Holliday junction resolvase, DNA repair, homologous recombination and chromosome segregation; recognizes, distorts, and cleaves four-stranded recombination intermediates and modulates RecA activities, equivalent to E. coli RecU, required for efficient survival and replication restart after replication-transcription conflicts
function
DNA repair/ recombination and chromosome segregation
product
Holliday junction resolvase
synonyms
recG, prfA, yppB, jopB
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination][category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.4|Double strand breaks repair][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)][SW|Categories] containing this gene/protein
[SW|DNA repair/ recombination], [SW|cell envelope stress proteins (controlled by SigM, V, W, X, Y)]This gene is a member of the following [SW|regulons]
[SW|SigM regulon]Gene
Coordinates on the chromosome (coding sequence)
2,340,802 -> 2,341,422
Phenotypes of a mutant
the inactivations of ''[protein|search|recU]'' and ''[protein|search|ruvA]-[protein|search|ruvB]'' are synthetically lethal [Pubmed|24770420]The protein
Catalyzed reaction/ biological activity
binds Holliday junctions with high affinity [Pubmed|24770420]Effectors of protein activity
[protein|search|RecU]-mediated Holliday junction resolution is stimulated by [protein|search|RuvB]-ATP [Pubmed|24770420]Structure
[PDB|1ZP7] [Pubmed|16154091][SW|Interactions]
form dimers [Pubmed|21600217][protein|search|RecA]-[protein|search|RecU] [Pubmed|16024744][protein|search|RecU]-[protein|search|RuvB] [Pubmed|24770420,21600217]formation of a ternary complex [protein|search|RecU]-[protein|search|RuvB]-Holliday junction DNA [Pubmed|24770420]Expression and Regulation
Operon
''[protein|search|recU]-[protein|search|ponA]'' [Pubmed|7814321][SW|Sigma factor]
[protein|search|SigM] [Pubmed|18179421]Biological materials
Mutant
GP891 (''recU''::''cat''), available in [SW|Jörg Stülke]'s lab1A895 (no resistance), [Pubmed|16020779], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A895&Search=1A895 BGSC]References
Reviews
Original publications
9642195,16024744,15317759,11810266,19730681,19422832,7814321,18179421,16154091,21600217,18684995,16020779,24770420,24792171,14701911,25939832,27903910 RecU in other organisms
