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bceB [2019-03-31 15:31:49]
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bceB [2019-03-31 15:31:49]

primary bacitracin resistance determinant, ABC transporter (permease) for the export of bacitracin, plectasin, mersacidin and actagardine, sensory component of the Bce regulatory & detoxification system
locus
BSU30370
pI
9.79
mw
72.01 kDa
protein length
646 aa Sequence Blast
gene length
1941 bp Sequence Blast
function
export of toxic peptides
product
bacitracin ABC transporter (permease)
essential
no
synonyms
ytsD
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.2|Exporters] → [category|SW 1.2.1.2.3|Export of peptides]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,109,397 → 3,111,337

    Phenotypes of a mutant

  • 85-fold increased sensitivity to bacitracin [Pubmed|26815905]

    • The protein

    Catalyzed reaction/ biological activity

  • binds bacitracin, KD: 60 nM [Pubmed|25118291]
  • inhibits [protein|08F24CA21DC54031F6158AC0C772F3B520E5A849|BceS] autophosphorylation activity in the absence of bacitracin [Pubmed|25118291]

    • Protein family

  • ABC-4 integral membrane protein family (according to Swiss-Prot)

    • Effectors of protein activity

  • the activity of the [protein|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|BceA]-[protein|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|BceB] [SW|ABC transporter] is inhibited by heptaprenyl pyrophosphate which accumulates in a ''[gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB]'' mutant [Pubmed|24806199]

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|bceA]-[gene|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|bceB]
    description
    [Pubmed|12890034]

    regulatory mechanism

  • [protein|C239C155F5452C86BF6C78190BABBBB07A63DF87|BceR]: activation, [Pubmed|12890034], in [regulon|C239C155F5452C86BF6C78190BABBBB07A63DF87|BceR regulon]

    • regulation

  • induced in the presence of bacitracin, plectasin, mersacidin and actagardine, already at very low levels of bacitracin ([protein|search|BceR]) [Pubmed|12890034,26815905]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A168 (ytsD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/168 NBRP B. subtilis, Japan]
  • BKE30370 (Δ[gene|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|bceB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATTAATGTTCATGCTGCAC, downstream forward: _UP4_TGAAACAGAAAAAGCTCCAG
  • BKK30370 (Δ[gene|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|bceB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATTAATGTTCATGCTGCAC, downstream forward: _UP4_TGAAACAGAAAAAGCTCCAG

    • References

    Reviews

  • 27344142,28152228,29404338

    • Original publications

  • 18394148,17905982,12890034,14651641,10092453,14612242,21283517,20606066,21078927,23687272,25118291,24806199,26199330,26815905