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citR [2017-08-04 14:59:48]
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citR [2017-08-04 14:59:48]

transcriptional repressor of citA
locus
BSU09430
pI
8.67
mw
35.44 kDa
protein length
308 aa Sequence Blast
gene length
924 bp Sequence Blast
function
regulation of the minor citrate synthase
product
transcriptional repressor (LysR family)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

    • Gene

    Coordinates
    1,020,073 → 1,020,948

    The protein

    Protein family

  • [SW|LysR family]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|citR]
    description
    [Pubmed|8045898]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8045899], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
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    Biological materials

    Mutant

  • GP1283 (''[gene|6684F6083B001E9FDC5967799B715AE966B83E1D|citA]''::''aphA3''), available in [SW|Jörg Stülke]'s lab
  • GP1753 (''[gene|E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|citR] [gene|6684F6083B001E9FDC5967799B715AE966B83E1D|citA]''::''aphA3''), the kanamycin resistance cassette can be cut out by introducing the ''cre''-rekombinase into the chromosom of ''B. subtilis'', available in [SW|Jörg Stülke]'s lab
  • GP2360 (''[gene|E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|citR] [gene|6684F6083B001E9FDC5967799B715AE966B83E1D|citA]''::''erm''), the kanamycin resistance cassette can be cut out by introducing the ''cre''-rekombinase into the chromosom of ''B. subtilis'', available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • pGP2264 (N-terminal His-tag, purification from ''E. coli'', in [SW|pETM-11]), available in [SW|Jörg Stülke]'s lab

    • References

  • 8045898,8045899