transcriptional antiterminator of the bglP-bglH-yxiE operon and the bglS gene
function
control of beta-glucan and beta-glucoside utilization
product
transcriptional antiterminator (BglG family)
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.18|Utilization of beta-glucosides][category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.27|Utilization of other polymeric carbohydrates][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.3|PRD-type regulators][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.4|RNA binding regulators][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue][SW|Categories] containing this gene/protein
[SW|utilization of specific carbon sources], [SW|transcription factors and their control], [SW|RNA binding regulators], [SW|phosphoproteins]Gene
Coordinates on the chromosome (coding sequence)
4,012,866 -> 4,013,699
Phenotypes of a mutant
no expression of the ''[protein|search|bglP]-[protein|search|bglH]'' operonThe protein
Catalyzed reaction/ biological activity
binding to the mRNAs of ''[protein|search|bglS]'' and the ''[protein|search|bglP]-[protein|search|bglH]'' operon, causes transcription antitermination (in presence of salicin and absence of glucose)Protein family
[PRD-containing transcription factors|search|transcriptional antiterminator] BglG family of antiterminators (according to Swiss-Prot)Paralogous protein(s)
[protein|search|SacY], [protein|search|GlcT], [protein|search|SacT][SW|Domains]
N-terminal RNA binding domain [Pubmed|10610766]2 x [SW|PRD] ([SW|PTS] regulation domains) [Pubmed|11447120]Modification
phosphorylation at His-100 in [SW|PRD]-1 by phosphorylated [protein|search|BglP], inhibits LicT antitermination activityphosphorylation at His-207 and/or His-269 in [SW|PRD]-2 by His-P-[ptsH|search|HPr], stimulates LicT antitermination activityStructure
[PDB|1L1C] (complex with RAT)[PDB|1TLV] ([SW|PRD]s)[SW|Localization]
cytoplasm, even distribution in the absence of the inducer salicin, subpolar localization in the presence of salicin [Pubmed|23475962][SW|Interactions]
[protein|search|PtsH]-[protein|search|LicT], [protein|search|BglP]-[protein|search|LicT]Expression and Regulation
Operon
''[protein|search|licT]-[protein|search|bglS]'' [Pubmed|8606172][SW|Sigma factor]
[protein|search|SigA] [Pubmed|8606172]Biological materials
Mutant
GP427 (licTS, erm), available in [SW|Jörg Stülke]'s labExpression vector
for expression, purification of both PRDs in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP165, available in [SW|Jörg Stülke]'s labfor expression, purification of the RNA-binding domain in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP315, available in [SW|Jörg Stülke]'s labfor expression, purification of the RNA-binding domain in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [SW|pGP570]: pGP572, available in [SW|Jörg Stülke]'s labGFP fusion
GP1225 (spc, based on [SW|pGP1870]), available in [SW|Jörg Stülke]'s labFLAG-tag construct
GP1221 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s labLabs working on this gene/protein
[SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France[SW|Josef Deutscher], Microbiology and Molecular Genetics, INRA Paris-Grignon, France[SW|Michael Hecker], Greifswald, Germany [http://www.mikrobiologie.uni-greifswald.de/index.php?id=20 Homepage]References
Reviews
Original publications
