unknown, similar to gamma-glutamyltransferase (Ggt)
Genomic Context
categories
[category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function][SW|Categories] containing this gene/protein
[SW|proteins of unknown function]This gene is a member of the following [SW|regulons]
[SW|TnrA regulon]Gene
Coordinates on the chromosome (coding sequence)
3,719,134 -> 3,720,711
The protein
Catalyzed reaction/ biological activity
(5-L-glutamyl)-peptide + an amino acid = peptide + 5-L-glutamyl amino acid (according to Swiss-Prot), however, all evidence suggests this is a misannotation [Pubmed|19935659]Protein family
gamma-glutamyltransferase family (according to Swiss-Prot), however, all evidence suggests this is a misannotation [Pubmed|19935659]Additional information
The gene is annotated in KEGG as an ortholog of gamma-glutamyltranspeptidase EC 2.3.2.2, This enzyme is necessary to utilize glutathione (GSH) as the sulfur source. No EC annotation for this gene is available in Swiss-Prot/MetaCyc. It has been shown by Minami et al. ([Pubmed|14762019]) that ywrD mutant grows well on minimal media supplied with GSH as the sole sulfur source. In addition, His-tag purified ywrD cannot hydrolyze GSH. [Pubmed|19935659]Expression and Regulation
Operon
''ywrD'' [Pubmed|12823818]Regulation
expressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|12823818]Regulatory mechanism
[protein|search|TnrA]: transcription activation [Pubmed|12823818]References
12823818,25755103,14762019 
