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murAA [Thu Jan 07 2016 19:59:27 GMT+0100 (CET)]
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murAA [Thu Jan 07 2016 19:59:27 GMT+0100 (CET)]

UDP-N-acetylglucosamine 1-carboxyvinyltransferase
locus
BSU36760
pI
5.00
mw
46.00 kDa
function
peptidoglycan precursor biosynthesis
product
UDP-N-acetylglucosamine 1-carboxyvinyltransferase
essential
yes
ec
2.5.1.7
synonyms
murA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • [SW|Categories] containing this gene/protein

  • [SW|cell wall synthesis], [SW|biosynthesis of cell wall components], [SW|essential genes]

    • Gene

    Coordinates on the chromosome (coding sequence)
    3,777,949 -> 3,779,259

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • Phosphoenolpyruvate UDP-N-acetyl-D-glucosamine = phosphate UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine (according to Swiss-Prot)

    • Protein family

  • MurA subfamily (according to Swiss-Prot)

    • Paralogous protein(s)

  • [SW|MurAB]

    • Structure

  • [PDB|3SG1] (from ''B. anthracis'', 79% identity, 94% similarity)

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Additional information

  • :
  • [SW|MurAA] is subject to [protein|search|ClpC]-[protein|search|ClpP]-dependent degradation upon glucose starvation [Pubmed|14763982,17981983]

    • Expression and Regulation

    Operon

  • ''[SW|ywzB]-[SW|spoIIT]-[SW|murAA]'' [Pubmed|22383849]
  • ''[SW|spoIIT]-[SW|murAA]'' [Pubmed|26735940]
  • ''[SW|murAA]'' [Pubmed|9353933]

    • [SW|Sigma factor]

  • ''[SW|ywzB]'': [SW|SigA] [Pubmed|22383849]
  • ''[SW|spoIIT]'': [SW|SigF] [Pubmed|26735940]

    • Regulation

  • expressed early during sporulation in the forespore ([protein|search|SigF]) [Pubmed|26735940]

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]

    • Biological materials

    Expression vector

  • pRSETA-murAA, Expression of His(6)-MurAA, avalable in [SW|Ulf Gerth]'s lab [Pubmed|14763982]
  • pGP2597: (IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab

    • Antibody

  • avalable in [SW|Ulf Gerth]'s lab [Pubmed|14763982]

    • References

    Reviews

  • 21822642

    • Original publications

  • 14763982,18063720,19899805,14763982,8994972,9485407,26735940,22383849