SubtiBank SubtiBank
adaB [2019-06-17 12:42:00]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

adaB [2019-06-17 12:42:00]

O6-methylguanine-DNA methyltransferase
locus
BSU01820
pI
6.15
mw
19.98 kDa
protein length
179 aa Sequence Blast
gene length
540 bp Sequence Blast
function
adaptive response to alkylative DNA damage
product
O6-methylguanine-DNA methyltransferase
essential
no
ec
2.1.1.63
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
  • [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.4|Prophage 1]

    • Gene

    Coordinates
    204,351 → 204,890

    The protein

    Catalyzed reaction/ biological activity

  • DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine (according to Swiss-Prot)

    • Protein family

  • MGMT family (according to Swiss-Prot)

    • Structure

  • [PDB|4BHB] (from Mycobacterium tuberculosis, 42% identity) [pubmed|23564173]

    • Expression and Regulation

    Operons

    genes
    [gene|0F8A564256A598DB3A36668FA0C984542FE1323F|adaA]-[gene|D7E4E4A95CFDB3CFCC7432B08653644AF6876CA1|adaB]
    description
    [Pubmed|2120677]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2120677], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|0F8A564256A598DB3A36668FA0C984542FE1323F|AdaA]: positive regulation, in [regulon|0F8A564256A598DB3A36668FA0C984542FE1323F|AdaA regulon]

    • regulation

  • positive control by [protein|search|AdaA] [Pubmed|2120677]
    • view in new tab

    Biological materials

    Mutant

  • BKE01820 (Δ[gene|D7E4E4A95CFDB3CFCC7432B08653644AF6876CA1|adaB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAGTAAAGACCAATAAAGGG, downstream forward: _UP4_TGAAAGTGAATCATCTATAA
  • BKK01820 (Δ[gene|D7E4E4A95CFDB3CFCC7432B08653644AF6876CA1|adaB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAGTAAAGACCAATAAAGGG, downstream forward: _UP4_TGAAAGTGAATCATCTATAA

    • References

    Reviews

  • 22933559,24810496

    • Original publications

  • 2120677,23564173