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walK
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walK

two-component sensor kinase, controls cell wall metabolism, co-ordinates cell wall remodelling with [SW|cell division] by controlling the transcription of genes for autolysins and their inhibitors
locus
BSU_40400
pI
4.78
mw
69.86 kDa
protein length
611 aa Sequence Blast
gene length
1836 bp Sequence Blast
function
control of cell wall metabolism
product
two-component sensor kinase
essential
yes
ec
2.7.13.3
synonyms
yycG
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.5|Cell wall/ other]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • Gene

    Coordinates
    4,151,853 → 4,153,688

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • essentiality can be bypassed by constitutive expression of [gene|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|lytE] or [gene|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|cwlO] in a [gene|603E226CE488A25C4E28A6A7363CCD65BE64BB21|pdaC] [gene|0CA55371306D4BD768CFA82027DCB4D581BCCB87|iseA] mutant background [pubmed|29465029]
  • a mutant (point mutation) has been selected during growth at low pressure [Pubmed|26296725]

    • The protein

    Catalyzed reaction/ biological activity

  • autophosphorylation, phosphorylation of [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR]
  • ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

    • Protein family

  • two-component sensor kinase

    • Paralogous protein(s)

  • [protein|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|ResE], [protein|C81F68521F17861D50926D7D8757DC1D2340BC0D|PhoR]

    • [SW|Domains]

  • two transmembrane segments
  • [SW|HAMP domain] (aa 204-256) (according to UniProt)
  • [SW|PAS domain] (aa 263-324) (according to UniProt)
  • [SW|PAC domain] (aa 325-379) (according to UniProt)
  • [SW|Histidine kinase domain] (aa 383-602) (according to UniProt)

    • Modification

  • autophosphorylation on a His residue

    • Effectors of protein activity

  • activity is modulated in response to changes in D,L-endopeptidase activity (via the extracellular [SW|PAS domain]) [pubmed|31808740]

    • Structure

  • [PDB|3SL2] (complex of the ATP binding histidine kinase domain with ATP) [Pubmed|22751669]

    • [SW|Localization]

  • cell membrane [Pubmed|21219466]
  • part of the division septum in growing cells, interacts with the [SW|divisome] [Pubmed|18573169]

    • Expression and Regulation

    Operons

    genes
    [gene|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]-[gene|E88978809272FAC2520DB4ABCA0554F8028F3451|walK]-[gene|06D2D03C65D50E3909B2C93835193815B6016967|walH]-[gene|EE681C9A42FCC29CBB55CC9566EE4CFF986D0F1D|walI]-[gene|D5321F707E183E72B225F1CED2F2FDCB1183B88A|walJ]-[gene|75B8C37E589C855D2BAD7FB51B9600E161ACFBD3|htrC]
    description
    [Pubmed|9829949]

    regulation

  • ''htrC'' transcript expressed during sporulation [Pubmed|9829949]
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    Biological materials

    Mutant

  • MGNA-B827 (yycG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1826 NBRP B. subtilis, Japan]

    • Expression vectors

  • pGP2636, constitutive expression in ''B. subtilis'' (based on [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 19019149,18792692,10094672,18245295,28886686,31976860

    • Original Publications

  • 18573169,17350627,17827301,9829949,17581128,10878122,16030236,12950927,18408157,19342776,20057515,20167622,21219466,23632918,22751669,22526318,24490131,24900578,26296725,28674376,29465029,29465029,31339578,31808740