SubtiBank SubtiBank
pksG
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pksG

involved in polyketide synthesis
locus
BSU_17150
pI
5.14
mw
46.62 kDa
protein length
420 aa Sequence Blast
gene length
1263 bp Sequence Blast
function
polyketide synthesis
product
hydroxymethylglutaryl CoA synthase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.1|Biosynthesis of antibacterial compounds]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.15|Biosynthesis of antibacterial compounds]

    • Gene

    Coordinates
    1,789,943 → 1,791,205

    The protein

    Catalyzed reaction/ biological activity

  • 3-oxobutanoyl-[ACP] + acetyl-[ACP] + H2O --> (3S)-hydroxy-3-methylglutaryl-[ACP] + H+ + holo-[ACP] (according to UniProt)

    • Protein family

  • [SW|thiolase-like superfamily] (according to UniProt)

    • Structure

  • [PDB|4YXQ]
  • [PDB|4YXT] (E82A mutant)
  • [PDB|4YXV] (C114A mutant)

    • Expression and Regulation

    Operons

    genes
    [gene|55C821F68B2E5F9F45CC2B2C9494D48D5C33266A|pksC]-[gene|F3E9CA9330394FD829D59B0C2D4D1EEDD2C22D3B|pksD]-[gene|CB0B856B22984DD5B10F01DD07C3961FAA742923|pksE]-[gene|6BEC9E2BCECE33803EAC14DE7E8E988FC9C8DC8B|acpK]-[gene|85D6E439529E8B3F73244A0B004B394B4FE65509|pksF]-[gene|F4C96980640CB04D3017B8EBB02918006CBCF7BB|pksG]-[gene|A4CF68F602273CFDFD55EFD2C1164F0E2E8F6972|pksH]-[gene|F437A90A97CF8ED4BA1E5CCA8A33D9E93612902B|pksI]-[gene|13EB6B903088C11D985DDB7CEC864797058FE038|pksJ]-[gene|BABBC5848F9BBF9E9318252F5AD7D1C4F55FCEAB|pksL]-[gene|40779D3A6E4F72EEFD702BF9FA15B57951783D12|pksM]-[gene|66AFF1FC7D1BBA8EA5BB57A935FF5AB890A895C1|pksN]-[gene|85213235F7D3D8E711963E53F314ED758B5FA94B|pksR]
    description
    [Pubmed|22383849]

    regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|24187085], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: activation, [Pubmed|24187085], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

    • regulation

  • expressed during the transition from growth to stationary phase ([protein|search|AbrB], [protein|search|CodY]) [Pubmed|24187085]

    • additional information

  • this is a very large operon comprising about 75 kb
    • view in new tab

    genes
    [gene|647B1D7E496E81E7FC7FD2BDCACC2486852BAC19|pksB]-[gene|55C821F68B2E5F9F45CC2B2C9494D48D5C33266A|pksC]-[gene|F3E9CA9330394FD829D59B0C2D4D1EEDD2C22D3B|pksD]-[gene|CB0B856B22984DD5B10F01DD07C3961FAA742923|pksE]-[gene|6BEC9E2BCECE33803EAC14DE7E8E988FC9C8DC8B|acpK]-[gene|85D6E439529E8B3F73244A0B004B394B4FE65509|pksF]-[gene|F4C96980640CB04D3017B8EBB02918006CBCF7BB|pksG]-[gene|A4CF68F602273CFDFD55EFD2C1164F0E2E8F6972|pksH]-[gene|F437A90A97CF8ED4BA1E5CCA8A33D9E93612902B|pksI]-[gene|13EB6B903088C11D985DDB7CEC864797058FE038|pksJ]-[gene|BABBC5848F9BBF9E9318252F5AD7D1C4F55FCEAB|pksL]-[gene|40779D3A6E4F72EEFD702BF9FA15B57951783D12|pksM]-[gene|66AFF1FC7D1BBA8EA5BB57A935FF5AB890A895C1|pksN]-[gene|85213235F7D3D8E711963E53F314ED758B5FA94B|pksR]
    description
    [pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • MGNA-A017 (pksG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/17 NBRP B. subtilis, Japan]
  • BKE17150 (Δ[gene|F4C96980640CB04D3017B8EBB02918006CBCF7BB|pksG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGCTTCTATTCCGGCTGAAA, downstream forward: _UP4_TCTGAGTTTCATCGGAAGTA
  • BKK17150 (Δ[gene|F4C96980640CB04D3017B8EBB02918006CBCF7BB|pksG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGCTTCTATTCCGGCTGAAA, downstream forward: _UP4_TCTGAGTTTCATCGGAAGTA

    • References

  • 23840410,22383849,24187085,27766092