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amiE [2019-07-22 08:56:45]
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amiE [2019-07-22 08:56:45]

N-acetylmuramyl-L-alanine amidase
locus
BSU_01670
pI
9.35
mw
49.14 kDa
protein length
441 aa Sequence Blast
gene length
1326 bp Sequence Blast
function
recycling of cell wall material
product
N-acetylmuramyl-L-alanine amidase
essential
no
synonyms
ybbE
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.3|Utilization of cell wall components]

    • Gene

    Coordinates
    188,408 → 189,733

    The protein

    Catalyzed reaction/ biological activity

  • hydrolyzes the amide bond between MurNAc and the L-alanine residue of the stem peptide [Pubmed|20400549]

    • Protein family

  • peptidase S12 family (single member, according to UniProt)

    • Structure

  • [PDB|4IVK] (from uncultured bacterium, 25% identity) [pubmed|23737193]

    • Expression and Regulation

    Operons

    genes
    [gene|0B1F43F43A4DC1574A153DBFBAA5D0A1855F9B61|murQ]-[gene|125872506C400CB6B3DAEA8B5130B064DFBA4494|murR]-[gene|178D5E2AA1225FE909E6F2B63B3595F5ABB8E7A2|murP]-[gene|DEACFD435DB01C4B3ADEF397BEE08E2E7C7FCC36|amiE]-[gene|A5D4BF262BD365DCEBA9358C8DB295E605DE4D84|nagZ]-[gene|005D37F34CA3D6891F7D8FF7A2AFA1CBCB556FFE|ybbC]
    description
    [Pubmed|22383849]

    regulatory mechanism

  • [protein|125872506C400CB6B3DAEA8B5130B064DFBA4494|MurR]: repression, [pubmed|30038046], in [regulon|125872506C400CB6B3DAEA8B5130B064DFBA4494|MurR regulon]

    • regulation

  • expressed in late exponential and early stationary phase [Pubmed|20400549]
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    Biological materials

    Mutant

  • BKE01670 (Δ[gene|DEACFD435DB01C4B3ADEF397BEE08E2E7C7FCC36|amiE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGGCTAGCCCCTTTCTG, downstream forward: _UP4_TGAATGATTAGACGATAGGA
  • BKK01670 (Δ[gene|DEACFD435DB01C4B3ADEF397BEE08E2E7C7FCC36|amiE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGGCTAGCCCCTTTCTG, downstream forward: _UP4_TGAATGATTAGACGATAGGA

    • References

  • 10627040,20400549,22383849,23737193,30038046