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ccpC [Tue Dec 13 2016 11:16:32 GMT+0100 (CET)]
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ccpC [Tue Dec 13 2016 11:16:32 GMT+0100 (CET)]

transcriptional repressor of citZ and citB
locus
BSU14140
pI
5.93
mw
33.00 kDa
function
regulation of tricarboxylic acid branch of the TCA cycle
product
transcriptional repressor (LysR family)
essential
no
synonyms
ykuM
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.4|TCA cycle]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.5|Regulators of core metabolism]

    • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism], [SW|transcription factors and their control], [SW|regulators of core metabolism]

    • This gene is a member of the following [SW|regulons]

  • [SW|CcpA regulon], [SW|CcpC regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    1,486,045 -> 1,486,926

    The protein

    Protein family

  • [SW|LysR family]

    • Expression and Regulation

    Operon

  • ''[protein|search|ykuJ]-[protein|search|ykuK]-[protein|search|abbA]-[protein|search|ykuL]-[protein|search|ccpC]'' [Pubmed|16395550]
  • ''[protein|search|ccpC]'' [Pubmed|16395550]

    • [SW|Sigma factor]

  • ''[protein|search|ccpC]'': [protein|search|SigA]

    • Regulation

  • repressed in the presence of glucose and glutamate ([protein|search|CcpC]) [Pubmed|11985717]

    • Regulatory mechanism

  • [protein|search|CcpC]: transcription repression (molecular inducer: citrate) [Pubmed|11985717]
  • [protein|search|CcpA]: transcription repression [Pubmed|16395550]

    • Biological materials

    Mutant

  • GP206 (''aphA3'') & GP1442 (''aphA3'') , available in [SW|Stülke] lab

    • Expression vector

  • for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP706, available in [SW|Stülke] lab

    • lacZ fusion

  • pGP707 (in [protein|search|pAC5]), available in [SW|Stülke] lab, pGP711 (in [protein|search|pAC6]), available in [SW|Stülke] lab

    • Labs working on this gene/protein

  • [SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]

    • References

  • 17994626,23139400,11985717,10656796,12591885,14636591,16395550