SubtiBank SubtiBank
ywjA
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ywjA

similar to [SW|ABC transporter] (ATP-binding protein)
locus
BSU_37230
pI
6.14
mw
64.39 kDa
protein length
575 aa Sequence Blast
gene length
1728 bp Sequence Blast
function
unknown
product
[SW|ABC transporter] (ATP-binding protein)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.2|Exporters] → [category|SW 1.2.1.2.6|Exporters of unknown function]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,819,754 → 3,821,481

    The protein

    Protein family

  • [SW|ABC transporter superfamily] (according to UniProt)

    • Paralogous protein(s)

  • [protein|E85814EDF232D21A42380D559EAD2CDFB41F19DB|YfiC], [protein|ED1F82463BAA28B67184BFAB5CB23CF26A62999D|BmrA], [protein|70D7098BEBD5E53B4B9B8C3394FAAF972C65EE22|YknU], [protein|C6B0ACB9D9703DD8141FD7D9D62DB22FB48F458B|YknV], [protein|0EB4D3E1B9AF39ECCBD79EB559FAE5BB0EE4C156|YgaD], [protein|67C0C9EA17EF953554D3D57CD0D285246F50C1AD|BmrC], [protein|288CA73D93B1E5106E1C6CE5E7E2E8BE2A6BD3F3|YfiB]

    • [SW|Domains]

  • has both a membrane-spanning and an ATP-binding domain [Pubmed|10092453]
  • [SW|ABC transmembrane type-1 domain] (aa 16-299) (according to UniProt)
  • [SW|ABC transporter domain] (aa 333-567) (according to UniProt)

    • Structure

  • [PDB|2HYD] (from Staphylococcus aureus, 38% identity) [pubmed|16943773]

    • [SW|Localization]

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|07752EC6940F43DEBD1E9313A2A195E35EDB9F69|ywiE]-[gene|D5A3C316BD4567A0155F70330E32ACDE18F063FE|ywjA]-[gene|685B58AD2A02A0B48B7D4AAAE046DE2CF8F77C58|ywjB]
    description
    [pubmed|22383849]

    sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|26577401], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

    • regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]
    • view in new tab

    genes
    [gene|D5A3C316BD4567A0155F70330E32ACDE18F063FE|ywjA]-[gene|685B58AD2A02A0B48B7D4AAAE046DE2CF8F77C58|ywjB]
    description
    [pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • MGNA-B662 (ywjA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1661 NBRP B. subtilis, Japan]
  • BKE37230 (Δ[gene|D5A3C316BD4567A0155F70330E32ACDE18F063FE|ywjA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCCTATCTTCCCCCAATG, downstream forward: _UP4_ATGGTGCATCGATAGGAGGA
  • BKK37230 (Δ[gene|D5A3C316BD4567A0155F70330E32ACDE18F063FE|ywjA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCCTATCTTCCCCCAATG, downstream forward: _UP4_ATGGTGCATCGATAGGAGGA

    • References

  • 10092453,12354229,26577401,16943773