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speD [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]
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speD [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]

S-adenosylmethionine decarboxylase, required for biofilm formation
locus
BSU29010
pI
4.00
mw
13.00 kDa
function
spermidine, polyamine biosynthesis
product
S-adenosylmethionine decarboxylase
essential
no
ec
4.1.1.50
synonyms
ytcF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.6|Metabolism of polyamines]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.5|Other proteins required for biofilm formation]

    • [SW|Categories] containing this gene/protein

  • [SW|miscellaneous metabolic pathways], [SW|biofilm formation]

    • This gene is a member of the following [SW|regulons]

  • [SW|CcpN regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,966,413 -> 2,966,793

    Phenotypes of a mutant

  • no [SW|biofilm formation] [Pubmed|24529384]

    • The protein

    Catalyzed reaction/ biological activity

  • S-adenosyl-L-methionine = (5-deoxy-5-adenosyl)(3-aminopropyl)-methylsulfonium salt CO2 (according to Swiss-Prot)

    • Protein family

  • Type 1 subfamily (according to Swiss-Prot)

    • Structure

  • [PDB|1VR7] (from ''Thermotoga maritima'', 47% identity, 72% similarity)

    • Expression and Regulation

    Operon

  • ''[protein|search|gapB]-[protein|search|speD]'' [Pubmed|15720552]
  • ''[protein|search|speD]'' [Pubmed|10844697]

    • [SW|Sigma factor]

  • ''[protein|search|gapB]'': [protein|search|SigA] [Pubmed|15720552]
  • ''[protein|search|speD]'': [protein|search|SigA] [Pubmed|10844697]

    • Regulation

  • ''[protein|search|gapB]'': repressed in the presence of glucose ([protein|search|CcpN]) [Pubmed|15720552]

    • Regulatory mechanism

  • [protein|search|CcpN]: transcription repression [Pubmed|15720552]

    • Additional information

  • '[protein|search|speD]': the mRNA is substantially stabilized upon depletion of [Rny|search|RNase Y] (the half-life of the monocistronic 'speD' mRNA increases from 1.4 to 36 min) [PubMed|21815947]

    • Biological materials

    Mutant

  • MGNA-A122 (ytcF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/122 NBRP B. subtilis, Japan]

    • References

  • 10844697,15720552,9723923,21815947