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scoC [Thu Aug 25 2016 17:06:39 GMT+0200 (CEST)]
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scoC [Thu Aug 25 2016 17:06:39 GMT+0200 (CEST)]

transcriptional repressor of genes expressed in the transition phase
locus
BSU09990
pI
5.00
mw
23.00 kDa
function
transition state regulator
product
transcriptional repressor (MarR family)
essential
no
synonyms
hpr, catA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.6|Transition state regulators]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

    • [SW|Categories] containing this gene/protein

  • [SW|transcription factors and their control], [SW|transition state regulators], [SW|phosphoproteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|AbrB regulon], [SW|CodY regulon], [SW|SalA regulon], [SW|SenS regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    1,073,106 -> 1,073,717

    The protein

    Modification

  • phosphorylated on Arg-3 [Pubmed|22517742]

    • Effectors of protein activity

  • activity is inhibited in an unknown way by [SW|PrkA] (possibly phosphorylation) [Pubmed|25983726]

    • Structure

  • [PDB|2FXA]

    • [SW|Interactions]

  • [protein|search|SinR]-[protein|search|ScoC]

    • Expression and Regulation

    Operon

  • ''scoC'' [Pubmed|3131303]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|19251843]

    • Regulation

  • expressed upon transition into the stationary phase ([protein|search|AbrB]) [Pubmed|2504584]

    • Regulatory mechanism

  • [protein|search|AbrB]: transcription activation [Pubmed|2504584]
  • [protein|search|SenS]: positive regulation [Pubmed|16321961]
  • [protein|search|SalA]: negative regulation [Pubmed|15126467]
  • [protein|search|CodY]: negative regulation [Pubmed|25966844]

    • Biological materials

    Mutant

  • Strain OM213 of Ogura MA, et al [Pubmed|15126467], genotype ''trpC2 scoC::pMutin-scoC leuC7'', available as [http://bgsc.org BGSC] 1A918
  • 1A918 ( ''scoC''::''erm''), [Pubmed|15126467], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A918&Search=1A918 BGSC]

    • Expression vector

  • for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

    • References

  • 3131303,16923912,1906467,10383984,16321961,14629015,1907636,15126467,2504584,15104138,19118355,19801406,19898538,20382764,22517742,23660663,11717292,25966844,19251843,26473603,25983726,26728191