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pgi [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]
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pgi [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

glucose 6-phosphate isomerase, glycolytic / gluconeogenic enzyme
locus
BSU31350
pI
4.00
mw
50.00 kDa
function
enzyme in glycolysis / gluconeogenesis
product
glucose-6-phosphate isomerase
essential
no
ec
5.3.1.9
synonyms
yugL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.1|Glycolysis]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.2|Gluconeogenesis]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.9|Newly identified competence genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]

    • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism], [SW|phosphoproteins], [SW|most abundant proteins]

    • Gene

    Coordinates on the chromosome (coding sequence)
    3,220,731 -> 3,222,083

    The protein

    Catalyzed reaction/ biological activity

  • D-glucose 6-phosphate = D-fructose 6-phosphate (according to Swiss-Prot) D-glucose 6-phosphate = D-fructose 6-phosphate

    • Protein family

  • GPI family (according to Swiss-Prot) GPI family

    • Modification

  • phosphorylation on Thr-39 [Pubmed|17218307]

    • Effectors of protein activity

  • competitively inhibited by 6-phosphogluconate (in ''B.caldotenax, B.stearothermophilus'') [Pubmed|7400125]

    • Structure

  • [PDB|1B0Z] (''Geobacillus stearothermophilus''), [http://www.proteopedia.org/wiki/index.php/2pgi 2PGI] (''Geobacillus stearothermophilus'')

    • [SW|Localization]

  • cytoplasm (homogeneously distributed throughout the cell) [Pubmed|24825009]

    • Additional information

  • extensive information on the structure and enzymatic properties of Pgi can be found at [http://www.proteopedia.org/wiki/index.php/Phosphoglucose_isomerase Proteopedia]

    • Expression and Regulation

    Operon

  • ''[protein|search|pgi]-[protein|search|yugM]-[protein|search|yugN]'' [Pubmed|11489127]

    • Regulation

  • constitutively expressed [Pubmed|11489127]

    • Additional information

  • belongs to the 100 [[most abundant proteins]] [PubMed|15378759]

    • Biological materials

    Mutant

  • GP508 (spc), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
  • GP1746 (''pgi::aphA3''), available in [SW|Jörg Stülke]' lab

    • Expression vector

  • pGP398 (N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • pGP510 (in [protein|search|pAC6]), available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html homepage]

    • References

  • 17218307,19052382,4214896,23420519,11489127,4275311,11491085,17218307,24571712,15378759,24825009