SubtiBank SubtiBank
yfnE
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yfnE

similar to chondroitin synthase
locus
BSU_07300
pI
5.96
mw
45.33 kDa
protein length
392 aa Sequence Blast
gene length
1179 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

    • Gene

    Coordinates
    801,172 → 802,350

    The protein

    Protein family

  • [SW|glycosyltransferase 2 family] (according to UniProt)

    • Structure

  • [PDB|2Z86] (E. coli chondroitin synthase, corresponds to aa 4 ... 259, 25% identity) [pubmed|18771653]

    • Expression and Regulation

    Operons

    genes
    [gene|B25AE6979AF12628780C4489FFED966C6B31968A|yfnH]-[gene|9836E90BD443F5269A43E5C69FCF2CCB22EDF65B|yfnG]-[gene|4148A3F8E256D9B1D667C28428D867B5B6B5F3CE|yfnF]-[gene|E3481379D3FEC02C35CB4BE17A5246A208C5C86D|yfnE]-[gene|1D0286ECDA32FB21737170B54A57D3FE482C6422|yfnD]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15383836], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]
  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, internal promoter in [protein|E3481379D3FEC02C35CB4BE17A5246A208C5C86D|YfnE] open reading frame [Pubmed|15383836], see [http://dbtbs.hgc.jp/COG/prom/yfnHGFED.html DBTBS] for details, in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

    • regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: activation, [Pubmed|26577401], in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]

    • regulation

  • expressed during sporulation ([protein|search|SigK]) [Pubmed|15383836]
    • view in new tab

    genes
    [gene|E3481379D3FEC02C35CB4BE17A5246A208C5C86D|yfnE]-[gene|1D0286ECDA32FB21737170B54A57D3FE482C6422|yfnD]
    description
    [Pubmed|22383849]

    regulation

  • expressed during sporulation ([protein|search|SigK]) [Pubmed|15383836]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-C229 (yfnE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2227 NBRP B. subtilis, Japan]
  • BKE07300 (Δ[gene|E3481379D3FEC02C35CB4BE17A5246A208C5C86D|yfnE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTCATTTTCACTTCCTG, downstream forward: _UP4_CTTAGATCATTGGATGTGGT
  • BKK07300 (Δ[gene|E3481379D3FEC02C35CB4BE17A5246A208C5C86D|yfnE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTCATTTTCACTTCCTG, downstream forward: _UP4_CTTAGATCATTGGATGTGGT

    • References

  • 15383836,18771653