') {{PubMed|15476816}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/P37869 P37869]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU33900]

* '''E.C. number:''' [http://www.expasy.org/enzyme/4.2.1.11 4.2.1.11]

=== Additional information===
* Enolase is a [[moonlighting proteins|moonlighting protein]]. [http://www.ncbi.nlm.nih.gov/sites/entrez/19193632 PubMed]
* There are indications that this enzyme is an octamer [http://www.ncbi.nlm.nih.gov/sites/entrez/25885 PubMed]
* [[universally conserved protein]]
* extensive information on the structure and enzymatic properties of Eno can be found at [http://www.proteopedia.org/wiki/index.php/Enolase Proteopedia]

=Expression and regulation=

* '''Operon:'''
** ''[[cggR]]-[[gapA]]-[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]'' {{PubMed|11489127}}
** ''[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]'' {{PubMed|11489127}}

* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=eno_3476555_3477847_-1 eno] {{PubMed|22383849}}

* '''Sigma factor:''' [[SigA]] {{PubMed|11489127}}

* '''Regulation:'''
** expression activated by glucose (3.3 fold) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
** ''[[cggR]]'': induced by glycolytic substrates [[CggR]] {{PubMed|11489127}}
** ''[[pgk]]'': constitutive {{PubMed|11489127}}

* '''Regulatory mechanism:''' transcription repression by [[CggR]] {{PubMed|11489127}}

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''
** GP594 (''eno''::''cat''), available in [[Stülke]] lab
** GP599 (''eno''::''erm''), available in [[Stülke]] lab
** GP698 (''eno''-''[[pgm]]''::''cat''), available in [[Stülke]] lab
** GP1215 (''eno''-''Strep'' ''(spc)''), purification from ''B. subtilis'', for [[SPINE]], available in [[Stülke]] lab

* '''Expression vector:'''
** pGP1426 (expression of ''[[eno]]'' in ''B. subtilis'', in [[pBQ200]]), available in [[Stülke]] lab
** pGP1500 (expression of ''[[pgm]]'' and ''[[eno]]'' in ''B. subtilis'', in [[pBQ200]]), available in [[Stülke]] lab
** pGP563 (N-terminal His-tag, in [[pWH844]]), available in [[Stülke]] lab
** pGP1276 (N-terminal Strep-tag, purification from ''E. coli'', in [[pGP172]]), available in [[Stülke]] lab
** pGP93 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab

* '''lacZ fusion:'''
** see ''[[pgk]]''

* '''GFP fusion:''' pHT315-yfp-eno, available in [[Mijakovic]] lab

* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab

* '''FLAG-tag construct:''' GP1214 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab

* '''Antibody:''' available in [[Stülke]] lab

=Labs working on this gene/protein=

[[Stülke|Jörg Stülke]], University of Göttingen, Germany
[http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

=Your additional remarks=

=References=
==Reviews==
<pubmed> 8994873 </pubmed>
==Subcellular localization of enolase==
'''Additional publications:''' {{PubMed|21856851}}
<pubmed> 16479537 18763711 15003462 20497499 </pubmed>

==Other original publications==
<pubmed> 17726680, 17218307, 12850135, 19193632, 11489127, 8021172, 17505547, 25885, 20572937 15476816 9988532 ,21803996 22198292 </pubmed>

[[Category:Protein-coding genes]]

YoaA

2011-07-26T08:25:17Z

Frothe1:

* '''Description:''' similar to ribosomal-protein-alanine N-acetyltransferase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yoaA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 18 kDa, 5.92
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 495 bp, 165 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yoxB]]'', ''[[yoaB]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13746]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:yoaA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[translation]]}},
{{SubtiWiki category|[[protein modification]]}},
{{SubtiWiki category|[[general stress proteins (controlled by SigB)]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[SigB regulon]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU18530

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yoxCB-yoaA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13472]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:'''

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/O34569 O34569]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU18530]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[yoxC]]-[[yoxB]]-[[yoaA]]'' {{PubMed|10482513}}

* '''[[Sigma factor]]:''' [[SigB]] {{PubMed|10482513,15805528}}

* '''Regulation:'''
** induced by stress ([[SigB]]) {{PubMed|10482513,15805528}}

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''
** GP1248 (''yoaA''::''tet''), available in [[Stülke]] lab
** GP1255 (''acuA''::''kan'' ''yjck''::''cat'' ''ynaD''::''spec'' ''yoaA''::''tet''), available in [[Stülke]] lab

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=
<pubmed> 10482513,15805528</pubmed>
[[Category:Protein-coding genes]]

YnaD

2011-07-26T08:24:02Z

Frothe1:

* '''Description:''' similar to ribosomal-protein-alanine N-acetyltransferase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ynaD''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 20 kDa, 5.342
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 510 bp, 170 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ynaC]]'', ''[[ynaE]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13636]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:ynaD_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[translation]]}},
{{SubtiWiki category|[[protein modification]]}}

= This gene is a member of the following [[regulons]] =

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU17520

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12256]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:'''

* '''Protein family:''' acetyltransferase family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P94482 P94482]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU17520]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''
** GP1247 (''ynaD''::''spec''), available in [[Stülke]] lab
** GP1255 (''acuA''::''kan'' ''yjck''::''cat'' ''ynaD''::''spec'' ''yoaA''::''tet''), available in [[Stülke]] lab

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

[[Category:Protein-coding genes]]

YjcK

2011-07-26T08:22:29Z

Frothe1:

* '''Description:''' similar to ribosomal-protein-alanine N-acetyltransferase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yjcK''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 20 kDa, 5.459
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 543 bp, 181 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[metC]]'', ''[[yjcL]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13046]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:yjcK_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[translation]]}},
{{SubtiWiki category|[[protein modification]]}}

= This gene is a member of the following [[regulons]] =

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU11890

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13164]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' Acetyl-CoA + ribosomal-protein L-alanine = CoA + ribosomal-protein N-acetyl-L-alanine (according to Swiss-Prot)

* '''Protein family:''' N-acetyltransferase domain (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:''' cytoplasm (according to Swiss-Prot), Cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/O31633 O31633]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU11890]

* '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.1.128 2.3.1.128]

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''
** GP1246 (''yjck''::''cat''), available in [[Stülke]] lab
** GP1255 (''acuA''::''kan'' ''yjck''::''cat'' ''ynaD''::''spec'' ''yoaA''::''tet''), available in [[Stülke]] lab

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed> 16479537 </pubmed>

[[Category:Protein-coding genes]]

YjcK

2011-07-26T08:21:26Z

Frothe1:

* '''Description:''' similar to ribosomal-protein-alanine N-acetyltransferase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yjcK''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 20 kDa, 5.459
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 543 bp, 181 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[metC]]'', ''[[yjcL]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13046]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:yjcK_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[translation]]}},
{{SubtiWiki category|[[protein modification]]}}

= This gene is a member of the following [[regulons]] =

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU11890

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13164]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' Acetyl-CoA + ribosomal-protein L-alanine = CoA + ribosomal-protein N-acetyl-L-alanine (according to Swiss-Prot)

* '''Protein family:''' N-acetyltransferase domain (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:''' cytoplasm (according to Swiss-Prot), Cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/O31633 O31633]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU11890]

* '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.1.128 2.3.1.128]

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''
** GP1246 (''yjck''::''cat''), available in [[Stülke]] lab
** GP1255 (''acuA''::''kan''''yjck''::''cat''''ynaD''::''spec''''yoaA''::''tet''), available in [[Stülke]] lab

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed> 16479537 </pubmed>

[[Category:Protein-coding genes]]

YjcK

2011-07-26T08:20:33Z

Frothe1:

* '''Description:''' similar to ribosomal-protein-alanine N-acetyltransferase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yjcK''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 20 kDa, 5.459
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 543 bp, 181 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[metC]]'', ''[[yjcL]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13046]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:yjcK_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[translation]]}},
{{SubtiWiki category|[[protein modification]]}}

= This gene is a member of the following [[regulons]] =

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU11890

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13164]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' Acetyl-CoA + ribosomal-protein L-alanine = CoA + ribosomal-protein N-acetyl-L-alanine (according to Swiss-Prot)

* '''Protein family:''' N-acetyltransferase domain (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:''' cytoplasm (according to Swiss-Prot), Cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/O31633 O31633]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU11890]

* '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.1.128 2.3.1.128]

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''
** GP1246 (''yjck''::''cat''), available in [[Stülke]] lab
** GP1255 (''acuA''::''kan'')(''yjck''::''cat'')(''ynaD''::''spec'')(''yoaA''::''tet''), available in [[Stülke]] lab

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed> 16479537 </pubmed>

[[Category:Protein-coding genes]]

YjcK

2011-07-26T08:16:34Z

Frothe1:

* '''Description:''' similar to ribosomal-protein-alanine N-acetyltransferase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yjcK''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 20 kDa, 5.459
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 543 bp, 181 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[metC]]'', ''[[yjcL]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13046]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:yjcK_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[translation]]}},
{{SubtiWiki category|[[protein modification]]}}

= This gene is a member of the following [[regulons]] =

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU11890

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13164]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' Acetyl-CoA + ribosomal-protein L-alanine = CoA + ribosomal-protein N-acetyl-L-alanine (according to Swiss-Prot)

* '''Protein family:''' N-acetyltransferase domain (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:''' cytoplasm (according to Swiss-Prot), Cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/O31633 O31633]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU11890]

* '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.1.128 2.3.1.128]

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''
** GP1211 (''yjck''::''cat''), available in [[Stülke]] lab

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed> 16479537 </pubmed>

[[Category:Protein-coding genes]]

PfkA

2011-02-14T09:36:54Z

Frothe1:

* '''Description:''' phosphofructokinase, glycolytic enzyme 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''pfkA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''pfk''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || 6-phosphofructokinase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || catabolic enzyme in glycolysis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism], [http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34,1 kDa, 6.14
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 957 bp, 319 amino acids
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pyk]]'', ''[[accA]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14879]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:pfkA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[carbon core metabolism]]}}

= This gene is a member of the following [[regulons]] =

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU29190

===Phenotypes of a mutant ===

* essential according to [http://www.ncbi.nlm.nih.gov/pubmed/12682299 Kobayashi'' et al.''], dispensable according to [http://www.ncbi.nlm.nih.gov/pubmed/20473954 Munoz-Marquez and Ponce-Rivas]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pfkA-pyk-ytzA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12644]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate (according to Swiss-Prot)

* '''Protein family:''' phosphofructokinase family (according to Swiss-Prot) phosphofructokinase family

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:''' Allosteric Regulation (Reversible) [http://www.ncbi.nlm.nih.gov/pubmed/4269800 PubMed]

* '''Domains:'''
** 3 x nucleotide binding domain (ATP) (21–25), (154–158), (171–187)

* '''Modification:'''

* '''Cofactor(s):''' Mg2+

* '''Effectors of protein activity:'''
** Inhibited by citrate, PEP (Hill Coefficient 3) and Ca2+ (competes with Mg2+) in ''B. licheniformes'' [http://www.ncbi.nlm.nih.gov/pubmed/4269800 PubMed].
** Inhibited by ATP (competitively) and f6p (non-competitively) in ''G. stearothermophillus'' [http://www.ncbi.nlm.nih.gov/pubmed/8136379 PubMed]
** Activated by GDP and ADP in lower concentrations (1mM); above that inhibition, competing with the ATP for the binding site (in ''G. stearothermophillus'') [http://www.ncbi.nlm.nih.gov/pubmed/7873536 PubMed]
** Activated by NH4+ [http://www.ncbi.nlm.nih.gov/pubmed/7873536 PubMed]

* '''Interactions:''' [[PfkA]]-[[Pgm]] {{PubMed|19193632}}, [[PfkA]]-[[Eno]] {{PubMed|19193632}}, [[PfkA]]-[[Rny]] {{PubMed|19193632}}, [[PfkA]]-[[PnpA]], [[PfkA]]-[[RnjA]] {{PubMed|19193632}}, [[Eno]]-[[CshA]] {{PubMed|20572937}}

* '''Localization:''' cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1MTO 1MTO] (mutant, complex with fructose-6-phosphate, Geobacillus stearothermophilus), [http://www.rcsb.org/pdb/explore.do?structureId=4PFK 4PFK] (Geobacillus stearothermophilus), ''Geobacillus stearothermophilus'' [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=58449 NCBI], Mutant form, complex with fructose-6-phosphate ''Geobacillus stearothermophilus'' [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=21480 NCBI]

* '''UniProt:''' [http://www.uniprot.org/uniprot/O34529 O34529]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29190]

* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.1.11 2.7.1.11]

=== Additional information===
PfkA is a [[moonlighting proteins|moonlighting protein]]. [http://www.ncbi.nlm.nih.gov/sites/entrez/19193632 PubMed]

=Expression and regulation=
* '''Operon:''' ''[[pfkA]]-[[pyk]]-[[ytzA]]'' {{PubMed|11489127}}

* '''Sigma factor:'''

* '''Regulation:'''
** twofold induced by glucose {{PubMed|11489127}}

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''
* GP590 (''pfkA''::''cat''), available in [[Stülke]] lab
* GP595 (''pfkA''::''erm''), available in [[Stülke]] lab

* '''Expression vector:'''
** for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], in [[pGP380]]: pGP87, available in [[Stülke]] lab
** for expression/ purification from ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP393, available in [[Stülke]] lab
** for expression in ''B. subtilis'', in [[pBQ200]]: pGP1422, available in [[Stülke]] lab
** for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [[SPINE]], in [[pGP382]]: pGP1266, available in [[Stülke]] lab

* '''lacZ fusion:''' pGP511 (in [[pAC6]]), available in [[Stülke]] lab

* '''GFP fusion:'''

* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab

* '''FLAG-tag construct:''' GP1019 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab

* '''Antibody:'''

=Labs working on this gene/protein=

[[Stülke|Jörg Stülke]], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

=Your additional remarks=

=References=

<pubmed> 16479537,19193632, 11489127, 8136379, 7873536 4269800 20473954 20572937</pubmed>

[[Category:Protein-coding genes]]

Eno

2011-02-14T09:27:32Z

Frothe1:

* '''Description:''' enolase, glycolytic/ gluconeogenic enzyme, [[universally conserved protein]] 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''eno''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || enolase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || enzyme in glycolysis/ gluconeogenesis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 46,4 kDa, 4.49
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1290 bp, 430 amino acids
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yvbK]]'', ''[[pgm]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15395]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:eno_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[carbon core metabolism]]}},
{{SubtiWiki category|[[membrane proteins]]}},
{{SubtiWiki category|[[phosphoproteins]]}},
{{SubtiWiki category|[[universally conserved proteins]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[CggR regulon]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU33900

===Phenotypes of a mutant ===
* no growth on LB, requires glucose and malate
* essential according to Kobayashi et al. on LB [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cggR-gapA-pgk-tpiA-pgm-eno.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10899]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' 2-phospho-D-glycerate = phosphoenolpyruvate + H2O (according to Swiss-Prot) 2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O

* '''Protein family:''' enolase family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:''' reversible Michaelis-Menten [http://www.ncbi.nlm.nih.gov/sites/entrez/25885 PubMed]

* '''Domains:'''
** substrate binding domain (366–369)

* '''Modification:''' phosphorylation on Thr-141 AND Ser-259 AND Tyr-281 AND Ser-325 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/16493705 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed]

* '''Cofactor(s):''' Mg2+

* '''Effectors of protein activity:'''
** Inhibited by EDTA [http://www.ncbi.nlm.nih.gov/sites/entrez/25885 PubMed]

* '''Interactions:''' [[Eno]]-[[PfkA]] {{PubMed|19193632}}, [[Eno]]-[[Rny]] {{PubMed|19193632}}, [[Eno]]-[[CshA]] {{PubMed|20572937}}

* '''Localization:''' cytoplasm [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed] and membrane associated [http://www.ncbi.nlm.nih.gov/sites/entrez/18763711 PubMed], exported {{PubMed|15003462}}

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1W6T 1W6T] (from ''Streptococcus pneumoniae'') {{PubMed|15476816}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/P37869 P37869]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU33900]

* '''E.C. number:''' [http://www.expasy.org/enzyme/4.2.1.11 4.2.1.11]

=== Additional information===
* Enolase is a [[moonlighting proteins|moonlighting protein]]. [http://www.ncbi.nlm.nih.gov/sites/entrez/19193632 PubMed]
* There are indications that this enzyme is an octamer [http://www.ncbi.nlm.nih.gov/sites/entrez/25885 PubMed]
* [[universally conserved protein]]

=Expression and regulation=

* '''Operon:'''
** ''[[cggR]]-[[gapA]]-[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]'' {{PubMed|11489127}}
** ''[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]'' {{PubMed|11489127}}

* '''Sigma factor:''' [[SigA]] {{PubMed|11489127}}

* '''Regulation:'''
** expression activated by glucose (3.3 fold) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
** ''[[cggR]]'': induced by glycolytic substrates [[CggR]] {{PubMed|11489127}}
** ''[[pgk]]'': constitutive {{PubMed|11489127}}

* '''Regulatory mechanism:''' transcription repression by [[CggR]] {{PubMed|11489127}}

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''
** GP594 (''eno''::''cat''), available in [[Stülke]] lab
** GP599 (''eno''::''erm''), available in [[Stülke]] lab
** GP698 (''eno''-''[[pgm]]''::''cat''), available in [[Stülke]] lab
** GP1214 (''eno''-''3xFlag'' ''(spc)''), available in [[Stülke]] lab
** GP1215 (''eno''-''Strep'' ''(spc)''), available in [[Stülke]] lab

* '''Expression vector:'''
** pGP1426 (expression of ''eno'' in ''B. subtilis'', in [[pBQ200]]), available in [[Stülke]] lab
** pGP399 (expression of ''eno'' from ''E. coli'' in ''B. subtilis'', in [[pBQ200]]), available in [[Stülke]] lab
** pGP563 (N-terminal His-tag, in [[pWH844]]), available in [[Stülke]] lab
** pGP93 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab
** pGP1500 (expression of ''[[pgm]]'' and ''[[eno]]'' in ''B. subtilis'', in [[pBQ200]]), available in [[Stülke]] lab
** pGP1276 (N-terminal Strep-tag, purification from ''E. coli'', in [[pGP172]]), available in [[Stülke]] lab

* '''lacZ fusion:'''
** see ''[[pgk]]''

* '''GFP fusion:''' pHT315-yfp-eno, available in [[Mijakovic]] lab

* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab

* '''Antibody:''' available in [[Stülke]] lab

=Labs working on this gene/protein=

[[Stülke|Jörg Stülke]], University of Göttingen, Germany
[http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

=Your additional remarks=

=References=
==Reviews==
<pubmed> 8994873 </pubmed>
==Subcellular localization of enolase==
<pubmed> 16479537 18763711 15003462 20497499 </pubmed>
==Other original publications==
<pubmed> 17726680, 17218307, 12850135, 19193632, 11489127, 8021172, 17505547, 25885, 20572937 15476816 9988532 </pubmed>

[[Category:Protein-coding genes]]

Eno

2011-02-14T09:19:52Z

Frothe1:

* '''Description:''' enolase, glycolytic/ gluconeogenic enzyme, [[universally conserved protein]] 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''eno''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || enolase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || enzyme in glycolysis/ gluconeogenesis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 46,4 kDa, 4.49
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1290 bp, 430 amino acids
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yvbK]]'', ''[[pgm]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15395]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:eno_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[carbon core metabolism]]}},
{{SubtiWiki category|[[membrane proteins]]}},
{{SubtiWiki category|[[phosphoproteins]]}},
{{SubtiWiki category|[[universally conserved proteins]]}}

= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[CggR regulon]]}}

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU33900

===Phenotypes of a mutant ===
* no growth on LB, requires glucose and malate
* essential according to Kobayashi et al. on LB [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cggR-gapA-pgk-tpiA-pgm-eno.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10899]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' 2-phospho-D-glycerate = phosphoenolpyruvate + H2O (according to Swiss-Prot) 2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O

* '''Protein family:''' enolase family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:''' reversible Michaelis-Menten [http://www.ncbi.nlm.nih.gov/sites/entrez/25885 PubMed]

* '''Domains:'''
** substrate binding domain (366–369)

* '''Modification:''' phosphorylation on Thr-141 AND Ser-259 AND Tyr-281 AND Ser-325 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/16493705 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed]

* '''Cofactor(s):''' Mg2+

* '''Effectors of protein activity:'''
** Inhibited by EDTA [http://www.ncbi.nlm.nih.gov/sites/entrez/25885 PubMed]

* '''Interactions:''' [[Eno]]-[[PfkA]] {{PubMed|19193632}}, [[Eno]]-[[Rny]] {{PubMed|19193632}}, [[Eno]]-[[CshA]] {{PubMed|20572937}}

* '''Localization:''' cytoplasm [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed] and membrane associated [http://www.ncbi.nlm.nih.gov/sites/entrez/18763711 PubMed], exported {{PubMed|15003462}}

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1W6T 1W6T] (from ''Streptococcus pneumoniae'') {{PubMed|15476816}}

* '''UniProt:''' [http://www.uniprot.org/uniprot/P37869 P37869]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU33900]

* '''E.C. number:''' [http://www.expasy.org/enzyme/4.2.1.11 4.2.1.11]

=== Additional information===
* Enolase is a [[moonlighting proteins|moonlighting protein]]. [http://www.ncbi.nlm.nih.gov/sites/entrez/19193632 PubMed]
* There are indications that this enzyme is an octamer [http://www.ncbi.nlm.nih.gov/sites/entrez/25885 PubMed]
* [[universally conserved protein]]

=Expression and regulation=

* '''Operon:'''
** ''[[cggR]]-[[gapA]]-[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]'' {{PubMed|11489127}}
** ''[[pgk]]-[[tpiA]]-[[pgm]]-[[eno]]'' {{PubMed|11489127}}

* '''Sigma factor:''' [[SigA]] {{PubMed|11489127}}

* '''Regulation:'''
** expression activated by glucose (3.3 fold) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
** ''[[cggR]]'': induced by glycolytic substrates [[CggR]] {{PubMed|11489127}}
** ''[[pgk]]'': constitutive {{PubMed|11489127}}

* '''Regulatory mechanism:''' transcription repression by [[CggR]] {{PubMed|11489127}}

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''
** GP594 (''eno''::''cat''), available in [[Stülke]] lab
** GP599 (''eno''::''erm''), available in [[Stülke]] lab
** GP698 (''eno''-''[[pgm]]''::''cat''), available in [[Stülke]] lab
** GP1214 (''eno''-''3xFlag'' ''(spc)''), available in [[Stülke]] lab
** GP1215 (''eno''-''Strep'' ''(spc)''), available in [[Stülke]] lab

* '''Expression vector:'''
** pGP1426 (expression of ''eno'' in ''B. subtilis'', in [[pBQ200]]), available in [[Stülke]] lab
** pGP399 (expression of ''eno'' from ''E. coli'' in ''B. subtilis'', in [[pBQ200]]), available in [[Stülke]] lab
** pGP563 (N-terminal His-tag, in [[pWH844]]), available in [[Stülke]] lab
** pGP93 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab
** pGP1500 (expression of ''[[pgm]]'' and ''[[eno]]'' in ''B. subtilis'', in [[pBQ200]]), available in [[Stülke]] lab

* '''lacZ fusion:'''
** see ''[[pgk]]''

* '''GFP fusion:''' pHT315-yfp-eno, available in [[Mijakovic]] lab

* '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab

* '''Antibody:''' available in [[Stülke]] lab

=Labs working on this gene/protein=

[[Stülke|Jörg Stülke]], University of Göttingen, Germany
[http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

=Your additional remarks=

=References=
==Reviews==
<pubmed> 8994873 </pubmed>
==Subcellular localization of enolase==
<pubmed> 16479537 18763711 15003462 20497499 </pubmed>
==Other original publications==
<pubmed> 17726680, 17218307, 12850135, 19193632, 11489127, 8021172, 17505547, 25885, 20572937 15476816 9988532 </pubmed>

[[Category:Protein-coding genes]]

AcuC

2010-08-16T08:42:41Z

Frothe1:

* '''Description:''' protein deacetylase for the control of [[AcsA]] activity 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''acuC''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || [[AcsA]] deacetylase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || control of [[AcsA]] activity
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 42 kDa, 5.062
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1161 bp, 387 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[acuB]]'', ''[[motS]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14949]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:acuC_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU29710

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/acuABC.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10367]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' deacetylates (and thereby activates) [[AcsA]] (together with [[SrtN]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/19136592 PubMed]

* '''Protein family:''' histone deacetylase family (according to Swiss-Prot) class I histone deacetylase

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P39067 P39067]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29710]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=
* '''Operon:''' ''[[acuA]]-[[acuB]]-[[acuC]]'' {{PubMed|7934817}}

* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|7913927}}

* '''Regulation:'''
** repressed by glucose ([[CcpA]]) {{PubMed|7913927,12850135}}

* '''Regulatory mechanism:'''
** [[CcpA]]: transcription repression {{PubMed|7913927}}

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''
** GP1209 (''acuC''::''kan''), available in [[Stülke]] lab
** GP1213(''srtN''::''cat'')(''acuC''::''kan''), available in [[Stülke]] lab
* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>7913927,,16855235,7934817, 19136592, 18487328 </pubmed>

[[Category:Protein-coding genes]]

SrtN

2010-08-16T08:40:47Z

Frothe1:

* '''Description:''' protein deacetylase for the control of [[AcsA]] activity 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''srtN''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yhdZ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || NAD(+)-dependent [[AcsA]] deacetylase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || control of [[AcsA]] activity
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 5.134
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 741 bp, 247 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhdY]]'', ''[[yheN]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12804]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:yhdZ_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU09650

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13032]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein (according to Swiss-Prot) deacetylates (and thereby activates) [[AcsA]] (together with [[AcuC]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/19136592 PubMed]

* '''Protein family:''' deacetylase sirtuin-type domain (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:''' cytoplasm (according to Swiss-Prot)

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/O07595 O07595]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU09650]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''
** GP1210 (''srtN''::''cat''), available in [[Stülke]] lab
** GP1213(''srtN''::''cat'')(''acuC''::''kan''), available in [[Stülke]] lab
* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed> 19136592 </pubmed>

[[Category:Protein-coding genes]]

AcsA

2010-08-16T08:37:29Z

Frothe1:

* '''Description:''' acetyl-CoA synthetase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''acsA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || acetyl-CoA synthetase)
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of acetate, fatty acids
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 64 kDa, 5.547
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1716 bp, 572 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[tyrS]]'', ''[[acuA]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14946]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:acsA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU29680

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/acsA.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10370]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA (according to Swiss-Prot)

* '''Protein family:''' ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:''' acetylated on Lys-549 by [[AcuA]], this results in inactivation [http://www.ncbi.nlm.nih.gov/sites/entrez/18487328 PubMed], deacetylated by [[SrtN]] and [[AcuC]] deacetylates (and thereby activates) [[AcsA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19136592 PubMed]

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P39062 P39062]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29680]

* '''E.C. number:''' [http://www.expasy.org/enzyme/6.2.1.1 6.2.1.1]

=== Additional information===

=Expression and regulation=
* '''Operon:''' ''[[acsA]]'' {{PubMed|7934817}}

* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|7913927}}

* '''Regulation:'''
** repressed by glucose (4.5-fold) ([[CcpA]]) {{PubMed|7913927,12850135}}
** repressed during growth in the presence of branched chain amino acids ([[CodY]]) {{PubMed|12618455,18083814}}

* '''Regulatory mechanism:'''
** [[CcpA]]: transcription repression {{PubMed|7913927}}
** [[CodY]]: transcription repression {{PubMed|12618455,18083814}}

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''
** GP1212 (''acsA''::''kan''), available in [[Stülke]] lab
* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>16855235,7934817,7913927,18083814,17303131,12618455,12618455, 19136592, 18487328, 12850135 </pubmed>

[[Category:Protein-coding genes]]

SrtN

2010-08-16T08:35:53Z

Frothe1:

* '''Description:''' protein deacetylase for the control of [[AcsA]] activity 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''srtN''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yhdZ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || NAD(+)-dependent [[AcsA]] deacetylase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || control of [[AcsA]] activity
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 5.134
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 741 bp, 247 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhdY]]'', ''[[yheN]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12804]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:yhdZ_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU09650

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13032]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein (according to Swiss-Prot) deacetylates (and thereby activates) [[AcsA]] (together with [[AcuC]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/19136592 PubMed]

* '''Protein family:''' deacetylase sirtuin-type domain (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:''' cytoplasm (according to Swiss-Prot)

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/O07595 O07595]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU09650]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''
** GP1210 (''srtN''::''cat''), available in [[Stülke]] lab
* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed> 19136592 </pubmed>

[[Category:Protein-coding genes]]

AcuC

2010-08-16T08:33:01Z

Frothe1:

* '''Description:''' protein deacetylase for the control of [[AcsA]] activity 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''acuC''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || [[AcsA]] deacetylase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || control of [[AcsA]] activity
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 42 kDa, 5.062
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1161 bp, 387 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[acuB]]'', ''[[motS]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14949]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:acuC_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU29710

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/acuABC.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10367]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' deacetylates (and thereby activates) [[AcsA]] (together with [[SrtN]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/19136592 PubMed]

* '''Protein family:''' histone deacetylase family (according to Swiss-Prot) class I histone deacetylase

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P39067 P39067]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29710]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=
* '''Operon:''' ''[[acuA]]-[[acuB]]-[[acuC]]'' {{PubMed|7934817}}

* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|7913927}}

* '''Regulation:'''
** repressed by glucose ([[CcpA]]) {{PubMed|7913927,12850135}}

* '''Regulatory mechanism:'''
** [[CcpA]]: transcription repression {{PubMed|7913927}}

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''
** GP1209 (''acuC''::''kan''), available in [[Stülke]] lab
* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>7913927,,16855235,7934817, 19136592, 18487328 </pubmed>

[[Category:Protein-coding genes]]

AcuA

2010-08-16T08:31:27Z

Frothe1:

* '''Description:''' protein acetylase for the control of [[AcsA]] activity 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''acuA''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || Gcn5-related N-acetyltransferase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || control of [[AcsA]] activity
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 24 kDa, 5.028
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 630 bp, 210 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[acsA]]'', ''[[acuB]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14947]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:acuA_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU29690

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/acuABC.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10369]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' acetylates (and thereby inactivates) [[AcsA]] on Lys-549 [http://www.ncbi.nlm.nih.gov/sites/entrez/18487328 PubMed] [http://www.ncbi.nlm.nih.gov/sites/entrez/19136592 PubMed]

* '''Protein family:''' acetyltransferase family (according to Swiss-Prot) Gcn5-relatedN-acetyltransferase [http://www.ncbi.nlm.nih.gov/sites/entrez/18487328 PubMed]

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''UniProt:''' [http://www.uniprot.org/uniprot/P39065 P39065]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29690]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=
* '''Operon:''' ''[[acuA]]-[[acuB]]-[[acuC]]'' {{PubMed|7934817}}

* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|7913927}}

* '''Regulation:'''
** repressed by glucose (3.4-fold) ([[CcpA]]) {{PubMed|7913927,12850135}}

* '''Regulatory mechanism:'''
** [[CcpA]]: transcription repression {{PubMed|7913927}}

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''
** GP1211 (''acuA''::''kan''), available in [[Stülke]] lab
* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed>7913927,,16855235,7934817, 19136592, 18487328, 12850135 </pubmed>

[[Category:Protein-coding genes]]