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ybdK [2019-08-28 09:24:40]
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ybdK [2019-08-28 09:24:40]

two-component sensor kinase
locus
BSU_02010
pI
5.21
mw
37.12 kDa
protein length
320 aa Sequence Blast
gene length
963 bp Sequence Blast
function
unknown
product
two-component sensor kinase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • Gene

    Coordinates
    221,950 → 222,912

    The protein

    Catalyzed reaction/ biological activity

  • autophosphorylation, phosphorylation of [protein|5F6D14A0B360832F9FD470668B006A628B109A56|YbdJ]
  • ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

    • [SW|Domains]

  • two transmembrane segments, C-terminal histidine phosphotransferase domain

    • Modification

  • autophosphorylation on a His residue

    • [SW|Localization]

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|EA36C28A990EFFAEBFE279467A0B56B6B5F5255D|ybdK]
    description
    [http://dbtbs.hgc.jp/COG/prom/ybdK.html DBTBS]

    regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: positive regulation, in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]
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    Biological materials

    Mutant

  • MGNA-B957 (ybdK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1956 NBRP B. subtilis, Japan]
  • BKE02010 (Δ[gene|EA36C28A990EFFAEBFE279467A0B56B6B5F5255D|ybdK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTGATATTTTGTCTTGA, downstream forward: _UP4_TGACCCCGCTGATGTTTTTC
  • BKK02010 (Δ[gene|EA36C28A990EFFAEBFE279467A0B56B6B5F5255D|ybdK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTGATATTTTGTCTTGA, downstream forward: _UP4_TGACCCCGCTGATGTTTTTC

    • References

  • 10094672,11948146,20035725