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conE
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conE

ATPase, required for conjugation of ICEBs1, part of the type IV secretion system for DNA transfer
locus
BSU_04940
pI
5.51
mw
96.06 kDa
protein length
797 aa Sequence Blast
gene length
2394 bp Sequence Blast
function
conjugative transfer of ICEBs1
product
ATPase, part of the type IV secretion system for DNA transfer
essential
no
synonyms
yddE
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.2|Mobile genetic elements] → [category|SW 5.2.1|ICEBs1]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    538,836 → 541,229

    Phenotypes of a mutant

  • Defective for conjugal transfer of ICE''Bs1

    • The protein

    Catalyzed reaction/ biological activity

  • required for conjugative transfer of ICEBs1, putative ATPase [Pubmed|19734305]

    • Protein family

  • HerA/FtsK Superfamily of ATPases including the well-studied proteins FtsK, SpoIIIE, VirB4, and VirD4

    • [SW|Localization]

  • peripheral membrane protein (via [protein|A0AFD543582D2FE8B1139CF69B7B1F7FF0F80265|ConB]), located at the cell poles [Pubmed|26013486,19734305]

    • Expression and Regulation

    Operons

    genes
    [gene|EE2A4068CBD7A2F852E582E2EDA4148381F1CD61|xis]-[gene|2227923C603B290AB276238F789D71584FB07DF3|ydzL]-[gene|34451538F10B4ABCFC8A236C8C905A20D989EBCF|ydcO]-[gene|C042792D9A643DCFF4A851EFECA3CF2C31E1EDAF|ydcP]-[gene|4BCAA5939CB7F0DF23495BC92C9EF0A0149296A0|ydcQ]-[gene|8E179976C35FE09F92A191D00E497ED4BF28F571|nicK]-[gene|886B2132717BA4C72433ABE351C5791C9DE1432A|S172]-[gene|1699CFBF5400CAF43C6DAE8ADCCE5A360B31F2E9|ydcS]-[gene|9D4918852A1856B1CDD72D49AEA75E51A8602BB6|ydcT]-[gene|62CEABA069CA2970F22B7E0C369D540A2A027A8D|yddA]-[gene|A0AFD543582D2FE8B1139CF69B7B1F7FF0F80265|conB]-[gene|F273A8817E66B44D58FC2BCB6576A30D8616B750|conC]-[gene|F1F898D7A9FDF78D2E5E8F3BF605E0F735D0542A|conD]-[gene|D20F724D8C3E8C758A9FADF133CD43F066BD8D48|conE]-[gene|B3A07E2A61151C33B7C65D1BC58B97BFDB76852D|yddF]-[gene|3356A43B83E2D4D66098F17A751F0912C1553398|conG]-[gene|8D9D81E05BAA8697F002BB1B8DBC5ABBA557353F|cwlT]-[gene|2E4FF80C857B3F40BA835814EC40D6562721AEFE|yddI]-[gene|2FB1C9435AE924F8E450AFA81F800196A871F2D0|yddJ]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [protein|DD1C8F3A4809785BD6A6047D39B42AB2C605E161|ImmR]: repression, [Pubmed|17511812], in [regulon|DD1C8F3A4809785BD6A6047D39B42AB2C605E161|ImmR regulon]

    • regulation

  • strongly induced in the presence of salt (1.2 M NaCl) [pubmed|32419322]
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    Biological materials

    Mutant

  • BKE04940 (Δ[gene|D20F724D8C3E8C758A9FADF133CD43F066BD8D48|conE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04940 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTTTTTATACGATTCAACTT, downstream forward: _UP4_TTGTAAAAAGGGGTTGAATC
  • BKK04940 (Δ[gene|D20F724D8C3E8C758A9FADF133CD43F066BD8D48|conE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04940 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTTTTTATACGATTCAACTT, downstream forward: _UP4_TTGTAAAAAGGGGTTGAATC

    • labs

  • [SW|Melanie Barker Berkmen], Suffolk University, Boston, MA [http://openwetware.org/wiki/Berkmen Homepage]

    • References

  • 19734305,26013486