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exoR [2019-07-10 17:42:13]
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exoR [2019-07-10 17:42:13]

5'->3' exonuclease, contributes to the removal of RNA from an Okazaki fragment, protection of developing and dormant spores against UV DNA damage
locus
BSU22010
pI
5.22
mw
32.79 kDa
protein length
296 aa Sequence Blast
gene length
888 bp Sequence Blast
function
removal of RNA from Okazaki fragments
product
5'->3' exonuclease
essential
no
synonyms
ypcP,exoA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]

    • Gene

    Coordinates
    2,310,995 → 2,311,885

    Phenotypes of a mutant

  • a [gene|3D6DA8B52A4CB49361E67906140696FFA1EF6099|polA] [gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|exoR] double mutant is not viable (synthetic lethality) [pubmed|17114254]
  • a [gene|B8B0F6B129475FDEEA8D538A51C153BF9A35887D|rnhC] [gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|exoR] double mutant is not viable at 25°C [pubmed|30670546]

    • The protein

    Catalyzed reaction/ biological activity

  • removal of RNA from Okazaki fragments [pubmed|30670546]

    • Paralogous protein(s)

  • [protein|3D6DA8B52A4CB49361E67906140696FFA1EF6099|PolA]:

    • Structure

  • [PDB|1BGX] (Taq polymerase, 36% identity) [pubmed|9770525]

    • [SW|Localization]

  • recruited to replication forks after induction of DNA damage [pubmed|31251806]

    • Expression and Regulation

    Operons

    genes
    [gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|exoR]
    description
    [pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-B868 (exoA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1867 NBRP B. subtilis, Japan]
  • BKE22010 (Δ[gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|exoR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAATAACTCCCTTTTC, downstream forward: _UP4_GCTAGAGAGATCGTTTAGAT
  • BKK22010 (Δ[gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|exoR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAATAACTCCCTTTTC, downstream forward: _UP4_GCTAGAGAGATCGTTTAGAT

    • References

  • 17114254,17905985,16045613,30096406,9770525,30670546,31251806