SubtiBank SubtiBank
dnaJ
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dnaJ

heat-shock protein (activation of [protein|30E0AAABB803E577D0D9FBAEF9031319CABD3D89|DnaK])
locus
BSU_25460
pI
7.92
mw
40.69 kDa
protein length
375 aa Sequence Blast
gene length
1128 bp Sequence Blast
function
protein quality control
product
heat-shock protein (activation of [protein|30E0AAABB803E577D0D9FBAEF9031319CABD3D89|DnaK])
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.2|Chaperones/ protein folding]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]

    • Gene

    Coordinates
    2,624,785 → 2,625,912

    The protein

    Protein family

  • dnaJ family (single member, according to UniProt)

    • [SW|Domains]

  • J domain (aa 5-69) (according to UniProt)

    • Structure

  • [PDB|3LZ8] (from ''Klebsiella pneumoniae'', 34% identity)

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|hrcA]-[gene|ECAB685E9038DC03FA2CC659112BB07D30DE5C8C|grpE]-[gene|30E0AAABB803E577D0D9FBAEF9031319CABD3D89|dnaK]-[gene|D4C75B39B2DAECB9D85DAD66AAD4F923C9AEBEA7|dnaJ]-[gene|70D717806F5174447E01FF33DC8299CB025888CD|yqeT]-[gene|F8DC71B29D9C9BF4CF5124D0F01A71548306B9F9|yqeU]-[gene|6ADA5AF308D96218414EA92DFF4A57BAEAD0554B|yqeV]
    description
    [Pubmed|9023197]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1339421], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|HrcA]: repression, [Pubmed|1339421], in [regulon|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|HrcA regulon]

    • regulation

  • induced by heat shock ([protein|search|HrcA]) [Pubmed|1339421]
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    Biological materials

    Mutant

  • BKE25460 (Δ[gene|D4C75B39B2DAECB9D85DAD66AAD4F923C9AEBEA7|dnaJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCGCTTCACTCTCCCG, downstream forward: _UP4_TAATTATTGGATGAGGAGTT
  • BKK25460 (Δ[gene|D4C75B39B2DAECB9D85DAD66AAD4F923C9AEBEA7|dnaJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCGCTTCACTCTCCCG, downstream forward: _UP4_TAATTATTGGATGAGGAGTT

    • labs

  • [SW|Wolfgang Schumann], Bayreuth University, Germany [http://www.genetik.uni-bayreuth.de/LSGenetik1/schumann_research.htm Homepage]

    • References

  • 10383760,9023197,1339421,7592421