SubtiBank SubtiBank
yobH
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yobH

C-terminal fragment of the Y family DNA polymerase [protein|A04F698C8836E84991BC875BCB7B1460D8E74A8E|YozK]-[protein|ED0A85A8A5FCD12C99507EB033F88586521A7C47|YobH]
locus
BSU_18930
pI
10.00
mw
23.06 kDa
protein length
217 aa Sequence Blast
gene length
655 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProt

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.6|DNA repair/ recombination/ based on similarity]

    • The protein

    Protein family

  • [SW|DNA polymerase type-Y family] (according to UniProt)

    • [SW|Domains]

  • [SW|UmuC domain] (aa 1-68) (according to UniProt)

    • Structure

  • [PDB|4DEZ] (from Mycobacterium smegmatis, 24% identity) [pubmed|22868761]

    • Expression and Regulation

    Operons

    genes
    [gene|94F2146AC6998CC77CD2E86CE8BE8DC98AEBB9CD|yozL]-[gene|A04F698C8836E84991BC875BCB7B1460D8E74A8E|yozK]-[gene|ED0A85A8A5FCD12C99507EB033F88586521A7C47|yobH]
    description
    [Pubmed|16267290]

    regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [Pubmed|16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]

    • regulation

  • expression during spore [SW|germination] is strongly reduced under conditions of osmotic stress [Pubmed|27766092]
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    Biological materials

    Mutant

  • MGNA-A306 (yobH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/306 NBRP B. subtilis, Japan]
  • BKE18930 (Δ[gene|ED0A85A8A5FCD12C99507EB033F88586521A7C47|yobH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTCAGGATTCTCTT, downstream forward: _UP4_TGAGAAGTATCTCAGTTACG
  • BKK18930 (Δ[gene|ED0A85A8A5FCD12C99507EB033F88586521A7C47|yobH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTCAGGATTCTCTT, downstream forward: _UP4_TGAGAAGTATCTCAGTTACG

    • References

  • 16267290,27766092,22868761,30916324