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hag [Mon May 16 2016 15:51:48 GMT+0200 (CEST)]
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hag [Mon May 16 2016 15:51:48 GMT+0200 (CEST)]

flagellin protein, about 20,000 subunits make up one flagellum
locus
BSU35360
pI
4.00
mw
32.00 kDa
function
motility and chemotaxis
product
flagellin protein
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.2|Flagellar proteins]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.6|Other proteins required for efficient pellicle biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.4|Swarming]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

    • [SW|Categories] containing this gene/protein

  • [SW|motility and chemotaxis], [SW|most abundant proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|CodY regulon], [SW|CsrA regulon], [SW|ScoC regulon], [SW|SigD regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    3,634,987 -> 3,635,901

    Phenotypes of a mutant

  • no swarming motility on B medium. [Pubmed|19202088]
  • not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation [Pubmed|26122431]
  • few hours delay in pellicle development [Pubmed|26122431]
  • mutant has increased fitness in planktonic culture when competed with the wild-type NCIB3610 strain [Pubmed|26122431]

    • The protein

    Protein family

  • bacterial flagellin family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|search|YvzB] (C-terminal domain of Hag)

    • Structure

  • [PDB|3A5X] (from ''Salmonella typhimurium'', 42% identity)

    • [SW|Localization]

  • extracellular (no signal peptide), major component of the secretome [Pubmed|18957862]
  • membrane [Pubmed|18763711]

    • [SW|Interactions]

  • [protein|search|FlhA]-[protein|search|Hag] [Pubmed|20534509]
  • [protein|search|FlhA]-([protein|search|FliS]-[protein|search|Hag]) [Pubmed|20534509]
  • [protein|search|FliS]-[protein|search|Hag] [Pubmed|20534509]
  • [protein|search|FliW]-[protein|search|Hag] [Pubmed|21895793]
  • [SW|Hag]-[SW|FliD] [Pubmed|26490009]

    • Expression and Regulation

    Operon

  • ''[protein|search|hag]'' [Pubmed|2498284]

    • [SW|Sigma factor]

  • [protein|search|SigD] [Pubmed|2498284]

    • Regulation

  • repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12730172]

    • Regulatory mechanism

  • [protein|search|CodY]: transcription repression [Pubmed|12730172]
  • [protein|search|CsrA]: translation inhibition [Pubmed|17555441]
  • [protein|search|ScoC]: transcription repression [Pubmed|19118355]

    • Additional information

  • the ''[protein|search|hag]'' gene is strongly overexpressed in a ''[protein|search|ymdB]'' mutant (loss of bistable gene expression) [PubMed|21856853]
  • belongs to the 100 [[most abundant proteins]] [PubMed|15378759]

    • Biological materials

    Mutant

  • GP901 (aphA3), GP902 (tet) [Pubmed|21856853], both available in [SW|Jörg Stülke]'s lab
  • 1A915 ( ''hag''::''cat''), [Pubmed|19202088], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A915&Search=1A915 BGSC]
  • 1A783 ( ''hag''::''erm''), [Pubmed|2174860], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A783&Search=1A783 BGSC]
  • 1A842 ( ''hag''::''kan''), [Pubmed|14762011], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A842&Search=1A842 BGSC]
  • DS1677 (marker-less in NCIB3610) [Pubmed|18566286]

    • Expression vector

  • expression in ''B. subtilis'', in [SW|pBQ200]: pGP1089, available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • pGP1035 (in [protein|search|pAC6]), pGP755 (in [protein|search|pAC7]), there is also a series of promoter deletion variants in [protein|search|pAC6] and [protein|search|pAC7] [Pubmed|21856853], all available in [SW|Jörg Stülke]'s lab

    • GFP fusion

  • BP494 (bglS:: (hag-promoter-cfp-aphA3)), BP496 (amyE:: (hag-promoter-iyfp-cat)), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Daniel Kearns]

    • References

    Reviews

  • 22672726,25251856,26490009

    • Original Publications

  • 18566286,26122431,23144244,21602220,21856853,2498284,9648743,9096206,7708689,19202088,18763711,21895793,10809682,19118355,17555441,20534509,15378759