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yfjQ [2017-10-25 13:09:03]
minor magnesium transporter
function
magnesium uptake
product
minor magnesium transporter
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.1|Magnesium uptake/ efflux][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]Gene
Coordinates
871,347 → 872,306
Phenotypes of a mutant
a ''[gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE] [gene|D281B88EC444F8175AB50FB2CD24B51F4E2C96F8|yfjQ]'' mutant does not grow on rich media, this can be suppressed by the addition of 25 mM Mg2+, moreover the mutant grows well on minimal medium in the presence of citrate (due to the activity of [protein|C0DED68802EF78D2BC16507234AAC3B60D236E68|CitM]) [Pubmed|24415722]The protein
Structure
[PDB|4I0U] (from Thermotoga maritima, 37% identity) [pubmed|23425532]Expression and Regulation
Operons
genes
[gene|D281B88EC444F8175AB50FB2CD24B51F4E2C96F8|yfjQ]
description
[pubmed|22383849]
Biological materials
Mutant
MGNA-C274 (yfjQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2272 NBRP B. subtilis, Japan]BKE08000 (Δ[gene|D281B88EC444F8175AB50FB2CD24B51F4E2C96F8|yfjQ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAACAACCCTCCACCTG, downstream forward: _UP4_TGAGGACAGCGAGCAGCTGTBKK08000 (Δ[gene|D281B88EC444F8175AB50FB2CD24B51F4E2C96F8|yfjQ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAACAACCCTCCACCTG, downstream forward: _UP4_TGAGGACAGCGAGCAGCTGTReferences
