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ysxC [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]
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ysxC [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]

assembly of the 50S subunit of the ribosome
locus
BSU28190
pI
9.00
mw
21.00 kDa
function
ribosome assembly
product
GTPase
essential
yes
synonyms
orfX
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.6|Ribosome assembly]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.3|GTP-binding proteins]

    • [SW|Categories] containing this gene/protein

  • [SW|translation], [SW|essential genes], [SW|membrane proteins], [SW|GTP-binding proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|CtsR regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,879,882 -> 2,880,469

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • participates in the maturation of the central protuberance, GTPase associated region and key RNA helices in the A, P and E functional sites of the 50S subunit [Pubmed|27484475]

    • Protein family

  • engB family (according to Swiss-Prot) Era/Obg family

    • Paralogous protein(s)

  • [protein|search|Era], [protein|search|Obg], [protein|search|YphC], [protein|search|RbgA], [protein|search|YqeH], [protein|search|ThdF], [protein|search|YyaF], [protein|search|YnbA]

    • Structure

  • [PDB|1SVI] (complex with GDP), [PDB|1SUL]

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot)

    • [SW|Interactions]

  • [protein|search|YsxC]-[protein|search|RplL] [Pubmed|17981968]
  • [protein|search|YsxC]-[protein|search|RplF] [Pubmed|17981968]
  • [protein|search|YsxC]-[protein|search|RplC] [Pubmed|17981968]
  • [protein|search|YsxC]-[protein|search|RplA] [Pubmed|17981968]

    • Expression and Regulation

    Operon

  • ''[protein|search|lonA]-[protein|search|ysxC]'' [Pubmed|7961402]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|7961402]

    • Regulation

  • induced by heat stress ([protein|search|CtsR]) [Pubmed|9852015]

    • Regulatory mechanism

  • [protein|search|CtsR]: transcription repression [Pubmed|9852015]

    • Biological materials

    Expression vector

  • for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP834, available in [SW|Stülke] lab
  • for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP842, available in [SW|Stülke] lab

    • Labs working on this gene/protein

  • [SW|Naotake Ogasawara], Nara, Japan

    • References

    Reviews

  • 19575570,21885683

    • Original publications

  • 17981968,11073929,12427945,16997968,7961402,9852015,14684918,15136032,21636901,25771857,26102633,27484475