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amiE [2018-05-18 16:59:45]
N-acetylmuramyl-L-alanine amidase
function
recycling of cell wall material
product
N-acetylmuramyl-L-alanine amidase
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.3|Utilization of cell wall components]Gene
Coordinates
188,408 → 189,733
The protein
Catalyzed reaction/ biological activity
hydrolyzes the amide bond between MurNAc and the L-alanine residue of the stem peptide [Pubmed|20400549]Protein family
UPF0214 family (according to Swiss-Prot)Structure
[PDB|4IVK] (25% identity)Expression and Regulation
Operons
genes
[gene|0B1F43F43A4DC1574A153DBFBAA5D0A1855F9B61|murQ]-[gene|125872506C400CB6B3DAEA8B5130B064DFBA4494|murR]-[gene|178D5E2AA1225FE909E6F2B63B3595F5ABB8E7A2|murP]-[gene|DEACFD435DB01C4B3ADEF397BEE08E2E7C7FCC36|amiE]-[gene|A5D4BF262BD365DCEBA9358C8DB295E605DE4D84|nagZ]-[gene|005D37F34CA3D6891F7D8FF7A2AFA1CBCB556FFE|ybbC]
description
[Pubmed|22383849]
regulatory mechanism
[protein|125872506C400CB6B3DAEA8B5130B064DFBA4494|MurR]: repression, [pubmed|30038046], in [regulon|125872506C400CB6B3DAEA8B5130B064DFBA4494|MurR regulon]regulation
expressed in late exponential and early stationary phase [Pubmed|20400549]view in new tabBiological materials
Mutant
BKE01670 (Δ[gene|DEACFD435DB01C4B3ADEF397BEE08E2E7C7FCC36|amiE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGGCTAGCCCCTTTCTG, downstream forward: _UP4_TGAATGATTAGACGATAGGABKK01670 (Δ[gene|DEACFD435DB01C4B3ADEF397BEE08E2E7C7FCC36|amiE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGGCTAGCCCCTTTCTG, downstream forward: _UP4_TGAATGATTAGACGATAGGAReferences
10627040,20400549,22383849 
