SubtiBank SubtiBank
thiF [2017-8-11 16:0:3]
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thiF [2017-8-11 16:0:3]

hydroxyethylthiazole phosphate biosynthesis
locus
BSU11700
pI
5.62
mw
36.24 kDa
protein length
336 aa Sequence Blast
gene length
1008 bp Sequence Blast
function
biosynthesis of thiamine
product
unknown
essential
no
synonyms
yjbU
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.3|Biosynthesis/ acquisition of thiamine]

    • Gene

    Coordinates
    1,245,808 → 1,246,818

    The protein

    Paralogous protein(s)

  • [protein|C163CF34BAA3A37DCC67851DEE5889BB7AD208AB|MoeB]

    • Structure

  • [PDB|1ZFN] (from ''E. coli'', 35% identity, 51% similarity) [Pubmed|15896804]

    • Expression and Regulation

    Operons

    genes
    [gene|47E47FB7D9C079DF603906694C4649A049814346|tenA]-[gene|CA6AAE8AC5B5EF01A911B035F8782579CD876C08|tenI]-[gene|87B5C84B7EE10F60B545CCFEF6A527A546B80432|thiO]-[gene|95DA3F899037140F48B99E065C768356CE08FA94|thiS]-[gene|B58B8F260E446E28BC2E3A9AA84EA771EC0A8634|thiG]-[gene|CCD3DDE9E52FCD4906735059CF30593BEB56FD1B|thiF]-[gene|97EDB4ACD64C5FA2E6014ED133DF18F602FF58BB|thiD]
    description
    [Pubmed|1898926]

    regulatory mechanism

  • [protein|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box]: [SW|RNA switch], via [SW|RNA switch], in [regulon|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box regulon]

    • regulation

  • repressed by thiamine ([SW|Thi-box]) [Pubmed|16356850]
  • the [SW|Thi-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
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    Biological materials

    Mutant

  • MGNA-B166 (yjbU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1165 NBRP B. subtilis, Japan]

    • References

    Reviews

  • 19348578,10382260

    • Original publications

  • 14567704,15896804,1898926,16356850