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recU [2018-04-24 15:57:12]
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recU [2018-04-24 15:57:12]

Holliday junction resolvase, DNA repair, homologous recombination and chromosome segregation; recognizes, distorts, and cleaves four-stranded recombination intermediates and modulates RecA activities, equivalent to E. coli RecU, required for efficient survival and replication restart after replication-transcription conflicts
locus
BSU22310
pI
9.51
mw
23.81 kDa
protein length
206 aa Sequence Blast
gene length
618 bp Sequence Blast
function
DNA repair/ recombination and chromosome segregation
product
Holliday junction resolvase
essential
no
synonyms
prfA,yppB,jopB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.4|Double strand breaks repair]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]

    • Gene

    Coordinates
    2,340,802 → 2,341,422

    Phenotypes of a mutant

  • the inactivations of ''[gene|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU]'' and ''[gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[gene|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]'' are synthetically lethal [Pubmed|24770420]

    • The protein

    Catalyzed reaction/ biological activity

  • binds Holliday junctions with high affinity [Pubmed|24770420]

    • Effectors of protein activity

  • [protein|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|RecU]-mediated Holliday junction resolution is stimulated by [protein|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|RuvB]-ATP [Pubmed|24770420]

    • Structure

  • [PDB|1ZP7] [Pubmed|16154091]

    • Expression and Regulation

    Operons

    genes
    [gene|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU]-[gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA]
    description
    [Pubmed|7814321]

    sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|22211522], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulation

  • constitutive during vegetative growth [Pubmed|15758244]
    • view in new tab

    Biological materials

    Mutant

  • GP891 (''recU''::''cat''), available in [SW|Jörg Stülke]'s lab
  • BKE22310 ( ''recU''::''ermC''), (available at the [http://www.bgsc.org/ BGSC] and in [SW|Fabian Commichau]'s lab) [pubmed|28189581]
  • BP784 ( ''recU''::''ermC''), (available in [SW|Fabian Commichau]'s lab)
  • 1A895 (no resistance), [Pubmed|16020779], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A895&Search=1A895 BGSC]
  • BKE22310 (Δ[gene|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTCTCATCCTCCTTAT, downstream forward: _UP4_TGATTAACGAAAGGTTGAGA
  • BKK22310 (Δ[gene|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTCTCATCCTCCTTAT, downstream forward: _UP4_TGATTAACGAAAGGTTGAGA

    • References

    Reviews

  • 22933559,9442895

    • Original publications

  • 9642195,16024744,15317759,11810266,19730681,19422832,7814321,18179421,16154091,21600217,18684995,16020779,24770420,24792171,14701911,25939832,27903910

    • RecU in other organisms

  • 23356868