MreC

2010-06-25T20:38:50Z

CauseyLori: /* Biological materials */

* '''Description:''' MreC is a cell shape determining protein and is associated with the [[MreB]] cytoskeleton in ''B. subtilis'' and other rod shaped bacteria.<br/><br/>

{| align="right" border="1" cellpadding="2"
|-
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''mreC''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || cell-shape determining protein
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || cell-shape determation
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 6.248
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 870 bp, 290 aa
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mreD]]'', ''[[mreB]]''
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14762]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
|-
|-
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:mreC_context.gif]]
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
|-
|}

__TOC__

<br/><br/>

=The gene=

=== Basic information ===

* '''Locus tag:''' BSU28020

===Phenotypes of a mutant ===
''mreC'' is essential under normal conditions {{PubMed|12682299}}. Depletion of MreC leads to a progressive increase in the width and a decrease in the length of the cell. This shape defect is consistent with a role for ''mreC'' in cell wall synthesis during elongation and has a similar phenotype to other genes with roles in elongation like ''[[rodA]]'' and the redundant gene pair ''[[pbpA]]'' and ''[[pbpH]]''. Electron microscopy of cells depleted of MreC shows regions of the cell where a thick and irregular cell wall has accumulated {{PubMed|12867458,16101995}}. ''mreC'' can be deleted provided that 0.5 M sucrose and 20 mM Mg(2+) is provided in the media, ''mreC'' is therefore conditionally essentail. The phenotype of the ''mreC'' deletion in these conditions is one characterised by extreamly fat and bloated cells that tend to grow in clusters {{PubMed|16101995}}.

=== Database entries ===

* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mreBCD-minCD.html]

* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10327]

= Additional information=

===Function===
MreC functions in cell wall synthesis by, together with the [[MreB]] cytoskeleton, localizing the cell wall synthetic machinery to the correct part of the cell. MreC therefore ensures that the cell wall is made in the correct way to maintain the proper shape of the cell.

===MreC in other organisms===
MreC has been studied in other organisms where it has been shown to be important in cell shape determination.

*''Escherichia coli'' [http://www.ncbi.nlm.nih.gov/pubmed/15612918 PubMed] [http://www.ncbi.nlm.nih.gov/pubmed/17993535 PubMed]
*''Caulobacter cresentus'' [http://www.ncbi.nlm.nih.gov/pubmed/16344480 PubMed] [http://www.ncbi.nlm.nih.gov/pubmed/16344481 PubMed]
*''Rhodobacter spheroides'' [http://www.ncbi.nlm.nih.gov/pubmed/16484180 PubMed]
*''Streptomyces coelicolor'' [http://www.ncbi.nlm.nih.gov/pubmed/10954092 PubMed]

=The protein=

=== Basic information/ Evolution ===

* '''Catalyzed reaction/ biological activity:''' None/ structural protein

* '''Protein family:''' mreC family (according to Swiss-Prot) COG1793

* '''Paralogous protein(s):''' None

=== Extended information on the protein ===

* '''Kinetic information:'''

* '''Domains:''' Intracellular N-terminus, transmembrane domain, Coiled coil domain and C-terminal beta-sheet domain.

* '''Modification:'''

* '''Cofactor(s):'''

* '''Effectors of protein activity:'''

* '''Interactions:''' [[MreC]]-[[MreD]], interacts also with a subset of the [[penicillin-binding protein|PBPs]] [http://www.ncbi.nlm.nih.gov/pubmed/17427287 PubMed]

* '''Localization:''' cell membrane (according to Swiss-Prot), GFP-MreC localises to the cell membrane in a helical pattern [http://www.ncbi.nlm.nih.gov/pubmed/16101995 PubMed].

=== Database entries ===

* '''Structure:'''
** [http://www.rcsb.org/pdb/explore.do?structureId=2J5U 2J5U]: MreC from ''Listeria monocytogenes'' [http://www.ncbi.nlm.nih.gov/pubmed/17427287 PubMed]
** [http://www.rcsb.org/pdb/explore.do?structureId=2QF4 2QF4]: MreC monomer from ''Streptococcus pneumoniae'' [http://www.ncbi.nlm.nih.gov/pubmed/17707860 PubMed]
** [http://www.rcsb.org/pdb/explore.do?structureId=2QF5 2QF5]: MreC dimer from ''Streptococcus pneumoniae'' [http://www.ncbi.nlm.nih.gov/pubmed/17707860 PubMed]

* '''UniProt:''' [http://www.uniprot.org/uniprot/Q01466 Q01466]

* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU28020]

* '''E.C. number:'''

=== Additional information===

=Expression and regulation=

* '''Operon:''' ''[[mreB]]-[[mreC]]-[[mreD]]-[[minC]]-[[minD]]'' {{PubMed|8459776}}

* '''[[Sigma factor]]:''' [[SigH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/8459776 PubMed]

* '''Regulation:'''

* '''Regulatory mechanism:'''

* '''Additional information:'''

=Biological materials =

* '''Mutant:''' A non-polar inframe deletion strain named 3481 and a xylose dependent conditional mutant named 3461 is avaliable from the [[Errington]] lab [http://www.ncbi.nlm.nih.gov/pubmed/16101995 PubMed].

* '''Expression vector:''' [http://www.poker-rooms-review.org/uk-online-poker/everest-poker-review/ everest poker]

* '''lacZ fusion:'''

* '''GFP fusion:''' A functional N-terminal GFP fusion has been made where the fusion protein is the only copy of the gene in the cell: strain 3417 [http://www.ncbi.nlm.nih.gov/pubmed/16101995 PubMed].

* '''two-hybrid system:'''

* '''Antibody:''' antisera raised in rabit is avaliable from the [[Errington]] lab.

=Labs working on this gene/protein=

[[Jeff Errington]], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]

[[Peter Graumann]], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]

=Your additional remarks=

''mreC'' is an abbreviation of murein region e, gene C

=References=

<pubmed>18784364,16101995,1400224,18156271,17427287,,12867458 16101995 15612918 17993535 16344480 17427287 16344481 16484180 10954092 19643765 8459776</pubmed>

[[Category:Protein-coding genes]]

SubtiWiki - User contributions [en]

MreC