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mtlF [2018-07-26 15:24:50]
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mtlF [2018-07-26 15:24:50]

phosphotransferase system (PTS) mannitol-specific enzyme IIA component
locus
BSU03982
pI
4.50
mw
16.00 kDa
protein length
143 aa Sequence Blast
gene length
429 bp Sequence Blast
function
uptake of mannitol
product
phosphotransferase system(PTS) mannitol-specific enzyme IIA component
essential
no
synonyms
Outlinks
KEGGBsubCycUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.10|Utilization of mannitol]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • Gene

    Coordinates
    451,185 → 451,616

    The protein

    Structure

  • [PDB|1A3A] (from E. coli, 41% identity) [pubmed|9551558]

    • Expression and Regulation

    Operons

    genes
    [gene|5291FEF41CA547223EDF9EBAFA38D3EE550ED3F3|mtlA]-[gene|EE4A4336E25F5DCAC89214F55CDE203AB47AA249|mtlF]-[gene|4D54898D405EA25A0C74B1F02A492143D49B7B07|mtlD]
    description
    [Pubmed|12897001]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|22014119], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|1CEDC98C9EDD8F3DEA0AF4B2104DA1BF86E688C2|MtlR]: activation, [Pubmed|20444094], in [regulon|1CEDC98C9EDD8F3DEA0AF4B2104DA1BF86E688C2|MtlR regulon]

    • regulation

  • induced by mannitol ([protein|1CEDC98C9EDD8F3DEA0AF4B2104DA1BF86E688C2|MtlR]) [Pubmed|20444094]

    • additional information

  • An [SW|ncRNA|antisense RNA] is predicted for [gene|4D54898D405EA25A0C74B1F02A492143D49B7B07|mtlD] [PubMed|20525796]
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    Biological materials

    Mutant

  • BKE03982 (Δ[gene|EE4A4336E25F5DCAC89214F55CDE203AB47AA249|mtlF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03982 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACAATCACTCTCTTTC, downstream forward: _UP4_GCCATTTTCAACGAGGTGAA
  • BKK03982 (Δ[gene|EE4A4336E25F5DCAC89214F55CDE203AB47AA249|mtlF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03982 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACAATCACTCTCTTTC, downstream forward: _UP4_GCCATTTTCAACGAGGTGAA

    • References

  • 12897001,20444094,9551558