SubtiBank SubtiBank
metS [2020-04-29 16:06:27]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

metS [2020-04-29 16:06:27]

methionyl-tRNA synthetase
locus
BSU_00380
pI
4.96
mw
76.01 kDa
protein length
664 aa Sequence Blast
gene length
1995 bp Sequence Blast
function
translation
product
methionyl-tRNA synthetase
essential
yes
ec
6.1.1.10
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

    • Gene

    Coordinates
    45,633 → 47,627

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • ATP + L-methionine + tRNAMet --> AMP + diphosphate + L-methionyl-tRNAMet (according to UniProt)

    • Protein family

  • [SW|Class-I aminoacyl-tRNA synthetase family] (according to UniProt)

    • [SW|Domains]

  • [SW|tRNA-binding domain] (aa 570-662) (accordinig to UniProt)

    • Modification

  • phosphorylated on Ser-652 [Pubmed|20509597]

    • Structure

  • [PDB|1WE0] (from ''Thermus thermophilus hb8 mutant'', 40% identity, 57% similarity) [Pubmed|10673435]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|DCBDD135F295B04FE7C7EB92E94638701CB320DD|metS]-[gene|00798BB5A769C464CC5FDDF7D9E41D30B473B9A8|yabD]
    description
    [Pubmed|22383849]

    regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

    • regulation

  • repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]

    • additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of [gene|DCBDD135F295B04FE7C7EB92E94638701CB320DD|metS] [Pubmed|27120414]
    • view in new tab

    genes
    [gene|DCBDD135F295B04FE7C7EB92E94638701CB320DD|metS]

    regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

    • regulation

  • repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]

    • additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of [gene|DCBDD135F295B04FE7C7EB92E94638701CB320DD|metS] [Pubmed|27120414]
    • view in new tab

    References

  • 14651647,20509597,27120414