function
killing of sister cells
product
spore killing factor
Genomic Context
categories
[category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.17|Toxins, antitoxins and immunity against toxins][category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.4|Prophage 1][SW|Categories] containing this gene/protein
[SW|toxins, antitoxins and immunity against toxins], [SW|prophage 1]This gene is a member of the following [SW|regulons]
[SW|AbrB regulon], [SW|PhoP regulon], [SW|Spo0A regulon]Gene
Coordinates on the chromosome (coding sequence)
213,941 -> 214,108
The protein
Modification
the mature SKF is cyclic 26-amino acid peptide that is posttranslationally modified with one disulfide and one cysteine thioether bridged to the alpha-position of a methionine [Pubmed|20805502], the thioether bond is generated by [protein|search|SkfB] [Pubmed|23282011][SW|Localization]
secreted (according to Swiss-Prot)[SW|Interactions]
[protein|search|SkfA]-[protein|search|SkfB] [Pubmed|23282011]Expression and Regulation
Operon
''[protein|search|skfA]-[protein|search|skfB]-[protein|search|skfC]-[protein|search|skfE]-[protein|search|skfF]-[protein|search|skfG]-[protein|search|skfH]'' [Pubmed|16816204][SW|Sigma factor]
[protein|search|SigA] [Pubmed|16452424]Regulation
repressed by glucose (33-fold) [Pubmed|12850135]expressed under conditions that trigger sporulation ([SW|Spo0A]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647,15687200 PubMed]repressed during logrithmic growth ([protein|search|AbrB]) [http://www.ncbi.nlm.nih.gov/sites/entrez/15687200,17720793 PubMed]induced by phosphate starvation ([protein|search|PhoP]) [Pubmed|16816204]induced by rhamnogalacturonan [Pubmed|17449691]Regulatory mechanism
[SW|Spo0A]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647,15687200 PubMed][protein|search|AbrB]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/15687200,17720793 PubMed][protein|search|PhoP]: transcription activation [Pubmed|16816204]Additional information
Northern blotting during during phosphate limitation showed an intense 0.25 kb '[protein|search|skfA]'-specific transcript, and a weaker 6.5 kb '[protein|search|skfA]-[protein|search|skfB]-[protein|search|skfC]-[protein|search|skfE]-[protein|search|skfF]-[protein|search|skfG]-[protein|search|skfH]' transcript.The '[protein|search|skfA]' transcript is stabilized by a stem-loop structure in the '[protein|search|skfA]-[protein|search|skfB]' intergenic region. [http://www.ncbi.nlm.nih.gov/pubmed/16816204 PubMed]The mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]Biological materials
GFP fusion
GP1645 ''[protein|search|ymdB]''::''spc'' P''skf-gfp'' (''cat'') in the NCIB3610 background, available in [SW| Jörg Stülke]'s labReferences
