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prkD [2019-08-15 13:17:06]
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prkD [2019-08-15 13:17:06]

protein kinase D
locus
BSU_02030
pI
6.36
mw
29.91 kDa
protein length
256 aa Sequence Blast
gene length
771 bp Sequence Blast
function
unknown
product
protein kinase D
essential
no
ec
2.7.11.1
synonyms
ybdM
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]

    • Gene

    Coordinates
    223,219 → 223,989

    The protein

    Catalyzed reaction/ biological activity

  • ATP + L-seryl-[protein] --> ADP + H+ + O-phospho-L-seryl-[protein] (according to UniProt)
  • ATP + L-threonyl-[protein] --> ADP + H+ + O-phospho-L-threonyl-[protein] (according to UniProt)
  • phosphorylates [protein|047CE33C0CC127DAD15D68D63843DF89F93ED3BF|DegS] on Ser-76 (in vitro) [Pubmed|21304896]

    • Protein family

  • [SW|protein kinase superfamily] (according to UniProt)
  • [SW|Ser/Thr protein kinase family] (according to UniProt)

    • Modification

  • autophosphorylated on Thr-174 (''in vitro'') [Pubmed|20389117]

    • Structure

  • [PDB|4EQM] (PknB from Staphylococcus aureus, 34% identity in the N-terminal part, aa 18 ... 174) [pubmed|22701750]

    • Biological materials

    Mutant

  • MGNA-B958 (ybdM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1957 NBRP B. subtilis, Japan]
  • GP578 (aphA3), available in [SW|Jörg Stülke]'s lab
  • BKE02030 (Δ[gene|E0097A00C82136BB0B1FB89FBA177E28A4EC3D54|prkD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTGCACTTCCCTTGG, downstream forward: _UP4_CGCGAGGATCTAAACCGGGC
  • BKK02030 (Δ[gene|E0097A00C82136BB0B1FB89FBA177E28A4EC3D54|prkD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTGCACTTCCCTTGG, downstream forward: _UP4_CGCGAGGATCTAAACCGGGC

    • Expression vectors

  • pGP390 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab
  • for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP821, available in [SW|Jörg Stülke]'s lab, [pubmed|20389117]
  • for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP1407, available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • pGP830 (in [SW|pAC7]), available in [SW|Jörg Stülke]'s lab

    • References

  • 20389117,12406230,21304896,24731262,25278935,22701750