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skfA [2018-07-17 19:15:26]
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skfA [2018-07-17 19:15:26]

spore killing factor
locus
BSU01910
pI
10.65
mw
5.80 kDa
protein length
55 aa Sequence Blast
gene length
165 bp Sequence Blast
function
killing of sister cells
product
spore killing factor
essential
no
synonyms
ybcO
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.17|Toxins, antitoxins and immunity against toxins]
  • [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.4|Prophage 1]

    • Gene

    Coordinates
    213,941 → 214,108

    The protein

    Modification

  • the mature SKF is cyclic 26-amino acid peptide that is posttranslationally modified with one disulfide and one cysteine thioether bridged to the alpha-position of a methionine [Pubmed|20805502], the thioether bond is generated by [protein|F981C605C652D1A4429579A5F24608CE7F570834|SkfB] [Pubmed|23282011]

    • [SW|Localization]

  • secreted (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|DDB0022F50999AC52809D651C9CC5A5FDC71302C|skfA]-[gene|F981C605C652D1A4429579A5F24608CE7F570834|skfB]-[gene|4DFF9924E07D834F37580D73E88451C1671C5111|skfC]-[gene|5886364199844356446C08F08664B301A714E74A|skfE]-[gene|77D93CAEF46EBBB9BAF570714445E194D5F2884E|skfF]-[gene|527AB8EAAF0A1AAD4E5B714B35ED9F78BECC17EC|skfG]-[gene|F31B12F80AFC3580FD33811A1C22E74F75DC1A85|skfH]
    description
    [Pubmed|16816204]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16452424], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [PubMed|14651647,15687200], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [PubMed|15687200,17720793], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: activation, [Pubmed|16816204], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]

    • regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647,15687200 PubMed]

    • additional information

  • Northern blotting during during phosphate limitation showed an intense 0.25 kb '[protein|DDB0022F50999AC52809D651C9CC5A5FDC71302C|SkfA]'-specific transcript, and a weaker 6.5 kb [gene|DDB0022F50999AC52809D651C9CC5A5FDC71302C|skfA]-[gene|F981C605C652D1A4429579A5F24608CE7F570834|skfB]-[gene|4DFF9924E07D834F37580D73E88451C1671C5111|skfC]-[gene|5886364199844356446C08F08664B301A714E74A|skfE]-[gene|77D93CAEF46EBBB9BAF570714445E194D5F2884E|skfF]-[gene|527AB8EAAF0A1AAD4E5B714B35ED9F78BECC17EC|skfG]-[gene|F31B12F80AFC3580FD33811A1C22E74F75DC1A85|skfH] transcript
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    Biological materials

    Mutant

  • MGNA-B971 (ybcO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1970 NBRP B. subtilis, Japan]
  • BKE01910 (Δ[gene|DDB0022F50999AC52809D651C9CC5A5FDC71302C|skfA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGTAAACCTCCTCTCA, downstream forward: _UP4_TAACATTTGAGAATAGGGAG
  • BKK01910 (Δ[gene|DDB0022F50999AC52809D651C9CC5A5FDC71302C|skfA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGTAAACCTCCTCTCA, downstream forward: _UP4_TAACATTTGAGAATAGGGAG

    • GFP fusion

  • GP1645 ''[gene|154862E3FFA7CEBA5725BAD08E09D0E1C6B7F946|ymdB]''::''spc'' P''skf-gfp'' (''cat'') in the NCIB3610 background, available in [SW| Jörg Stülke]'s lab

    • References

    Reviews

  • 20955377