SubtiBank SubtiBank
acuB
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acuB

unknown
locus
BSU_29700
pI
6.59
mw
24.21 kDa
protein length
214 aa Sequence Blast
gene length
645 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

    • Gene

    Coordinates
    3,040,751 → 3,041,395

    The protein

    [SW|Domains]

  • 2 [SW|CBS domain]s (aa 7-66, aa 78-135) (according to UniProt)

    • Structure

  • [PDB|5AWE] (from Thermus thermophilus, 30% identity) [pubmed|26936147]

    • [SW|Localization]

  • Cytoplasm (Homogeneous) [Pubmed|16479537]

    • Expression and Regulation

    Operons

    genes
    [gene|17C3F56CE859BCD7A282C144C46CC92D3506ABF6|acuA]-[gene|FC9981CFB9D7EB8ACDA3032EC2F0538988F277AA|acuB]-[gene|1255813797A83D835E0656A2AAAD361B5BB2094B|acuC]
    description
    [Pubmed|7934817]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7913927], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|7913927], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • repressed by glucose (3.4-fold) ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]) [Pubmed|7913927,12850135]

    • additional information

  • the mRNA is quite stable (half-life 5 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
    • view in new tab

    Biological materials

    Mutant

  • BKE29700 (Δ[gene|FC9981CFB9D7EB8ACDA3032EC2F0538988F277AA|acuB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATTTTTTCCCCTTTGT, downstream forward: _UP4_CCGTCAGAGCAAAGGGATCT
  • BKK29700 (Δ[gene|FC9981CFB9D7EB8ACDA3032EC2F0538988F277AA|acuB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATTTTTTCCCCTTTGT, downstream forward: _UP4_CCGTCAGAGCAAAGGGATCT

    • Expression vectors

  • pGP2922 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

    • References

  • 7913927,16855235,7934817,16479537,19136592,26936147,12884008