SubtiBank SubtiBank
yvcJ [2019-07-12 08:48:44]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

yvcJ [2019-07-12 08:48:44]

GTPase, nucleotide-binding protein, similar to RNase adaptor protein
locus
BSU34770
pI
6.70
mw
33.70 kDa
protein length
295 aa Sequence Blast
gene length
888 bp Sequence Blast
function
required for the expression of late competence genes
product
GTPase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]

    • Gene

    Coordinates
    3,571,501 → 3,572,388

    Phenotypes of a mutant

  • reduced [SW|genetic competence] [Pubmed|19074378]

    • The protein

    Catalyzed reaction/ biological activity

  • binds and hydrolyzes nucleotides, required for the expression of late competence genes (''[gene|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK], [gene|4D76F07D6124D56C7CEBBDAB9E84B2718BF1E7FC|comS]'') [Pubmed|19074378]

    • Protein family

  • UPF0042 family (according to Swiss-Prot)

    • Structure

  • [PDB|5O5Q] (RapZ from E. coli, 41% identity) [pubmed|28977623]

    • [SW|Localization]

  • can be localized in the cell either as a helical-like pattern or as foci close to the poles and the septa depending on growth phase and on growth medium [Pubmed|21709426]

    • Additional information

  • [http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=retrieve&dopt=full_report&list_uids=947727&log$=genesensor5&logdbfrom=pubmed Information on the ''E. coli'' homolog]

    • Expression and Regulation

    Operons

    genes
    [gene|0C40455EB53D25363FC3EB0E84502A76520F5F91|nahA]-[gene|EB12C6B4F36B57BF0C59CE048CBAE18734C78D35|yvcJ]-[gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR]-[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA]-[gene|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh]-[gene|C521BEE1EEEA7AD283A3C47C34301A8603CD9188|yvcN]
    description
    [Pubmed|9237995]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16272399], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • constitutive expression at both protein and RNA levels [Pubmed|24097947,22383849]

    • additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|yvcI]' and '[protein|search|trxB]' [PubMed|20525796]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B641 (yvcJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1640 NBRP B. subtilis, Japan]
  • 1A885 ( ''yvcJ''::''cat''), [Pubmed|19074378], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A885&Search=1A885 BGSC]
  • BKE34770 (Δ[gene|EB12C6B4F36B57BF0C59CE048CBAE18734C78D35|yvcJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTCTCCCCCCTGACT, downstream forward: _UP4_ATTGAAAAGAGAAGCCGGAA
  • BKK34770 (Δ[gene|EB12C6B4F36B57BF0C59CE048CBAE18734C78D35|yvcJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTCTCCCCCCTGACT, downstream forward: _UP4_ATTGAAAAGAGAAGCCGGAA

    • Expression vectors

  • pGP735 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Stülke] lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW| Görke] lab

    • labs

  • [SW|Anne Galinier], University of Marseille, France

    • References

    Original publications

  • 9237995,16272399,19074378,21709426

    • Publications on the ''E. coli'' homolog

  • 17824929,28977623,24667238,23475961