SubtiWiki - User contributions [en]

SacY

2009-06-19T08:41:59Z

Dlecoq: /* References */

* '''Description:''' transcriptional antiterminator for ''[[sacB]]'' and ''[[sacX]]-[[sacY]]'' (acts at high sucrose concentrations) 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''sacY''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipa-13r, sacS ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional antiterminator
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of sucrose utilization
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism], [http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 5.883
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 840 bp, 280 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sacX]]'', ''[[gspA]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15868]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:sacY_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU38420

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sacXY.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10559]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' binding to the mRNA of'' [[sacB]]'' and the ''[[sacX]]-[[sacY]]'' operon, causes transcription antitermination (in presence of sucrose)

* '''Protein family:''' transcriptional antiterminator bglG family (according to Swiss-Prot) BglG family of antiterminators

* '''Paralogous protein(s):''' [[LicT]], [[GlcT]], [[SacT]]

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:''' [[PtsH]]-[[SacY]]

* '''Localization:'''

=== Database entries ===

* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1AUU 1AUU] (RNA-binding domain)

* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P15401 P15401]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU38420]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[sacX]]-[[sacY]]''

* '''[[Sigma factor]]:''' [[SigA]]

* '''Regulation:''' induction by sucrose (at high concentration)

* '''Regulatory mechanism:''' induction by [[SacY]]-dependent RNA switch (transcriptional antitermination)

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Stephane Aymerich |Stephane Aymerich]], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

=Your additional remarks=

=References=

<pubmed>2105292 8702561 11580842 1279678 9305643 9305644 9202047 10610766, </pubmed>

GlpX

2009-06-18T14:18:41Z

Dlecoq: /* Database entries */

* '''Description:''' class II fructose-1,6-bisphosphatase, gluconeogenesis 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''glpX''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywjI ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || class II fructose-1,6-bisphosphatase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || gluconeogenesis
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism], [http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 5.067
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 963 bp, 321 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rho]]'', ''[[murAB]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15726]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:ywjI_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU37090

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ywjI.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10415]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' D-fructose 1,6-bisphosphate ==> D-fructose 6-phosphate + Pi

* '''Protein family:''' FBPase class 2 family (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:''' K(M) for FBP: 20 µM [http://www.ncbi.nlm.nih.gov/sites/entrez/19270101 PubMed]

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/Q03224 Q03224]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU37090]

* '''E.C. number:''' 3.1.3.11

=== Additional information===

=Expression and regulation=

* '''Operon:''''' [[murAB]]-[[glpX]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/19270101 PubMed]

* '''[[Sigma factor]]:'''

* '''Regulation:''' constitutive [http://www.ncbi.nlm.nih.gov/sites/entrez/19270101 PubMed]

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

=Your additional remarks=

=References=

<pubmed> 19270101 </pubmed>

HisJ

2009-06-18T14:08:39Z

Dlecoq: /* Phenotypes of a mutant */

* '''Description:''' histidinol phosphate phosphatase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''hisJ''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ytvP ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || histidinol phosphate phosphatase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of histidine
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/histidine.html His]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 30 kDa, 5.061
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 804 bp, 268 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ezrA]]'', ''[[yttP]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14940]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:hisJ_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU29620

===Phenotypes of a mutant ===

auxotroph for histidine

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13931]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' L-histidinol phosphate + H2O = L-histidinol + phosphate (according to Swiss-Prot)

* '''Protein family:''' HisK subfamily (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O34411 O34411]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU29620]

* '''E.C. number:''' [http://www.expasy.org/enzyme/3.1.3.15 3.1.3.15]

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Stephane Aymerich |Stephane Aymerich]], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

=Your additional remarks=

=References=

''D Le Coq, S Fillinger, S Aymerich''

'''Histidinol phosphate phosphatase, catalyzing the penultimate step of the histidine biosynthesis pathway, is encoded by ytvP (hisJ) in Bacillus subtilis.'''

J. Bacteriol.: 1999, 181(10);3277-80

[http://www.ncbi.nlm.nih.gov/pubmed/10322033]

HisJ

2009-06-18T14:04:55Z

Dlecoq: /* References */

* '''Description:''' histidinol phosphate phosphatase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''hisJ''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ytvP ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || histidinol phosphate phosphatase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of histidine
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/histidine.html His]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 30 kDa, 5.061
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 804 bp, 268 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ezrA]]'', ''[[yttP]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14940]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:hisJ_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU29620

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13931]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' L-histidinol phosphate + H2O = L-histidinol + phosphate (according to Swiss-Prot)

* '''Protein family:''' HisK subfamily (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O34411 O34411]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU29620]

* '''E.C. number:''' [http://www.expasy.org/enzyme/3.1.3.15 3.1.3.15]

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Stephane Aymerich |Stephane Aymerich]], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

=Your additional remarks=

=References=

''D Le Coq, S Fillinger, S Aymerich''

'''Histidinol phosphate phosphatase, catalyzing the penultimate step of the histidine biosynthesis pathway, is encoded by ytvP (hisJ) in Bacillus subtilis.'''

J. Bacteriol.: 1999, 181(10);3277-80

[http://www.ncbi.nlm.nih.gov/pubmed/10322033]

HisJ

2009-06-18T13:58:58Z

Dlecoq: /* References */

* '''Description:''' histidinol phosphate phosphatase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''hisJ''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ytvP ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || histidinol phosphate phosphatase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of histidine
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/histidine.html His]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 30 kDa, 5.061
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 804 bp, 268 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ezrA]]'', ''[[yttP]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14940]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:hisJ_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU29620

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13931]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' L-histidinol phosphate + H2O = L-histidinol + phosphate (according to Swiss-Prot)

* '''Protein family:''' HisK subfamily (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O34411 O34411]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU29620]

* '''E.C. number:''' [http://www.expasy.org/enzyme/3.1.3.15 3.1.3.15]

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Stephane Aymerich |Stephane Aymerich]], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

=Your additional remarks=

=References=

''D Le Coq, S Fillinger, S Aymerich''

'''Histidinol phosphate phosphatase, catalyzing the penultimate step of the histidine biosynthesis pathway, is encoded by ytvP (hisJ) in Bacillus subtilis.'''

J. Bacteriol.: 1999, 181(10);3277-80

[http://pubmed/10322033] (P p)

HisJ

2009-06-18T13:46:51Z

Dlecoq: /* References */

* '''Description:''' histidinol phosphate phosphatase 

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''hisJ''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ytvP ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || histidinol phosphate phosphatase
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of histidine
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: [http://subtiwiki.uni-goettingen.de/pathways/histidine.html His]'''
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 30 kDa, 5.061
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 804 bp, 268 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ezrA]]'', ''[[yttP]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14940]+-newId sequences] (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' [[Image:hisJ_context.gif]]
<div align="right"> This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</div>
|-
|}

__TOC__

 

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU29620

===Phenotypes of a mutant ===

=== Database entries ===

* '''DBTBS entry:''' no entry

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13931]

=== Additional information===

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' L-histidinol phosphate + H2O = L-histidinol + phosphate (according to Swiss-Prot)

* '''Protein family:''' HisK subfamily (according to Swiss-Prot)

* '''Paralogous protein(s):'''

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:'''

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:'''

* '''Localization:'''

=== Database entries ===

* '''Structure:'''

* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O34411 O34411]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU29620]

* '''E.C. number:''' [http://www.expasy.org/enzyme/3.1.3.15 3.1.3.15]

=== Additional information===

=Expression and regulation=

* '''Operon:'''

* '''[[Sigma factor]]:'''

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:'''

* '''Expression vector:'''

* '''lacZ fusion:'''

* '''GFP fusion:'''

* '''two-hybrid system:'''

* '''Antibody:'''

=Labs working on this gene/protein=

[[Stephane Aymerich |Stephane Aymerich]], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

=Your additional remarks=

=References=
D Le Coq, S Fillinger, S Aymerich
Histidinol phosphate phosphatase, catalyzing the penultimate step of the histidine biosynthesis pathway, is encoded by ytvP (hisJ) in Bacillus subtilis.
J. Bacteriol.: 1999, 181(10);3277-80
[PubMed:10322033] (P p)